Development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration: A comparative study
In severe peripheral nerve injuries, nerve conduits (NCs) are good alternatives to autografts/allografts; however, the results the available devices guarantee for are still not fully satisfactory. Herein, differently bioactivated NCs based on the new polymer oxidized polyvinyl alcohol (OxPVA) are co...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006423002211 |
| _version_ | 1827815417504071680 |
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| author | Elena Stocco Silvia Barbon Diego Faccio Lucia Petrelli Damiana Incendi Annj Zamuner Enrico De Rose Marta Confalonieri Francesco Tolomei Silvia Todros Cesare Tiengo Veronica Macchi Monica Dettin Raffaele De Caro Andrea Porzionato |
| author_facet | Elena Stocco Silvia Barbon Diego Faccio Lucia Petrelli Damiana Incendi Annj Zamuner Enrico De Rose Marta Confalonieri Francesco Tolomei Silvia Todros Cesare Tiengo Veronica Macchi Monica Dettin Raffaele De Caro Andrea Porzionato |
| author_sort | Elena Stocco |
| collection | DOAJ |
| description | In severe peripheral nerve injuries, nerve conduits (NCs) are good alternatives to autografts/allografts; however, the results the available devices guarantee for are still not fully satisfactory. Herein, differently bioactivated NCs based on the new polymer oxidized polyvinyl alcohol (OxPVA) are compared in a rat model of sciatic nerve neurotmesis (gap: 5 mm; end point: 6 weeks). Thirty Sprague Dawley rats are randomized to 6 groups: Reverse Autograft (RA); Reaxon®; OxPVA; OxPVA + EAK (self-assembling peptide, mechanical incorporation); OxPVA + EAK-YIGSR (mechanical incorporation); OxPVA + Nerve Growth Factor (NGF) (adsorption). Preliminarily, all OxPVA-based devices are comparable with Reaxon® in Sciatic Functional Index score and gait analysis; moreover, all conduits sustain nerve regeneration (S100, β-tubulin) without showing substantial inflammation (CD3, F4/80) evidences. Following morphometric analyses, OxPVA confirms its potential in PNI repair (comparable with Reaxon®) whereas OxPVA + EAK-YIGSR stands out for its myelinated axons total number and density, revealing promising in injury recovery and for future application in clinical practice. |
| first_indexed | 2024-03-11T23:59:51Z |
| format | Article |
| id | doaj.art-28c3974ec6f64be19480a6e60c5c60e9 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-03-11T23:59:51Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-28c3974ec6f64be19480a6e60c5c60e92023-09-18T04:30:19ZengElsevierMaterials Today Bio2590-00642023-10-0122100761Development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration: A comparative studyElena Stocco0Silvia Barbon1Diego Faccio2Lucia Petrelli3Damiana Incendi4Annj Zamuner5Enrico De Rose6Marta Confalonieri7Francesco Tolomei8Silvia Todros9Cesare Tiengo10Veronica Macchi11Monica Dettin12Raffaele De Caro13Andrea Porzionato14Department of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy; Department of Cardiac, Thoracic and Vascular Science and Public Health, University of Padova, Via Nicolò Giustiniani 2, 35128, Padova, Italy; L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, ItalyDepartment of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy; L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, ItalyPlastic and Reconstructive Surgery Unit, University of Padova, Via Nicolò Giustiniani 2, 35128, Padova, ItalyDepartment of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, ItalyDepartment of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, ItalyL.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, Italy; Department of Civil, Environmental and Architectural Engineering University of Padova, Via Francesco Marzolo 9, 35131, Padova, Italy; Department of Industrial Engineering University of Padova, Via Gradenigo 6/a, 35131, Padova, ItalyDepartment of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, ItalyDepartment of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy; Department of Industrial Engineering University of Padova, Via Gradenigo 6/a, 35131, Padova, ItalyDepartment of Industrial Engineering University of Padova, Via Gradenigo 6/a, 35131, Padova, ItalyDepartment of Industrial Engineering University of Padova, Via Gradenigo 6/a, 35131, Padova, ItalyPlastic and Reconstructive Surgery Unit, University of Padova, Via Nicolò Giustiniani 2, 35128, Padova, ItalyDepartment of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy; L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, ItalyL.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, Italy; Department of Industrial Engineering University of Padova, Via Gradenigo 6/a, 35131, Padova, ItalyDepartment of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy; L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, Italy; Corresponding author. Department of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy.Department of Neurosciences, Section of Human Anatomy, University of Padova, Via Aristide Gabelli 65, 35127, Padova, Italy; L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Nicolò Giustiniani 2, 35128, Padova, ItalyIn severe peripheral nerve injuries, nerve conduits (NCs) are good alternatives to autografts/allografts; however, the results the available devices guarantee for are still not fully satisfactory. Herein, differently bioactivated NCs based on the new polymer oxidized polyvinyl alcohol (OxPVA) are compared in a rat model of sciatic nerve neurotmesis (gap: 5 mm; end point: 6 weeks). Thirty Sprague Dawley rats are randomized to 6 groups: Reverse Autograft (RA); Reaxon®; OxPVA; OxPVA + EAK (self-assembling peptide, mechanical incorporation); OxPVA + EAK-YIGSR (mechanical incorporation); OxPVA + Nerve Growth Factor (NGF) (adsorption). Preliminarily, all OxPVA-based devices are comparable with Reaxon® in Sciatic Functional Index score and gait analysis; moreover, all conduits sustain nerve regeneration (S100, β-tubulin) without showing substantial inflammation (CD3, F4/80) evidences. Following morphometric analyses, OxPVA confirms its potential in PNI repair (comparable with Reaxon®) whereas OxPVA + EAK-YIGSR stands out for its myelinated axons total number and density, revealing promising in injury recovery and for future application in clinical practice.http://www.sciencedirect.com/science/article/pii/S2590006423002211Peripheral nerve injuriesNerve conduitsBioactivationNerve regenerationSelf-assembling peptides |
| spellingShingle | Elena Stocco Silvia Barbon Diego Faccio Lucia Petrelli Damiana Incendi Annj Zamuner Enrico De Rose Marta Confalonieri Francesco Tolomei Silvia Todros Cesare Tiengo Veronica Macchi Monica Dettin Raffaele De Caro Andrea Porzionato Development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration: A comparative study Materials Today Bio Peripheral nerve injuries Nerve conduits Bioactivation Nerve regeneration Self-assembling peptides |
| title | Development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration: A comparative study |
| title_full | Development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration: A comparative study |
| title_fullStr | Development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration: A comparative study |
| title_full_unstemmed | Development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration: A comparative study |
| title_short | Development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration: A comparative study |
| title_sort | development and preclinical evaluation of bioactive nerve conduits for peripheral nerve regeneration a comparative study |
| topic | Peripheral nerve injuries Nerve conduits Bioactivation Nerve regeneration Self-assembling peptides |
| url | http://www.sciencedirect.com/science/article/pii/S2590006423002211 |
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