Complementary transcriptome and proteome profiling in the mature seeds of Camellia oleifera from Hainan Island.
Camellia oleifera Abel. (C. oleifera), as an important woody tree species producing edible oils in China, has attracted enormous attention due to its abundant unsaturated fatty acids and their associated benefits to human health. To reveal novel insights into the characters during the maturation per...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0226888 |
| _version_ | 1818833782995681280 |
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| author | Zhouchen Ye Yougen Wu Zeeshan Ul Haq Muhammad Wuping Yan Jing Yu Junfeng Zhang Guanglong Yao Xinwen Hu |
| author_facet | Zhouchen Ye Yougen Wu Zeeshan Ul Haq Muhammad Wuping Yan Jing Yu Junfeng Zhang Guanglong Yao Xinwen Hu |
| author_sort | Zhouchen Ye |
| collection | DOAJ |
| description | Camellia oleifera Abel. (C. oleifera), as an important woody tree species producing edible oils in China, has attracted enormous attention due to its abundant unsaturated fatty acids and their associated benefits to human health. To reveal novel insights into the characters during the maturation period of this plant as well as the molecular basis of fatty acid biosynthesis and degradation, we conducted a conjoint analysis of the transcriptome and proteome of C. oleifera seeds from Hainan Island. Using RNA sequencing (RNA-seq) technology and shotgun proteomic method, 59,391 transcripts and 40,500 unigenes were obtained by TIGR Gene Indices Clustering Tools (TGICL), while 1691 protein species were identified from Mass Spectrometry (MS). Subsequently, all genes and proteins were employed in euKaryotic Orthologous Groups (KOG) classification, Gene Ontology (GO) annotation, and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis to investigate their essential functions. The results indicated that the most abundant pathways were biological metabolic processes. There were 946 unigenes associated with lipid metabolism at the transcriptome level, with 116 proteins at the proteome level; among these, 38 specific proteins were involved in protein-protein interactions, with the majority being related to fatty acid catabolic process. The expression levels of 21 candidate unigenes encoding target proteins were further detected by quantitative real-time polymerase chain reaction (qRT-PCR). Finally, Gas Chromatography Mass Spectrometry (GC-MS) was carried out to determine the fatty acid composition of C. oleifera oil. These findings not only deepened our understanding about the molecular mechanisms of fatty acid metabolism but also offered new evidence concerning the roles of relevant proteins in oil-bearing crops. Furthermore, the lipid-associated proteins recognized in this research might be helpful in providing a reference for the synthetic regulation of C. oleifera oil quality by genetic engineering techniques, thus resulting in potential application in agriculture. |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T02:24:24Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-28c6a6a12a4d43b1b2df83f30d8ec4622022-12-21T20:39:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01152e022688810.1371/journal.pone.0226888Complementary transcriptome and proteome profiling in the mature seeds of Camellia oleifera from Hainan Island.Zhouchen YeYougen WuZeeshan Ul Haq MuhammadWuping YanJing YuJunfeng ZhangGuanglong YaoXinwen HuCamellia oleifera Abel. (C. oleifera), as an important woody tree species producing edible oils in China, has attracted enormous attention due to its abundant unsaturated fatty acids and their associated benefits to human health. To reveal novel insights into the characters during the maturation period of this plant as well as the molecular basis of fatty acid biosynthesis and degradation, we conducted a conjoint analysis of the transcriptome and proteome of C. oleifera seeds from Hainan Island. Using RNA sequencing (RNA-seq) technology and shotgun proteomic method, 59,391 transcripts and 40,500 unigenes were obtained by TIGR Gene Indices Clustering Tools (TGICL), while 1691 protein species were identified from Mass Spectrometry (MS). Subsequently, all genes and proteins were employed in euKaryotic Orthologous Groups (KOG) classification, Gene Ontology (GO) annotation, and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis to investigate their essential functions. The results indicated that the most abundant pathways were biological metabolic processes. There were 946 unigenes associated with lipid metabolism at the transcriptome level, with 116 proteins at the proteome level; among these, 38 specific proteins were involved in protein-protein interactions, with the majority being related to fatty acid catabolic process. The expression levels of 21 candidate unigenes encoding target proteins were further detected by quantitative real-time polymerase chain reaction (qRT-PCR). Finally, Gas Chromatography Mass Spectrometry (GC-MS) was carried out to determine the fatty acid composition of C. oleifera oil. These findings not only deepened our understanding about the molecular mechanisms of fatty acid metabolism but also offered new evidence concerning the roles of relevant proteins in oil-bearing crops. Furthermore, the lipid-associated proteins recognized in this research might be helpful in providing a reference for the synthetic regulation of C. oleifera oil quality by genetic engineering techniques, thus resulting in potential application in agriculture.https://doi.org/10.1371/journal.pone.0226888 |
| spellingShingle | Zhouchen Ye Yougen Wu Zeeshan Ul Haq Muhammad Wuping Yan Jing Yu Junfeng Zhang Guanglong Yao Xinwen Hu Complementary transcriptome and proteome profiling in the mature seeds of Camellia oleifera from Hainan Island. PLoS ONE |
| title | Complementary transcriptome and proteome profiling in the mature seeds of Camellia oleifera from Hainan Island. |
| title_full | Complementary transcriptome and proteome profiling in the mature seeds of Camellia oleifera from Hainan Island. |
| title_fullStr | Complementary transcriptome and proteome profiling in the mature seeds of Camellia oleifera from Hainan Island. |
| title_full_unstemmed | Complementary transcriptome and proteome profiling in the mature seeds of Camellia oleifera from Hainan Island. |
| title_short | Complementary transcriptome and proteome profiling in the mature seeds of Camellia oleifera from Hainan Island. |
| title_sort | complementary transcriptome and proteome profiling in the mature seeds of camellia oleifera from hainan island |
| url | https://doi.org/10.1371/journal.pone.0226888 |
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