Pathogenic Th2 Cytokine Profile Skewing by IFN-γ-Responding Vitiligo Fibroblasts via CCL2/CCL8
Purpose: Vitiligo is a T cell-mediated skin depigmentation disease. Though treatments arresting disease progression and inducing repigmentation are available, the efficacy of these options is often limited and poorly sustained. How stromal signals contribute to the interferon-γ-dominant skin niches...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/2/217 |
| _version_ | 1797444490947985408 |
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| author | Rong Jin Miaoni Zhou Fuquan Lin Wen Xu Aie Xu |
| author_facet | Rong Jin Miaoni Zhou Fuquan Lin Wen Xu Aie Xu |
| author_sort | Rong Jin |
| collection | DOAJ |
| description | Purpose: Vitiligo is a T cell-mediated skin depigmentation disease. Though treatments arresting disease progression and inducing repigmentation are available, the efficacy of these options is often limited and poorly sustained. How stromal signals contribute to the interferon-γ-dominant skin niches is unclear. This study aims to determine how fibroblasts participate in the IFN-γ-dominant vitiligo niche. Patients and methods: Mouse vitiligo models were established. Fibroblasts from control and vitiligo mice were extracted for RNA sequencing. In vitro IFN-γ stimulation was performed to verify the JAK-STAT pathway by qPCR and Western blot. T cell polarization with chemokines was measured by flow cytometry. Protein levels in tissues were also examined by IHC. Results: The vitiligo mouse model recapitulates the human CD8-IFN-γ pathway. RNA sequencing revealed elevated chemokine CCL2 and CCL8 in vitiligo fibroblast, which may be regulated by the JAK-STAT signaling. Such phenomenon is verified by JAK inhibitor peficitinib in vitro. Moreover, CCL2 addition into the naïve T polarization system promoted type 2 cytokines secretion, which represents a hallmark of vitiligo lesions. Conclusion: Dermal fibroblasts, a principal constituent of skin structure, respond to IFN-γ by skewing T cells towards a type 2 cytokine profile via CCL2 and CCL8, which can be abrogated by JAK inhibitor peficitinib. |
| first_indexed | 2024-03-09T13:13:13Z |
| format | Article |
| id | doaj.art-28c7d2dc77924811b2d6f8fb7db00da1 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T13:13:13Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-28c7d2dc77924811b2d6f8fb7db00da12023-11-30T21:39:10ZengMDPI AGCells2073-44092023-01-0112221710.3390/cells12020217Pathogenic Th2 Cytokine Profile Skewing by IFN-γ-Responding Vitiligo Fibroblasts via CCL2/CCL8Rong Jin0Miaoni Zhou1Fuquan Lin2Wen Xu3Aie Xu4Department of Dermatology, Hangzhou Third People’s Hospital, Hangzhou 310009, ChinaDepartment of Dermatology, Hangzhou Third People’s Hospital, Hangzhou 310009, ChinaDepartment of Dermatology, Hangzhou Third People’s Hospital, Hangzhou 310009, ChinaDepartment of Dermatology, Hangzhou Third People’s Hospital, Hangzhou 310009, ChinaDepartment of Dermatology, Hangzhou Third People’s Hospital, Hangzhou 310009, ChinaPurpose: Vitiligo is a T cell-mediated skin depigmentation disease. Though treatments arresting disease progression and inducing repigmentation are available, the efficacy of these options is often limited and poorly sustained. How stromal signals contribute to the interferon-γ-dominant skin niches is unclear. This study aims to determine how fibroblasts participate in the IFN-γ-dominant vitiligo niche. Patients and methods: Mouse vitiligo models were established. Fibroblasts from control and vitiligo mice were extracted for RNA sequencing. In vitro IFN-γ stimulation was performed to verify the JAK-STAT pathway by qPCR and Western blot. T cell polarization with chemokines was measured by flow cytometry. Protein levels in tissues were also examined by IHC. Results: The vitiligo mouse model recapitulates the human CD8-IFN-γ pathway. RNA sequencing revealed elevated chemokine CCL2 and CCL8 in vitiligo fibroblast, which may be regulated by the JAK-STAT signaling. Such phenomenon is verified by JAK inhibitor peficitinib in vitro. Moreover, CCL2 addition into the naïve T polarization system promoted type 2 cytokines secretion, which represents a hallmark of vitiligo lesions. Conclusion: Dermal fibroblasts, a principal constituent of skin structure, respond to IFN-γ by skewing T cells towards a type 2 cytokine profile via CCL2 and CCL8, which can be abrogated by JAK inhibitor peficitinib.https://www.mdpi.com/2073-4409/12/2/217vitiligofibroblastT cellsCCL2JAK |
| spellingShingle | Rong Jin Miaoni Zhou Fuquan Lin Wen Xu Aie Xu Pathogenic Th2 Cytokine Profile Skewing by IFN-γ-Responding Vitiligo Fibroblasts via CCL2/CCL8 Cells vitiligo fibroblast T cells CCL2 JAK |
| title | Pathogenic Th2 Cytokine Profile Skewing by IFN-γ-Responding Vitiligo Fibroblasts via CCL2/CCL8 |
| title_full | Pathogenic Th2 Cytokine Profile Skewing by IFN-γ-Responding Vitiligo Fibroblasts via CCL2/CCL8 |
| title_fullStr | Pathogenic Th2 Cytokine Profile Skewing by IFN-γ-Responding Vitiligo Fibroblasts via CCL2/CCL8 |
| title_full_unstemmed | Pathogenic Th2 Cytokine Profile Skewing by IFN-γ-Responding Vitiligo Fibroblasts via CCL2/CCL8 |
| title_short | Pathogenic Th2 Cytokine Profile Skewing by IFN-γ-Responding Vitiligo Fibroblasts via CCL2/CCL8 |
| title_sort | pathogenic th2 cytokine profile skewing by ifn γ responding vitiligo fibroblasts via ccl2 ccl8 |
| topic | vitiligo fibroblast T cells CCL2 JAK |
| url | https://www.mdpi.com/2073-4409/12/2/217 |
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