Lactoferrin mitigates ethanol-induced gastric ulcer via modulation of ROS/ICAM-1/Nrf2 signaling pathway in Wistar rats
Objective(s): We aimed to investigate the gastroprotective effect of lactoferrin (LF; 100 & 300 mg/kg) in male Wistar rats versus gastric ulcers induced by 96% ethanol.Materials and Methods: Rats were randomly allocated into 4 groups: control, ethanol, ethanol+LF100, and ethanol+LF300. LF100 &am...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2022-12-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_21185_bf7b026b4a0f5f9092c8e074bcb0a07c.pdf |
| _version_ | 1828087314837929984 |
|---|---|
| author | Gihan Asaad Rasha Mostafa |
| author_facet | Gihan Asaad Rasha Mostafa |
| author_sort | Gihan Asaad |
| collection | DOAJ |
| description | Objective(s): We aimed to investigate the gastroprotective effect of lactoferrin (LF; 100 & 300 mg/kg) in male Wistar rats versus gastric ulcers induced by 96% ethanol.Materials and Methods: Rats were randomly allocated into 4 groups: control, ethanol, ethanol+LF100, and ethanol+LF300. LF100 & 300 were given 15 days before ulcer induction. At the end of the experiment, the gastric mucosa was examined macroscopically and microscopically.Results: The ethanol group showed damage and degeneration of the stomach mucosa in addition to elevation of oxidative and inflammatory biomarkers. LF showed explicit healing of the gastric mucosal damage. LF reduced gastric malondialdehyde (MDA), tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), myeloperoxidase (MPO), and intracellular adhesion molecule-1 (ICAM-1). On the other hand, LF elevated the depleted reduced glutathione (GSH) and Nuclear factor-erythroid factor 2 (Nrf2).Conclusion: Our current study is the first to study the antiulcer effect of LF via its potential modulatory effects on the ROS/ICAM-1/Nrf2 signaling pathway. Moreover, we concluded that pretreatment with LF100 & 300 mitigated the ethanol-induced gastric ulcer via modulation of both oxidative stress and inflammatory responses. |
| first_indexed | 2024-04-11T05:10:27Z |
| format | Article |
| id | doaj.art-28c8728b0e28489b89393d319b3323b0 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-11T05:10:27Z |
| publishDate | 2022-12-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-28c8728b0e28489b89393d319b3323b02022-12-25T11:14:15ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742022-12-0125121522152710.22038/ijbms.2022.66823.1465621185Lactoferrin mitigates ethanol-induced gastric ulcer via modulation of ROS/ICAM-1/Nrf2 signaling pathway in Wistar ratsGihan Asaad0Rasha Mostafa1Department of Pharmacology, Medical Research and Clinical Studies Institute, National Research Centre, Dokki, Cairo 12622, EgyptDepartment of Pharmacology, Medical Research and Clinical Studies Institute, National Research Centre, Dokki, Cairo 12622, EgyptObjective(s): We aimed to investigate the gastroprotective effect of lactoferrin (LF; 100 & 300 mg/kg) in male Wistar rats versus gastric ulcers induced by 96% ethanol.Materials and Methods: Rats were randomly allocated into 4 groups: control, ethanol, ethanol+LF100, and ethanol+LF300. LF100 & 300 were given 15 days before ulcer induction. At the end of the experiment, the gastric mucosa was examined macroscopically and microscopically.Results: The ethanol group showed damage and degeneration of the stomach mucosa in addition to elevation of oxidative and inflammatory biomarkers. LF showed explicit healing of the gastric mucosal damage. LF reduced gastric malondialdehyde (MDA), tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), myeloperoxidase (MPO), and intracellular adhesion molecule-1 (ICAM-1). On the other hand, LF elevated the depleted reduced glutathione (GSH) and Nuclear factor-erythroid factor 2 (Nrf2).Conclusion: Our current study is the first to study the antiulcer effect of LF via its potential modulatory effects on the ROS/ICAM-1/Nrf2 signaling pathway. Moreover, we concluded that pretreatment with LF100 & 300 mitigated the ethanol-induced gastric ulcer via modulation of both oxidative stress and inflammatory responses.https://ijbms.mums.ac.ir/article_21185_bf7b026b4a0f5f9092c8e074bcb0a07c.pdfanti-oxidantanti-inflammatoryethanolgastric ulcerlactoferrin |
| spellingShingle | Gihan Asaad Rasha Mostafa Lactoferrin mitigates ethanol-induced gastric ulcer via modulation of ROS/ICAM-1/Nrf2 signaling pathway in Wistar rats Iranian Journal of Basic Medical Sciences anti-oxidant anti-inflammatory ethanol gastric ulcer lactoferrin |
| title | Lactoferrin mitigates ethanol-induced gastric ulcer via modulation of ROS/ICAM-1/Nrf2 signaling pathway in Wistar rats |
| title_full | Lactoferrin mitigates ethanol-induced gastric ulcer via modulation of ROS/ICAM-1/Nrf2 signaling pathway in Wistar rats |
| title_fullStr | Lactoferrin mitigates ethanol-induced gastric ulcer via modulation of ROS/ICAM-1/Nrf2 signaling pathway in Wistar rats |
| title_full_unstemmed | Lactoferrin mitigates ethanol-induced gastric ulcer via modulation of ROS/ICAM-1/Nrf2 signaling pathway in Wistar rats |
| title_short | Lactoferrin mitigates ethanol-induced gastric ulcer via modulation of ROS/ICAM-1/Nrf2 signaling pathway in Wistar rats |
| title_sort | lactoferrin mitigates ethanol induced gastric ulcer via modulation of ros icam 1 nrf2 signaling pathway in wistar rats |
| topic | anti-oxidant anti-inflammatory ethanol gastric ulcer lactoferrin |
| url | https://ijbms.mums.ac.ir/article_21185_bf7b026b4a0f5f9092c8e074bcb0a07c.pdf |
| work_keys_str_mv | AT gihanasaad lactoferrinmitigatesethanolinducedgastriculcerviamodulationofrosicam1nrf2signalingpathwayinwistarrats AT rashamostafa lactoferrinmitigatesethanolinducedgastriculcerviamodulationofrosicam1nrf2signalingpathwayinwistarrats |