Pulmonary expression of MYCN mRNA following exposure to 2,4-D with or without endotoxin challenge
The present study aimed to observe the expression of MYCN in lungs of mice following chronic exposure of 2,4-D with or without lipopolysaccharide (LPS). 2,4-D was administered orally dissolved in corn oil at high and low dose (1/10th and 1/20th of LD50) for 90 days. After 90 days of exposure, animal...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2019-12-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/95863 |
| _version_ | 1797896730201554944 |
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| author | GEETIKA GEETIKA S S SODHI C S MUKHOPADHYAY RAMNEEK RAMNEEK R S SETHI |
| author_facet | GEETIKA GEETIKA S S SODHI C S MUKHOPADHYAY RAMNEEK RAMNEEK R S SETHI |
| author_sort | GEETIKA GEETIKA |
| collection | DOAJ |
| description | The present study aimed to observe the expression of MYCN in lungs of mice following chronic exposure of 2,4-D with or without lipopolysaccharide (LPS). 2,4-D was administered orally dissolved in corn oil at high and low dose (1/10th and 1/20th of LD50) for 90 days. After 90 days of exposure, animals from each group were challenged with LPS/normal saline solution at 80 μg/animal. The lung tissues were processed for microarray and real time studies. LPS resulted decrease (–0.173 fold) in m-RNA expression level of MYCN as compared to control, while High dose of 2,4-D alone and in combination with LPS resulted 0.949-fold change and 1.656-fold change increase in expression of MYCN m-RNA, respectively, as compared to control. Similarly, Low dose of 2,4-D alone or in combination with LPS also altered MYCN expression. The microarray data when validated by Real Time PCR was found to be in concordance with the Real Time PCR data. The data taken together suggest that, high and low exposure of 2,4-D alone or in combination with LPS alters expression of MYCN at m-RNA level. |
| first_indexed | 2024-04-10T07:47:25Z |
| format | Article |
| id | doaj.art-28c9ea610528409085a1b950167d33d0 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:47:25Z |
| publishDate | 2019-12-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-28c9ea610528409085a1b950167d33d02023-02-23T10:28:22ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272019-12-01891110.56093/ijans.v89i11.95863Pulmonary expression of MYCN mRNA following exposure to 2,4-D with or without endotoxin challengeGEETIKA GEETIKA0S S SODHI1C S MUKHOPADHYAY2RAMNEEK RAMNEEK3R S SETHI4Research Fellow, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 001 IndiaAssistant Professor, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 001 IndiaAssistant Scientist, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 001 IndiaDirector, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 001 IndiaProfessor and Head, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 001 IndiaThe present study aimed to observe the expression of MYCN in lungs of mice following chronic exposure of 2,4-D with or without lipopolysaccharide (LPS). 2,4-D was administered orally dissolved in corn oil at high and low dose (1/10th and 1/20th of LD50) for 90 days. After 90 days of exposure, animals from each group were challenged with LPS/normal saline solution at 80 μg/animal. The lung tissues were processed for microarray and real time studies. LPS resulted decrease (–0.173 fold) in m-RNA expression level of MYCN as compared to control, while High dose of 2,4-D alone and in combination with LPS resulted 0.949-fold change and 1.656-fold change increase in expression of MYCN m-RNA, respectively, as compared to control. Similarly, Low dose of 2,4-D alone or in combination with LPS also altered MYCN expression. The microarray data when validated by Real Time PCR was found to be in concordance with the Real Time PCR data. The data taken together suggest that, high and low exposure of 2,4-D alone or in combination with LPS alters expression of MYCN at m-RNA level.https://epubs.icar.org.in/index.php/IJAnS/article/view/9586324-DMiceMicroarrayMYCNQuantitative real time PCR |
| spellingShingle | GEETIKA GEETIKA S S SODHI C S MUKHOPADHYAY RAMNEEK RAMNEEK R S SETHI Pulmonary expression of MYCN mRNA following exposure to 2,4-D with or without endotoxin challenge Indian Journal of Animal Sciences 2 4-D Mice Microarray MYCN Quantitative real time PCR |
| title | Pulmonary expression of MYCN mRNA following exposure to 2,4-D with or without endotoxin challenge |
| title_full | Pulmonary expression of MYCN mRNA following exposure to 2,4-D with or without endotoxin challenge |
| title_fullStr | Pulmonary expression of MYCN mRNA following exposure to 2,4-D with or without endotoxin challenge |
| title_full_unstemmed | Pulmonary expression of MYCN mRNA following exposure to 2,4-D with or without endotoxin challenge |
| title_short | Pulmonary expression of MYCN mRNA following exposure to 2,4-D with or without endotoxin challenge |
| title_sort | pulmonary expression of mycn mrna following exposure to 2 4 d with or without endotoxin challenge |
| topic | 2 4-D Mice Microarray MYCN Quantitative real time PCR |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/95863 |
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