Development and Analytical Evaluation of a Point-of-Care Electrochemical Biosensor for Rapid and Accurate SARS-CoV-2 Detection
The COVID-19 pandemic has underscored the critical need for rapid and accurate screening and diagnostic methods for potential respiratory viruses. Existing COVID-19 diagnostic approaches face limitations either in terms of turnaround time or accuracy. In this study, we present an electrochemical bio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/18/8000 |
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| author | Mesfin Meshesha Anik Sardar Ruchi Supekar Lopamudra Bhattacharjee Soumyo Chatterjee Nyancy Halder Kallol Mohanta Tarun Kanti Bhattacharyya Biplab Pal |
| author_facet | Mesfin Meshesha Anik Sardar Ruchi Supekar Lopamudra Bhattacharjee Soumyo Chatterjee Nyancy Halder Kallol Mohanta Tarun Kanti Bhattacharyya Biplab Pal |
| author_sort | Mesfin Meshesha |
| collection | DOAJ |
| description | The COVID-19 pandemic has underscored the critical need for rapid and accurate screening and diagnostic methods for potential respiratory viruses. Existing COVID-19 diagnostic approaches face limitations either in terms of turnaround time or accuracy. In this study, we present an electrochemical biosensor that offers nearly instantaneous and precise SARS-CoV-2 detection, suitable for point-of-care and environmental monitoring applications. The biosensor employs a stapled hACE-2 N-terminal alpha helix peptide to functionalize an in situ grown polypyrrole conductive polymer on a nitrocellulose membrane backbone through a chemical process. We assessed the biosensor’s analytical performance using heat-inactivated omicron and delta variants of the SARS-CoV-2 virus in artificial saliva (AS) and nasal swab (NS) samples diluted in a strong ionic solution, as well as clinical specimens with known Ct values. Virus identification was achieved through electrochemical impedance spectroscopy (EIS) and frequency analyses. The assay demonstrated a limit of detection (LoD) of 40 TCID<sub>50</sub>/mL, with 95% sensitivity and 100% specificity. Notably, the biosensor exhibited no cross-reactivity when tested against the influenza virus. The entire testing process using the biosensor takes less than a minute. In summary, our biosensor exhibits promising potential in the battle against pandemic respiratory viruses, offering a platform for the development of rapid, compact, portable, and point-of-care devices capable of multiplexing various viruses. The biosensor has the capacity to significantly bolster our readiness and response to future viral outbreaks. |
| first_indexed | 2024-03-10T22:01:10Z |
| format | Article |
| id | doaj.art-28cc05132b4146e5a1f7c3ef250f254a |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T22:01:10Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-28cc05132b4146e5a1f7c3ef250f254a2023-11-19T12:57:16ZengMDPI AGSensors1424-82202023-09-012318800010.3390/s23188000Development and Analytical Evaluation of a Point-of-Care Electrochemical Biosensor for Rapid and Accurate SARS-CoV-2 DetectionMesfin Meshesha0Anik Sardar1Ruchi Supekar2Lopamudra Bhattacharjee3Soumyo Chatterjee4Nyancy Halder5Kallol Mohanta6Tarun Kanti Bhattacharyya7Biplab Pal8Department of Virology, Opteev Technologies Inc., Baltimore, MD 21225, USAResearch and Development Laboratory, Opteev Healthtech, GN-4, Sector-V, Kolkata 700091, IndiaResearch and Development Laboratory, Opteev Healthtech, GN-4, Sector-V, Kolkata 700091, IndiaResearch and Development Laboratory, Opteev Healthtech, GN-4, Sector-V, Kolkata 700091, IndiaResearch and Development Laboratory, Opteev Healthtech, GN-4, Sector-V, Kolkata 700091, IndiaResearch and Development Laboratory, Opteev Healthtech, GN-4, Sector-V, Kolkata 700091, IndiaResearch and Development Laboratory, Opteev Healthtech, GN-4, Sector-V, Kolkata 700091, IndiaDepartment of Electronics and Electrical Communication Engineering, Indian Institute of Technology, Kharagpur 721302, IndiaDepartment of Virology, Opteev Technologies Inc., Baltimore, MD 21225, USAThe COVID-19 pandemic has underscored the critical need for rapid and accurate screening and diagnostic methods for potential respiratory viruses. Existing COVID-19 diagnostic approaches face limitations either in terms of turnaround time or accuracy. In this study, we present an electrochemical biosensor that offers nearly instantaneous and precise SARS-CoV-2 detection, suitable for point-of-care and environmental monitoring applications. The biosensor employs a stapled hACE-2 N-terminal alpha helix peptide to functionalize an in situ grown polypyrrole conductive polymer on a nitrocellulose membrane backbone through a chemical process. We assessed the biosensor’s analytical performance using heat-inactivated omicron and delta variants of the SARS-CoV-2 virus in artificial saliva (AS) and nasal swab (NS) samples diluted in a strong ionic solution, as well as clinical specimens with known Ct values. Virus identification was achieved through electrochemical impedance spectroscopy (EIS) and frequency analyses. The assay demonstrated a limit of detection (LoD) of 40 TCID<sub>50</sub>/mL, with 95% sensitivity and 100% specificity. Notably, the biosensor exhibited no cross-reactivity when tested against the influenza virus. The entire testing process using the biosensor takes less than a minute. In summary, our biosensor exhibits promising potential in the battle against pandemic respiratory viruses, offering a platform for the development of rapid, compact, portable, and point-of-care devices capable of multiplexing various viruses. The biosensor has the capacity to significantly bolster our readiness and response to future viral outbreaks.https://www.mdpi.com/1424-8220/23/18/8000SARS-CoV-2electrochemical biosensorlactam-stapled peptideimpedance spectroscopy |
| spellingShingle | Mesfin Meshesha Anik Sardar Ruchi Supekar Lopamudra Bhattacharjee Soumyo Chatterjee Nyancy Halder Kallol Mohanta Tarun Kanti Bhattacharyya Biplab Pal Development and Analytical Evaluation of a Point-of-Care Electrochemical Biosensor for Rapid and Accurate SARS-CoV-2 Detection Sensors SARS-CoV-2 electrochemical biosensor lactam-stapled peptide impedance spectroscopy |
| title | Development and Analytical Evaluation of a Point-of-Care Electrochemical Biosensor for Rapid and Accurate SARS-CoV-2 Detection |
| title_full | Development and Analytical Evaluation of a Point-of-Care Electrochemical Biosensor for Rapid and Accurate SARS-CoV-2 Detection |
| title_fullStr | Development and Analytical Evaluation of a Point-of-Care Electrochemical Biosensor for Rapid and Accurate SARS-CoV-2 Detection |
| title_full_unstemmed | Development and Analytical Evaluation of a Point-of-Care Electrochemical Biosensor for Rapid and Accurate SARS-CoV-2 Detection |
| title_short | Development and Analytical Evaluation of a Point-of-Care Electrochemical Biosensor for Rapid and Accurate SARS-CoV-2 Detection |
| title_sort | development and analytical evaluation of a point of care electrochemical biosensor for rapid and accurate sars cov 2 detection |
| topic | SARS-CoV-2 electrochemical biosensor lactam-stapled peptide impedance spectroscopy |
| url | https://www.mdpi.com/1424-8220/23/18/8000 |
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