Comparison of Cost and Potency of Human Mesenchymal Stromal Cell Conditioned Medium Derived from 2- and 3-Dimensional Cultures
Mesenchymal stromal cell (MSC)-derived products, such as trophic factors (MTFs), have anti-inflammatory properties that make them attractive for cell-free treatment. Three-dimensional (3D) culture can enhance these properties, and large-scale expansion using a bioreactor can reduce manufacturing cos...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/8/930 |
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| author | Marialaura Madrigal Patricia L. Fernández Ricardo Lleonart Lizmar Carreño Kaiser Alejandro Villalobos Gorday Ellís Rodríguez Kathya de Cupeiro Carlos M. Restrepo K. S. Jagannatha Rao Neil H. Riordan |
| author_facet | Marialaura Madrigal Patricia L. Fernández Ricardo Lleonart Lizmar Carreño Kaiser Alejandro Villalobos Gorday Ellís Rodríguez Kathya de Cupeiro Carlos M. Restrepo K. S. Jagannatha Rao Neil H. Riordan |
| author_sort | Marialaura Madrigal |
| collection | DOAJ |
| description | Mesenchymal stromal cell (MSC)-derived products, such as trophic factors (MTFs), have anti-inflammatory properties that make them attractive for cell-free treatment. Three-dimensional (3D) culture can enhance these properties, and large-scale expansion using a bioreactor can reduce manufacturing costs. Three lots of MTFs were obtained from umbilical cord MSCs produced by either monolayer culture (Monol MTF) or using a 3D microcarrier in a spinner flask dynamic system (Bioreactor MTF). The resulting MTFs were tested and compared using anti-inflammatory potency assays in two different systems: (1) a phytohemagglutinin-activated peripheral blood mononuclear cell (PBMNC) system and (2) a lipopolysaccharide (LPS)-activated macrophage system. Cytokine expression by macrophages was measured via RT-PCR. The production costs of hypothetical units of anti-inflammatory effects were calculated using the percentage of TNF-α inhibition by MTF exposure. Bioreactor MTFs had a higher inhibitory effect on TNF (<i>p</i> < 0.01) than monolayer MTFs (<i>p</i> < 0.05). The anti-inflammatory effect of Bioreactor MTFs on IL-1β, TNF-α, IL-8, IL-6, and MIP-1 was significantly higher than that of monolayer MTFs. The production cost of 1% inhibition of TNF-α was 11–40% higher using monolayer culture compared to bioreactor-derived MTFs. A 3D dynamic culture was, therefore, able to produce high-quality MTFs, with robust anti-inflammatory properties, more efficiently than monolayer static systems. |
| first_indexed | 2024-03-11T00:06:09Z |
| format | Article |
| id | doaj.art-28d0c7a1851d4715b333f5509b0007f4 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T00:06:09Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-28d0c7a1851d4715b333f5509b0007f42023-11-19T00:18:00ZengMDPI AGBioengineering2306-53542023-08-0110893010.3390/bioengineering10080930Comparison of Cost and Potency of Human Mesenchymal Stromal Cell Conditioned Medium Derived from 2- and 3-Dimensional CulturesMarialaura Madrigal0Patricia L. Fernández1Ricardo Lleonart2Lizmar Carreño3Kaiser Alejandro Villalobos Gorday4Ellís Rodríguez5Kathya de Cupeiro6Carlos M. Restrepo7K. S. Jagannatha Rao8Neil H. Riordan9MediStem Panama Inc., Panama City 7144, PanamaCentro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), Panama City 7144, PanamaCentro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), Panama City 7144, PanamaMediStem Panama Inc., Panama City 7144, PanamaMediStem Panama Inc., Panama City 7144, PanamaMediStem Panama Inc., Panama City 7144, PanamaMediStem Panama Inc., Panama City 7144, PanamaCentro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), Panama City 7144, PanamaDepartment of Biotechnology, Konenru Lakshmaiah Education Foundation (KLEF) deemed to be University, Vaddeswaram 522302, IndiaMediStem Panama Inc., Panama City 7144, PanamaMesenchymal stromal cell (MSC)-derived products, such as trophic factors (MTFs), have anti-inflammatory properties that make them attractive for cell-free treatment. Three-dimensional (3D) culture can enhance these properties, and large-scale expansion using a bioreactor can reduce manufacturing costs. Three lots of MTFs were obtained from umbilical cord MSCs produced by either monolayer culture (Monol MTF) or using a 3D microcarrier in a spinner flask dynamic system (Bioreactor MTF). The resulting MTFs were tested and compared using anti-inflammatory potency assays in two different systems: (1) a phytohemagglutinin-activated peripheral blood mononuclear cell (PBMNC) system and (2) a lipopolysaccharide (LPS)-activated macrophage system. Cytokine expression by macrophages was measured via RT-PCR. The production costs of hypothetical units of anti-inflammatory effects were calculated using the percentage of TNF-α inhibition by MTF exposure. Bioreactor MTFs had a higher inhibitory effect on TNF (<i>p</i> < 0.01) than monolayer MTFs (<i>p</i> < 0.05). The anti-inflammatory effect of Bioreactor MTFs on IL-1β, TNF-α, IL-8, IL-6, and MIP-1 was significantly higher than that of monolayer MTFs. The production cost of 1% inhibition of TNF-α was 11–40% higher using monolayer culture compared to bioreactor-derived MTFs. A 3D dynamic culture was, therefore, able to produce high-quality MTFs, with robust anti-inflammatory properties, more efficiently than monolayer static systems.https://www.mdpi.com/2306-5354/10/8/930UC-MSCtrophic factorsconditioned mediumtridimensional culturebioreactormesenchymal |
| spellingShingle | Marialaura Madrigal Patricia L. Fernández Ricardo Lleonart Lizmar Carreño Kaiser Alejandro Villalobos Gorday Ellís Rodríguez Kathya de Cupeiro Carlos M. Restrepo K. S. Jagannatha Rao Neil H. Riordan Comparison of Cost and Potency of Human Mesenchymal Stromal Cell Conditioned Medium Derived from 2- and 3-Dimensional Cultures Bioengineering UC-MSC trophic factors conditioned medium tridimensional culture bioreactor mesenchymal |
| title | Comparison of Cost and Potency of Human Mesenchymal Stromal Cell Conditioned Medium Derived from 2- and 3-Dimensional Cultures |
| title_full | Comparison of Cost and Potency of Human Mesenchymal Stromal Cell Conditioned Medium Derived from 2- and 3-Dimensional Cultures |
| title_fullStr | Comparison of Cost and Potency of Human Mesenchymal Stromal Cell Conditioned Medium Derived from 2- and 3-Dimensional Cultures |
| title_full_unstemmed | Comparison of Cost and Potency of Human Mesenchymal Stromal Cell Conditioned Medium Derived from 2- and 3-Dimensional Cultures |
| title_short | Comparison of Cost and Potency of Human Mesenchymal Stromal Cell Conditioned Medium Derived from 2- and 3-Dimensional Cultures |
| title_sort | comparison of cost and potency of human mesenchymal stromal cell conditioned medium derived from 2 and 3 dimensional cultures |
| topic | UC-MSC trophic factors conditioned medium tridimensional culture bioreactor mesenchymal |
| url | https://www.mdpi.com/2306-5354/10/8/930 |
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