Association of LGR5 Expression with Gastric Cancer and Helicobacter pylori CagA Genotype
Background and purpose: Given the role of cancer stem cells in cancer, the aim of this study was to determine the expression of LGR5 marker in gastric cancer and its association with cagA genotype of Helicobacter pylori infection. Materials and methods: A case-control study was performed in gastric...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences
2021-05-01
|
| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-16580-en.html |
| _version_ | 1797943984518070272 |
|---|---|
| author | Fatemeh Montazer Hossein Lamsehchi Reza Valadan Akbar Hedayatizadeh-Omran Mohammad Eslamijouybari Ghasem Janbabai Omolbanin Amjadi Mohadeseh Ahmadi Reza Alizadeh-Navaei |
| author_facet | Fatemeh Montazer Hossein Lamsehchi Reza Valadan Akbar Hedayatizadeh-Omran Mohammad Eslamijouybari Ghasem Janbabai Omolbanin Amjadi Mohadeseh Ahmadi Reza Alizadeh-Navaei |
| author_sort | Fatemeh Montazer |
| collection | DOAJ |
| description | Background and purpose: Given the role of cancer stem cells in cancer, the aim of this study was to determine the expression of LGR5 marker in gastric cancer and its association with cagA genotype of Helicobacter pylori infection.
Materials and methods: A case-control study was performed in gastric biopsy specimens from antrum and body during endoscopic examination of patients attending Sari Touba Clinic, 2017-2018. After reviewing the patient records, the samples from those aged 50 and higher were studied. Case group included gastric cancer specimens with H. pylori infection (n=30) and control group included non-cancerous samples with H. pylori infection (n=30). LGR5 expression and presence of cagA were evaluated by IHC and PCR methods, respectively.
Results: The mean ages of gastric cancer and control group were 69.5±10.1 and 62.3±7.8, respectively (P= 0.003). Twenty three patients (76.7%) in cancer group and 24 patients (80%) in control group were positive for cagA genotype. Overexpression of LGR5 was observed in 15 patients (51.7%) with gastric cancer and 11 patients (39.3%) in control group (P= 0.429). LGR5 was also overexpressed in 18 cases (40.9%) with cagA positive genotype and 8 cases (61.5%) with negative cagA genotype (P=0.22).
Conclusion: High expression of LGR5 was observed in half of patients with gastric cancer but it was not significantly associated with cagA H.pylori genotype. |
| first_indexed | 2024-04-10T20:32:34Z |
| format | Article |
| id | doaj.art-28d21d6b899b4cc58028e8595766eea7 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:32:34Z |
| publishDate | 2021-05-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-28d21d6b899b4cc58028e8595766eea72023-01-25T05:14:14ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792021-05-013119793100Association of LGR5 Expression with Gastric Cancer and Helicobacter pylori CagA GenotypeFatemeh Montazer0Hossein Lamsehchi1Reza Valadan2Akbar Hedayatizadeh-Omran3Mohammad Eslamijouybari4Ghasem Janbabai5Omolbanin Amjadi6Mohadeseh Ahmadi7Reza Alizadeh-Navaei8 Assistant Professor, Department of Pathology, Iran University of Medical Sciences, Tehran, Iran General Practitioner, Mazandaran University of Medical Sciences, Sari, Iran Assistant Professor, Department of Immunology, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran Associate Professor, Gastrointestinal Cancer Research Center, Non-communicable Diseases Institute ,Mazandaran University of Medical Sciences, Sari, Iran Assistant Professor, Department of Internal Medicine, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Professor, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran MSc in Molecular and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran MSc in Biotechnology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Assistant Professor, Gastrointestinal Cancer Research Center, Non-communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran Background and purpose: Given the role of cancer stem cells in cancer, the aim of this study was to determine the expression of LGR5 marker in gastric cancer and its association with cagA genotype of Helicobacter pylori infection. Materials and methods: A case-control study was performed in gastric biopsy specimens from antrum and body during endoscopic examination of patients attending Sari Touba Clinic, 2017-2018. After reviewing the patient records, the samples from those aged 50 and higher were studied. Case group included gastric cancer specimens with H. pylori infection (n=30) and control group included non-cancerous samples with H. pylori infection (n=30). LGR5 expression and presence of cagA were evaluated by IHC and PCR methods, respectively. Results: The mean ages of gastric cancer and control group were 69.5±10.1 and 62.3±7.8, respectively (P= 0.003). Twenty three patients (76.7%) in cancer group and 24 patients (80%) in control group were positive for cagA genotype. Overexpression of LGR5 was observed in 15 patients (51.7%) with gastric cancer and 11 patients (39.3%) in control group (P= 0.429). LGR5 was also overexpressed in 18 cases (40.9%) with cagA positive genotype and 8 cases (61.5%) with negative cagA genotype (P=0.22). Conclusion: High expression of LGR5 was observed in half of patients with gastric cancer but it was not significantly associated with cagA H.pylori genotype.http://jmums.mazums.ac.ir/article-1-16580-en.htmlgastric cancerhelicobacter pylorilgr5 |
| spellingShingle | Fatemeh Montazer Hossein Lamsehchi Reza Valadan Akbar Hedayatizadeh-Omran Mohammad Eslamijouybari Ghasem Janbabai Omolbanin Amjadi Mohadeseh Ahmadi Reza Alizadeh-Navaei Association of LGR5 Expression with Gastric Cancer and Helicobacter pylori CagA Genotype Journal of Mazandaran University of Medical Sciences gastric cancer helicobacter pylori lgr5 |
| title | Association of LGR5 Expression with Gastric Cancer and Helicobacter pylori CagA Genotype |
| title_full | Association of LGR5 Expression with Gastric Cancer and Helicobacter pylori CagA Genotype |
| title_fullStr | Association of LGR5 Expression with Gastric Cancer and Helicobacter pylori CagA Genotype |
| title_full_unstemmed | Association of LGR5 Expression with Gastric Cancer and Helicobacter pylori CagA Genotype |
| title_short | Association of LGR5 Expression with Gastric Cancer and Helicobacter pylori CagA Genotype |
| title_sort | association of lgr5 expression with gastric cancer and helicobacter pylori caga genotype |
| topic | gastric cancer helicobacter pylori lgr5 |
| url | http://jmums.mazums.ac.ir/article-1-16580-en.html |
| work_keys_str_mv | AT fatemehmontazer associationoflgr5expressionwithgastriccancerandhelicobacterpyloricagagenotype AT hosseinlamsehchi associationoflgr5expressionwithgastriccancerandhelicobacterpyloricagagenotype AT rezavaladan associationoflgr5expressionwithgastriccancerandhelicobacterpyloricagagenotype AT akbarhedayatizadehomran associationoflgr5expressionwithgastriccancerandhelicobacterpyloricagagenotype AT mohammadeslamijouybari associationoflgr5expressionwithgastriccancerandhelicobacterpyloricagagenotype AT ghasemjanbabai associationoflgr5expressionwithgastriccancerandhelicobacterpyloricagagenotype AT omolbaninamjadi associationoflgr5expressionwithgastriccancerandhelicobacterpyloricagagenotype AT mohadesehahmadi associationoflgr5expressionwithgastriccancerandhelicobacterpyloricagagenotype AT rezaalizadehnavaei associationoflgr5expressionwithgastriccancerandhelicobacterpyloricagagenotype |