Coix lacryma-jobi var. ma-yuen Stapf sprout extract has anti-metastatic activity in colon cancer cells in vitro
Abstract Background Coix lacryma-jobi var. ma-yuen (Rom.Caill.) Stapf has been used in China as an herbal medicine. Many studies of this plant have reported anti-proliferative and apoptotic activities on human cancer cell lines. Therefore, this study of the anti-metastatic effect of Coix lacryma-job...
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-017-1990-y |
| _version_ | 1818576538778468352 |
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| author | Eun Suk Son Young Ock Kim Chun Geon Park Kyung Hun Park Sung Hwan Jeong Jeong-Woong Park Se-Hee Kim |
| author_facet | Eun Suk Son Young Ock Kim Chun Geon Park Kyung Hun Park Sung Hwan Jeong Jeong-Woong Park Se-Hee Kim |
| author_sort | Eun Suk Son |
| collection | DOAJ |
| description | Abstract Background Coix lacryma-jobi var. ma-yuen (Rom.Caill.) Stapf has been used in China as an herbal medicine. Many studies of this plant have reported anti-proliferative and apoptotic activities on human cancer cell lines. Therefore, this study of the anti-metastatic effect of Coix lacryma-jobi var. ma-yuen Stapf sprout extract (CLSE) in colorectal cancer cells may provide a scientific basis for exploring anti-cancer effects of edible crops. Methods To evaluate the effect of CLSE on cell proliferation and signaling, we performed a Cell Counting Kit-8 (CCK-8) assay in HCT116 cells and used western blot analysis. Furthermore, scratch-wound healing, transwell migration, matrigel invasion, and adhesion assays were conducted to elucidate the anti-metastatic effects of CLSE under hypoxic conditions in colon cancer cells. Results First, CLSE decreased deferoxamine (DFO)-induced migration of colon cancer cells by 87%, and blocked colon cancer cell migration by 80% compared with hypoxia control cells. Second, CLSE treatment resulted in a 54% reduction in hypoxia-induced invasiveness of colon cancer cells, and 50% inhibition of adhesive potency through inactivation of the extracellular signal-regulated kinase (ERK) 1/2 and protein kinase b (AKT) pathways. Third, conditioned medium collected from CLSE-treated HCT116 cells suppressed tube formation of human umbilical vein endothelial cells (HUVECs) by 91%. Conclusions CLSE inhibited migration, invasion, and adhesion of colon cancer cells and tube formation by HUVECs via repression of the ERK1/2 and AKT pathways under hypoxic conditions. Therefore, CLSE may be used to treat patients with colon cancer. |
| first_indexed | 2024-12-16T06:15:37Z |
| format | Article |
| id | doaj.art-28d34c9cd0504982ab4df3fa899052c7 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-16T06:15:37Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-28d34c9cd0504982ab4df3fa899052c72022-12-21T22:41:15ZengBMCBMC Complementary and Alternative Medicine1472-68822017-11-011711910.1186/s12906-017-1990-yCoix lacryma-jobi var. ma-yuen Stapf sprout extract has anti-metastatic activity in colon cancer cells in vitroEun Suk Son0Young Ock Kim1Chun Geon Park2Kyung Hun Park3Sung Hwan Jeong4Jeong-Woong Park5Se-Hee Kim6Department of Internal Medicine, Gachon University Gil Medical CenterDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDADepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDADepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDADepartment of Internal Medicine, Gachon University Gil Medical CenterDepartment of Internal Medicine, Gachon University Gil Medical CenterGachon medical research institute, Gachon University Gil Medical CenterAbstract Background Coix lacryma-jobi var. ma-yuen (Rom.Caill.) Stapf has been used in China as an herbal medicine. Many studies of this plant have reported anti-proliferative and apoptotic activities on human cancer cell lines. Therefore, this study of the anti-metastatic effect of Coix lacryma-jobi var. ma-yuen Stapf sprout extract (CLSE) in colorectal cancer cells may provide a scientific basis for exploring anti-cancer effects of edible crops. Methods To evaluate the effect of CLSE on cell proliferation and signaling, we performed a Cell Counting Kit-8 (CCK-8) assay in HCT116 cells and used western blot analysis. Furthermore, scratch-wound healing, transwell migration, matrigel invasion, and adhesion assays were conducted to elucidate the anti-metastatic effects of CLSE under hypoxic conditions in colon cancer cells. Results First, CLSE decreased deferoxamine (DFO)-induced migration of colon cancer cells by 87%, and blocked colon cancer cell migration by 80% compared with hypoxia control cells. Second, CLSE treatment resulted in a 54% reduction in hypoxia-induced invasiveness of colon cancer cells, and 50% inhibition of adhesive potency through inactivation of the extracellular signal-regulated kinase (ERK) 1/2 and protein kinase b (AKT) pathways. Third, conditioned medium collected from CLSE-treated HCT116 cells suppressed tube formation of human umbilical vein endothelial cells (HUVECs) by 91%. Conclusions CLSE inhibited migration, invasion, and adhesion of colon cancer cells and tube formation by HUVECs via repression of the ERK1/2 and AKT pathways under hypoxic conditions. Therefore, CLSE may be used to treat patients with colon cancer.http://link.springer.com/article/10.1186/s12906-017-1990-yCoixColon cancerMetastasisInvasionHypoxia |
| spellingShingle | Eun Suk Son Young Ock Kim Chun Geon Park Kyung Hun Park Sung Hwan Jeong Jeong-Woong Park Se-Hee Kim Coix lacryma-jobi var. ma-yuen Stapf sprout extract has anti-metastatic activity in colon cancer cells in vitro BMC Complementary and Alternative Medicine Coix Colon cancer Metastasis Invasion Hypoxia |
| title | Coix lacryma-jobi var. ma-yuen Stapf sprout extract has anti-metastatic activity in colon cancer cells in vitro |
| title_full | Coix lacryma-jobi var. ma-yuen Stapf sprout extract has anti-metastatic activity in colon cancer cells in vitro |
| title_fullStr | Coix lacryma-jobi var. ma-yuen Stapf sprout extract has anti-metastatic activity in colon cancer cells in vitro |
| title_full_unstemmed | Coix lacryma-jobi var. ma-yuen Stapf sprout extract has anti-metastatic activity in colon cancer cells in vitro |
| title_short | Coix lacryma-jobi var. ma-yuen Stapf sprout extract has anti-metastatic activity in colon cancer cells in vitro |
| title_sort | coix lacryma jobi var ma yuen stapf sprout extract has anti metastatic activity in colon cancer cells in vitro |
| topic | Coix Colon cancer Metastasis Invasion Hypoxia |
| url | http://link.springer.com/article/10.1186/s12906-017-1990-y |
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