Anopheline and culicine mosquitoes are not repelled by surfaces treated with the entomopathogenic fungi <it>Metarhizium anisopliae </it>and <it>Beauveria bassiana</it>
<p>Abstract</p> <p>Background</p> <p>Entomopathogenic fungi, <it>Metarhizium anisopliae </it>and <it>Beauveria bassiana</it>, are promising bio-pesticides for application against adult malaria mosquito vectors. An understanding of the behavioural...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-08-01
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| Series: | Parasites & Vectors |
| Online Access: | http://www.parasitesandvectors.com/content/3/1/80 |
| _version_ | 1818255755946491904 |
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| author | Mnyone Ladslaus L Koenraadt Constantianus JM Lyimo Issa N Mpingwa Monica W Takken Willem Russell Tanya L |
| author_facet | Mnyone Ladslaus L Koenraadt Constantianus JM Lyimo Issa N Mpingwa Monica W Takken Willem Russell Tanya L |
| author_sort | Mnyone Ladslaus L |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Entomopathogenic fungi, <it>Metarhizium anisopliae </it>and <it>Beauveria bassiana</it>, are promising bio-pesticides for application against adult malaria mosquito vectors. An understanding of the behavioural responses of mosquitoes towards these fungi is necessary to guide development of fungi beyond the 'proof of concept' stage and to design suitable intervention tools.</p> <p>Methods</p> <p>Here we tested whether oil-formulations of the two fungi could be detected and avoided by adult <it>Anopheles gambiae s.s., Anopheles arabiensis </it>and <it>Culex quinquefasciatus</it>. The bioassays used a glass chamber divided into three compartments (each 250 × 250 × 250 mm): release, middle and stimulus compartments. Netting with or without fungus was fitted in front of the stimulus compartment. Mosquitoes were released and the proportion that entered the stimulus compartment was determined and compared between treatments. Treatments were untreated netting (control 1), netting with mineral oil (control 2) and fungal conidia formulated in mineral oil evaluated at three different dosages (2 × 10<sup>10</sup>, 4 × 10<sup>10 </sup>and 8 × 10<sup>10 </sup>conidia m<sup>-2</sup>).</p> <p>Results</p> <p>Neither fungal strain was repellent as the mean proportion of mosquitoes collected in the stimulus compartment did not differ between experiments with surfaces treated with and without fungus regardless of the fungal isolate and mosquito species tested.</p> <p>Conclusion</p> <p>Our results indicate that mineral-oil formulations of <it>M. anisopliae </it>and <it>B. bassiana </it>were not repellent against the mosquito species tested. Therefore, both fungi are suitable candidates for the further development of tools that aim to control host-seeking or resting mosquitoes using entomopathogenic fungi.</p> |
| first_indexed | 2024-12-12T17:16:55Z |
| format | Article |
| id | doaj.art-28d4676f58db4a3dbd904c3cc9594887 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-12T17:16:55Z |
| publishDate | 2010-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-28d4676f58db4a3dbd904c3cc95948872022-12-22T00:17:45ZengBMCParasites & Vectors1756-33052010-08-01318010.1186/1756-3305-3-80Anopheline and culicine mosquitoes are not repelled by surfaces treated with the entomopathogenic fungi <it>Metarhizium anisopliae </it>and <it>Beauveria bassiana</it>Mnyone Ladslaus LKoenraadt Constantianus JMLyimo Issa NMpingwa Monica WTakken WillemRussell Tanya L<p>Abstract</p> <p>Background</p> <p>Entomopathogenic fungi, <it>Metarhizium anisopliae </it>and <it>Beauveria bassiana</it>, are promising bio-pesticides for application against adult malaria mosquito vectors. An understanding of the behavioural responses of mosquitoes towards these fungi is necessary to guide development of fungi beyond the 'proof of concept' stage and to design suitable intervention tools.</p> <p>Methods</p> <p>Here we tested whether oil-formulations of the two fungi could be detected and avoided by adult <it>Anopheles gambiae s.s., Anopheles arabiensis </it>and <it>Culex quinquefasciatus</it>. The bioassays used a glass chamber divided into three compartments (each 250 × 250 × 250 mm): release, middle and stimulus compartments. Netting with or without fungus was fitted in front of the stimulus compartment. Mosquitoes were released and the proportion that entered the stimulus compartment was determined and compared between treatments. Treatments were untreated netting (control 1), netting with mineral oil (control 2) and fungal conidia formulated in mineral oil evaluated at three different dosages (2 × 10<sup>10</sup>, 4 × 10<sup>10 </sup>and 8 × 10<sup>10 </sup>conidia m<sup>-2</sup>).</p> <p>Results</p> <p>Neither fungal strain was repellent as the mean proportion of mosquitoes collected in the stimulus compartment did not differ between experiments with surfaces treated with and without fungus regardless of the fungal isolate and mosquito species tested.</p> <p>Conclusion</p> <p>Our results indicate that mineral-oil formulations of <it>M. anisopliae </it>and <it>B. bassiana </it>were not repellent against the mosquito species tested. Therefore, both fungi are suitable candidates for the further development of tools that aim to control host-seeking or resting mosquitoes using entomopathogenic fungi.</p>http://www.parasitesandvectors.com/content/3/1/80 |
| spellingShingle | Mnyone Ladslaus L Koenraadt Constantianus JM Lyimo Issa N Mpingwa Monica W Takken Willem Russell Tanya L Anopheline and culicine mosquitoes are not repelled by surfaces treated with the entomopathogenic fungi <it>Metarhizium anisopliae </it>and <it>Beauveria bassiana</it> Parasites & Vectors |
| title | Anopheline and culicine mosquitoes are not repelled by surfaces treated with the entomopathogenic fungi <it>Metarhizium anisopliae </it>and <it>Beauveria bassiana</it> |
| title_full | Anopheline and culicine mosquitoes are not repelled by surfaces treated with the entomopathogenic fungi <it>Metarhizium anisopliae </it>and <it>Beauveria bassiana</it> |
| title_fullStr | Anopheline and culicine mosquitoes are not repelled by surfaces treated with the entomopathogenic fungi <it>Metarhizium anisopliae </it>and <it>Beauveria bassiana</it> |
| title_full_unstemmed | Anopheline and culicine mosquitoes are not repelled by surfaces treated with the entomopathogenic fungi <it>Metarhizium anisopliae </it>and <it>Beauveria bassiana</it> |
| title_short | Anopheline and culicine mosquitoes are not repelled by surfaces treated with the entomopathogenic fungi <it>Metarhizium anisopliae </it>and <it>Beauveria bassiana</it> |
| title_sort | anopheline and culicine mosquitoes are not repelled by surfaces treated with the entomopathogenic fungi it metarhizium anisopliae it and it beauveria bassiana it |
| url | http://www.parasitesandvectors.com/content/3/1/80 |
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