Sperm fine-needle aspiration (FNA) mapping after failed microdissection testicular sperm extraction (TESE): location and patterns of found sperm
We sought to evaluate the ability of fine-needle aspiration (FNA) mapping to find sperm and to guide sperm retrieval after failed microdissection testicular sperm extraction (micro-TESE) in nonobstructive azoospermic men. In this study of consecutive male infertility cases, interventions included te...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2019-01-01
|
| Series: | Asian Journal of Andrology |
| Subjects: | |
| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2019;volume=21;issue=1;spage=50;epage=55;aulast=Jarvis |
| _version_ | 1819096688469475328 |
|---|---|
| author | Sheba Jarvis Heather K Yee Natalia Thomas Imok Cha Kedar Che Prasad Jonathan W A Ramsay Paul J Turek |
| author_facet | Sheba Jarvis Heather K Yee Natalia Thomas Imok Cha Kedar Che Prasad Jonathan W A Ramsay Paul J Turek |
| author_sort | Sheba Jarvis |
| collection | DOAJ |
| description | We sought to evaluate the ability of fine-needle aspiration (FNA) mapping to find sperm and to guide sperm retrieval after failed microdissection testicular sperm extraction (micro-TESE) in nonobstructive azoospermic men. In this study of consecutive male infertility cases, interventions included testicular FNA mapping and subsequent sperm retrieval. Outcomes included the frequency and location of found sperm on FNA maps after failed micro-TESE and the salvage sperm retrieval success. Among 548 patients undergoing FNA mapping from 2010 to 2016, 82 men with previous micro-TESE procedures were identified. The mean time between micro-TESE and FNA mapping was 2.2 years. A total of 2825 (1424 on right and 1401 on left) sites were mapped. At least one site revealed mature sperm in 24 (29.3%) of 82 men with prior failed micro-TESE procedures. There was an equal likelihood of detecting sperm in either testis (6.1% right; 5.7% left; P = 0.58). Digital “heat maps” revealed differences in sperm findings within the testis with mature sperm more likely found in the testis periphery rather than centrally. Fifteen (62.5%) patients subsequently underwent sperm retrieval procedures guided by FNA maps. Sufficient sperm were retrieved in all cases, and in 10 (66.7%) of 15 cases, extra sperm were frozen for future use. In a significant proportion of failed micro-TESE procedures representing the largest study to date, sperm were detected by FNA mapping and could be reliably retrieved through FNA map-guided surgical sperm retrieval. When present, sperm were more likely to be found in the testis periphery rather than centrally with FNA mapping. |
| first_indexed | 2024-12-22T00:03:10Z |
| format | Article |
| id | doaj.art-28d76915b1bf439cbd16f99d762e23a2 |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-12-22T00:03:10Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-28d76915b1bf439cbd16f99d762e23a22022-12-21T18:45:37ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622019-01-01211505510.4103/aja.aja_68_18Sperm fine-needle aspiration (FNA) mapping after failed microdissection testicular sperm extraction (TESE): location and patterns of found spermSheba JarvisHeather K YeeNatalia ThomasImok ChaKedar Che PrasadJonathan W A RamsayPaul J TurekWe sought to evaluate the ability of fine-needle aspiration (FNA) mapping to find sperm and to guide sperm retrieval after failed microdissection testicular sperm extraction (micro-TESE) in nonobstructive azoospermic men. In this study of consecutive male infertility cases, interventions included testicular FNA mapping and subsequent sperm retrieval. Outcomes included the frequency and location of found sperm on FNA maps after failed micro-TESE and the salvage sperm retrieval success. Among 548 patients undergoing FNA mapping from 2010 to 2016, 82 men with previous micro-TESE procedures were identified. The mean time between micro-TESE and FNA mapping was 2.2 years. A total of 2825 (1424 on right and 1401 on left) sites were mapped. At least one site revealed mature sperm in 24 (29.3%) of 82 men with prior failed micro-TESE procedures. There was an equal likelihood of detecting sperm in either testis (6.1% right; 5.7% left; P = 0.58). Digital “heat maps” revealed differences in sperm findings within the testis with mature sperm more likely found in the testis periphery rather than centrally. Fifteen (62.5%) patients subsequently underwent sperm retrieval procedures guided by FNA maps. Sufficient sperm were retrieved in all cases, and in 10 (66.7%) of 15 cases, extra sperm were frozen for future use. In a significant proportion of failed micro-TESE procedures representing the largest study to date, sperm were detected by FNA mapping and could be reliably retrieved through FNA map-guided surgical sperm retrieval. When present, sperm were more likely to be found in the testis periphery rather than centrally with FNA mapping.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2019;volume=21;issue=1;spage=50;epage=55;aulast=Jarvisazoospermia; hypogonadism;IVF-ICSI; microdissection TESE; sperm FNA mapping; sperm retrieval |
| spellingShingle | Sheba Jarvis Heather K Yee Natalia Thomas Imok Cha Kedar Che Prasad Jonathan W A Ramsay Paul J Turek Sperm fine-needle aspiration (FNA) mapping after failed microdissection testicular sperm extraction (TESE): location and patterns of found sperm Asian Journal of Andrology azoospermia; hypogonadism;IVF-ICSI; microdissection TESE; sperm FNA mapping; sperm retrieval |
| title | Sperm fine-needle aspiration (FNA) mapping after failed microdissection testicular sperm extraction (TESE): location and patterns of found sperm |
| title_full | Sperm fine-needle aspiration (FNA) mapping after failed microdissection testicular sperm extraction (TESE): location and patterns of found sperm |
| title_fullStr | Sperm fine-needle aspiration (FNA) mapping after failed microdissection testicular sperm extraction (TESE): location and patterns of found sperm |
| title_full_unstemmed | Sperm fine-needle aspiration (FNA) mapping after failed microdissection testicular sperm extraction (TESE): location and patterns of found sperm |
| title_short | Sperm fine-needle aspiration (FNA) mapping after failed microdissection testicular sperm extraction (TESE): location and patterns of found sperm |
| title_sort | sperm fine needle aspiration fna mapping after failed microdissection testicular sperm extraction tese location and patterns of found sperm |
| topic | azoospermia; hypogonadism;IVF-ICSI; microdissection TESE; sperm FNA mapping; sperm retrieval |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2019;volume=21;issue=1;spage=50;epage=55;aulast=Jarvis |
| work_keys_str_mv | AT shebajarvis spermfineneedleaspirationfnamappingafterfailedmicrodissectiontesticularspermextractionteselocationandpatternsoffoundsperm AT heatherkyee spermfineneedleaspirationfnamappingafterfailedmicrodissectiontesticularspermextractionteselocationandpatternsoffoundsperm AT nataliathomas spermfineneedleaspirationfnamappingafterfailedmicrodissectiontesticularspermextractionteselocationandpatternsoffoundsperm AT imokcha spermfineneedleaspirationfnamappingafterfailedmicrodissectiontesticularspermextractionteselocationandpatternsoffoundsperm AT kedarcheprasad spermfineneedleaspirationfnamappingafterfailedmicrodissectiontesticularspermextractionteselocationandpatternsoffoundsperm AT jonathanwaramsay spermfineneedleaspirationfnamappingafterfailedmicrodissectiontesticularspermextractionteselocationandpatternsoffoundsperm AT pauljturek spermfineneedleaspirationfnamappingafterfailedmicrodissectiontesticularspermextractionteselocationandpatternsoffoundsperm |