Excitation-Dependent Fluorescence Helps to Indicate Fungal Contamination of Aquatic Environments and to Differentiate Filamentous Fungi
Fungal contamination of aquatic environments can lead to an adverse impact on the environment and human health. (1) The search for fast, inexpensive and appropriate methods for detection of fungi is very moving rapidly due to their significant impact on ecosystem functions and human health. (2) We f...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Photonics |
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| Online Access: | https://www.mdpi.com/2304-6732/9/10/692 |
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| author | Elena Fedoseeva Svetlana Patsaeva Devard Stom Vera Terekhova |
| author_facet | Elena Fedoseeva Svetlana Patsaeva Devard Stom Vera Terekhova |
| author_sort | Elena Fedoseeva |
| collection | DOAJ |
| description | Fungal contamination of aquatic environments can lead to an adverse impact on the environment and human health. (1) The search for fast, inexpensive and appropriate methods for detection of fungi is very moving rapidly due to their significant impact on ecosystem functions and human health. (2) We focused on examination of fluorescence proxies able to distinguish chromophoric matter occurring in different fungi. Spectroscopic studies were performed on five strains of filamentous fungi: <i>Trichoderma harzianum, Fusarium solani, Alternaria alternata, Cladosporium cladosporioides</i> and <i>Aspergillus terreus</i>. (3) The results showed that most of the fungal autofluorescence was emitted by amino acids, melanin-like compounds, NAD(P)H and flavins. The spectra of five fungal species cultivated as planktonic or surface-associated forms turned out to be different. Protein fluorescence can be used to detect general microbial contamination. Presence of excitation wavelength dependent mode and the “blue shift” of fluorescence (emission bands 400–500 nm) can be suggested as specific feature of fluorescence of fungal melanin-containing samples. (4) The determination based on fluorescence spectra obtained at a certain excitation/emission wavelengths pair and at whole excitation-emission matrices (EEMs) coupled to principal component analysis (PCA) algorithms as a tool of improving detection capabilities can be suggested to enable fast and inexpensive monitoring of fungal contamination of aquatic environments. |
| first_indexed | 2024-03-09T19:33:22Z |
| format | Article |
| id | doaj.art-28d7e74c77e64480811235f76e137641 |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-03-09T19:33:22Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-28d7e74c77e64480811235f76e1376412023-11-24T02:00:56ZengMDPI AGPhotonics2304-67322022-09-0191069210.3390/photonics9100692Excitation-Dependent Fluorescence Helps to Indicate Fungal Contamination of Aquatic Environments and to Differentiate Filamentous FungiElena Fedoseeva0Svetlana Patsaeva1Devard Stom2Vera Terekhova3Laboratory of Ecological Functions of Soil, Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, 119071 Moscow, RussiaFaculty of Physics, Lomonosov Moscow State University, 119991 Moscow, RussiaFaculty of Biology and Soil, Irkutsk State University, 664003 Irkutsk, RussiaLaboratory of Ecological Functions of Soil, Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, 119071 Moscow, RussiaFungal contamination of aquatic environments can lead to an adverse impact on the environment and human health. (1) The search for fast, inexpensive and appropriate methods for detection of fungi is very moving rapidly due to their significant impact on ecosystem functions and human health. (2) We focused on examination of fluorescence proxies able to distinguish chromophoric matter occurring in different fungi. Spectroscopic studies were performed on five strains of filamentous fungi: <i>Trichoderma harzianum, Fusarium solani, Alternaria alternata, Cladosporium cladosporioides</i> and <i>Aspergillus terreus</i>. (3) The results showed that most of the fungal autofluorescence was emitted by amino acids, melanin-like compounds, NAD(P)H and flavins. The spectra of five fungal species cultivated as planktonic or surface-associated forms turned out to be different. Protein fluorescence can be used to detect general microbial contamination. Presence of excitation wavelength dependent mode and the “blue shift” of fluorescence (emission bands 400–500 nm) can be suggested as specific feature of fluorescence of fungal melanin-containing samples. (4) The determination based on fluorescence spectra obtained at a certain excitation/emission wavelengths pair and at whole excitation-emission matrices (EEMs) coupled to principal component analysis (PCA) algorithms as a tool of improving detection capabilities can be suggested to enable fast and inexpensive monitoring of fungal contamination of aquatic environments.https://www.mdpi.com/2304-6732/9/10/692fungal contaminationexcitation-dependent fluorescenceexcitation/emission matrix |
| spellingShingle | Elena Fedoseeva Svetlana Patsaeva Devard Stom Vera Terekhova Excitation-Dependent Fluorescence Helps to Indicate Fungal Contamination of Aquatic Environments and to Differentiate Filamentous Fungi Photonics fungal contamination excitation-dependent fluorescence excitation/emission matrix |
| title | Excitation-Dependent Fluorescence Helps to Indicate Fungal Contamination of Aquatic Environments and to Differentiate Filamentous Fungi |
| title_full | Excitation-Dependent Fluorescence Helps to Indicate Fungal Contamination of Aquatic Environments and to Differentiate Filamentous Fungi |
| title_fullStr | Excitation-Dependent Fluorescence Helps to Indicate Fungal Contamination of Aquatic Environments and to Differentiate Filamentous Fungi |
| title_full_unstemmed | Excitation-Dependent Fluorescence Helps to Indicate Fungal Contamination of Aquatic Environments and to Differentiate Filamentous Fungi |
| title_short | Excitation-Dependent Fluorescence Helps to Indicate Fungal Contamination of Aquatic Environments and to Differentiate Filamentous Fungi |
| title_sort | excitation dependent fluorescence helps to indicate fungal contamination of aquatic environments and to differentiate filamentous fungi |
| topic | fungal contamination excitation-dependent fluorescence excitation/emission matrix |
| url | https://www.mdpi.com/2304-6732/9/10/692 |
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