Comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among Vibrionaceae
Abstract Objectives The eps locus in Vibrio diabolicus is involved in the production of the biotechnologically valuable HE800 EPS. In this study, the distribution and diversity of similar eps gene clusters across Vibrionaceae and its variability in relation to phylogenetic relationship were investig...
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-018-3214-z |
| _version_ | 1818790315091296256 |
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| author | Lou Lebellenger Véronique Verrez-Bagnis Delphine Passerini Christine Delbarre-Ladrat |
| author_facet | Lou Lebellenger Véronique Verrez-Bagnis Delphine Passerini Christine Delbarre-Ladrat |
| author_sort | Lou Lebellenger |
| collection | DOAJ |
| description | Abstract Objectives The eps locus in Vibrio diabolicus is involved in the production of the biotechnologically valuable HE800 EPS. In this study, the distribution and diversity of similar eps gene clusters across Vibrionaceae and its variability in relation to phylogenetic relationship were investigated. The aim was to provide a better knowledge of the eps gene cluster importance and to facilitate discovery of new EPS with potent interesting bioactivities. Results Seventy percent of the 103 genome sequences examined display such an eps locus with a high level of synteny. However, genetic divergence was found inside some monophyletic clades or even between some strains of the same species. It includes gene insertions, truncations, and deletions. Comparative analysis also reveals some variations in glycosyltransferase and export systems genes. Phylogenetic analysis of the Vibrionaceae eps gene clusters within Vibrionaceae suggests a vertical transfer by speciation but also pinpoints rearrangement events independent of the speciation. |
| first_indexed | 2024-12-18T14:53:30Z |
| format | Article |
| id | doaj.art-28d838e21bb840d49231e24bef27ea5f |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-18T14:53:30Z |
| publishDate | 2018-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-28d838e21bb840d49231e24bef27ea5f2022-12-21T21:04:06ZengBMCBMC Research Notes1756-05002018-02-011111710.1186/s13104-018-3214-zComparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among VibrionaceaeLou Lebellenger0Véronique Verrez-Bagnis1Delphine Passerini2Christine Delbarre-Ladrat3Ifremer, Atlantique Center, Biotechnology and Marine Resources Unit, Marine Ecosystems and Marine Molecules for the Biotechnologies LaboratoryIfremer, Atlantique Center, Biotechnology and Marine Resources Unit, Marine Ecosystems and Marine Molecules for the Biotechnologies LaboratoryIfremer, Atlantique Center, Biotechnology and Marine Resources Unit, Marine Ecosystems and Marine Molecules for the Biotechnologies LaboratoryIfremer, Atlantique Center, Biotechnology and Marine Resources Unit, Marine Ecosystems and Marine Molecules for the Biotechnologies LaboratoryAbstract Objectives The eps locus in Vibrio diabolicus is involved in the production of the biotechnologically valuable HE800 EPS. In this study, the distribution and diversity of similar eps gene clusters across Vibrionaceae and its variability in relation to phylogenetic relationship were investigated. The aim was to provide a better knowledge of the eps gene cluster importance and to facilitate discovery of new EPS with potent interesting bioactivities. Results Seventy percent of the 103 genome sequences examined display such an eps locus with a high level of synteny. However, genetic divergence was found inside some monophyletic clades or even between some strains of the same species. It includes gene insertions, truncations, and deletions. Comparative analysis also reveals some variations in glycosyltransferase and export systems genes. Phylogenetic analysis of the Vibrionaceae eps gene clusters within Vibrionaceae suggests a vertical transfer by speciation but also pinpoints rearrangement events independent of the speciation.http://link.springer.com/article/10.1186/s13104-018-3214-zVibrionaceaeExopolysaccharideGenetic biodiversityBiosynthesisVibrio diabolicusHE800 EPS |
| spellingShingle | Lou Lebellenger Véronique Verrez-Bagnis Delphine Passerini Christine Delbarre-Ladrat Comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among Vibrionaceae BMC Research Notes Vibrionaceae Exopolysaccharide Genetic biodiversity Biosynthesis Vibrio diabolicus HE800 EPS |
| title | Comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among Vibrionaceae |
| title_full | Comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among Vibrionaceae |
| title_fullStr | Comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among Vibrionaceae |
| title_full_unstemmed | Comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among Vibrionaceae |
| title_short | Comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among Vibrionaceae |
| title_sort | comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among vibrionaceae |
| topic | Vibrionaceae Exopolysaccharide Genetic biodiversity Biosynthesis Vibrio diabolicus HE800 EPS |
| url | http://link.springer.com/article/10.1186/s13104-018-3214-z |
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