Antimicrobial compounds were isolated from the secondary metabolites of Gordonia, a resident of intestinal tract of Periplaneta americana
Abstract Gordonia sp. are members of the actinomycete family, their contribution to the environment improvement and environmental protection by their biological degradation ability, but there are few studies on the antimicrobial activity of their secondary metabolites. Our team isolated and purified...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-07-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-021-01272-y |
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| author | Yan Ma Minhua Xu Hancong Liu Tiantian Yu Ping Guo Wenbin Liu Xiaobao Jin |
| author_facet | Yan Ma Minhua Xu Hancong Liu Tiantian Yu Ping Guo Wenbin Liu Xiaobao Jin |
| author_sort | Yan Ma |
| collection | DOAJ |
| description | Abstract Gordonia sp. are members of the actinomycete family, their contribution to the environment improvement and environmental protection by their biological degradation ability, but there are few studies on the antimicrobial activity of their secondary metabolites. Our team isolated and purified an actinomycete WA 4-31 from the intestinal tract of Periplaneta americana, firstly identified the strain WA 4-31 by the morphological characteristics and the phylogenetic analyses, and found it was completely homologous to the strain of Gordonia terrae from the Indian desert. Meanwhile, actinomycin D (1), actinomycin X2 (2), mojavensin A (3) and cyclic (leucine-leucne) dipeptide (4) were obtained from the EtOAc extract from the broth of WA 4-31. Compounds 1–4 showed anti-fungus activities against Candida albicans, Aspergillus niger, A. fumigatus and Trichophyton rubrum, also anti-MRSA and inhibited Escherichia coli in different degree. Interestingly, we found when 3 was mixed with 4 with ratio of 1:1, the activity of the mixture on anti-Candida albicans was better than the single. Besides, compounds 1–3 had varying degrees of antiproliferative activities on CNE-2 and HepG-2 cell lines. These indicated that Gordonia rare actinomycete from the intestinal tract of Periplaneta americana possessed a potential as a source of active secondary metabolites. |
| first_indexed | 2024-12-22T08:19:28Z |
| format | Article |
| id | doaj.art-28d87533eff7428db57217e65d305ad3 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-22T08:19:28Z |
| publishDate | 2021-07-01 |
| publisher | SpringerOpen |
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| series | AMB Express |
| spelling | doaj.art-28d87533eff7428db57217e65d305ad32022-12-21T18:32:47ZengSpringerOpenAMB Express2191-08552021-07-0111111110.1186/s13568-021-01272-yAntimicrobial compounds were isolated from the secondary metabolites of Gordonia, a resident of intestinal tract of Periplaneta americanaYan Ma0Minhua Xu1Hancong Liu2Tiantian Yu3Ping Guo4Wenbin Liu5Xiaobao Jin6School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical UniversitySchool of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical UniversitySchool of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical UniversitySchool of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical UniversitySchool of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical UniversitySchool of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical UniversitySchool of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical UniversityAbstract Gordonia sp. are members of the actinomycete family, their contribution to the environment improvement and environmental protection by their biological degradation ability, but there are few studies on the antimicrobial activity of their secondary metabolites. Our team isolated and purified an actinomycete WA 4-31 from the intestinal tract of Periplaneta americana, firstly identified the strain WA 4-31 by the morphological characteristics and the phylogenetic analyses, and found it was completely homologous to the strain of Gordonia terrae from the Indian desert. Meanwhile, actinomycin D (1), actinomycin X2 (2), mojavensin A (3) and cyclic (leucine-leucne) dipeptide (4) were obtained from the EtOAc extract from the broth of WA 4-31. Compounds 1–4 showed anti-fungus activities against Candida albicans, Aspergillus niger, A. fumigatus and Trichophyton rubrum, also anti-MRSA and inhibited Escherichia coli in different degree. Interestingly, we found when 3 was mixed with 4 with ratio of 1:1, the activity of the mixture on anti-Candida albicans was better than the single. Besides, compounds 1–3 had varying degrees of antiproliferative activities on CNE-2 and HepG-2 cell lines. These indicated that Gordonia rare actinomycete from the intestinal tract of Periplaneta americana possessed a potential as a source of active secondary metabolites.https://doi.org/10.1186/s13568-021-01272-yGordoniaSecondary metabolitesAntimicrobial activityAntiproliferative activityPeriplaneta americana |
| spellingShingle | Yan Ma Minhua Xu Hancong Liu Tiantian Yu Ping Guo Wenbin Liu Xiaobao Jin Antimicrobial compounds were isolated from the secondary metabolites of Gordonia, a resident of intestinal tract of Periplaneta americana AMB Express Gordonia Secondary metabolites Antimicrobial activity Antiproliferative activity Periplaneta americana |
| title | Antimicrobial compounds were isolated from the secondary metabolites of Gordonia, a resident of intestinal tract of Periplaneta americana |
| title_full | Antimicrobial compounds were isolated from the secondary metabolites of Gordonia, a resident of intestinal tract of Periplaneta americana |
| title_fullStr | Antimicrobial compounds were isolated from the secondary metabolites of Gordonia, a resident of intestinal tract of Periplaneta americana |
| title_full_unstemmed | Antimicrobial compounds were isolated from the secondary metabolites of Gordonia, a resident of intestinal tract of Periplaneta americana |
| title_short | Antimicrobial compounds were isolated from the secondary metabolites of Gordonia, a resident of intestinal tract of Periplaneta americana |
| title_sort | antimicrobial compounds were isolated from the secondary metabolites of gordonia a resident of intestinal tract of periplaneta americana |
| topic | Gordonia Secondary metabolites Antimicrobial activity Antiproliferative activity Periplaneta americana |
| url | https://doi.org/10.1186/s13568-021-01272-y |
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