Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484
Background: The aim of this study was to test the effect of TNF484 on cell proliferation, migration, and invasion of hepatocellular carcinoma (HCC) cells. Materials and Methods: Various doses (0, 1, 10, 50, and 100 nM) of TNF484 were applied to the HepG2 and Bel7402 cells, and cell proliferation was...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Journal of Research in Medical Sciences |
| Subjects: | |
| Online Access: | http://www.jmsjournal.net/article.asp?issn=1735-1995;year=2019;volume=24;issue=1;spage=26;epage=26;aulast=Xia |
| _version_ | 1811310095491923968 |
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| author | Changhong Xia Dongsheng Zhang Yanmei Li Jie Chen Haibo Zhou Long Nie Yanyan Sun Siyan Guo Jianbiao Cao Fangzheng Zhou Junlai Li |
| author_facet | Changhong Xia Dongsheng Zhang Yanmei Li Jie Chen Haibo Zhou Long Nie Yanyan Sun Siyan Guo Jianbiao Cao Fangzheng Zhou Junlai Li |
| author_sort | Changhong Xia |
| collection | DOAJ |
| description | Background: The aim of this study was to test the effect of TNF484 on cell proliferation, migration, and invasion of hepatocellular carcinoma (HCC) cells. Materials and Methods: Various doses (0, 1, 10, 50, and 100 nM) of TNF484 were applied to the HepG2 and Bel7402 cells, and cell proliferation was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay after 72 h. Cell migration rate was measured using the xCELLigence system, and the cell invasion ability was examined by the three-dimensional spheroid BME cell invasion assay. The expression level of ADAM17 was also measured with RT-PCR. Results: With the treatment of TNF484, the cell proliferation of HepG2 and Bel7402 cells was inhibited in a dose-dependent manner. Moreover, under TNF484 treatment, the cell migration rate as well as cell invasion ability of the HepG2 and Bel7402 cells were suppressed. Conclusion: TNF484 could inhibit the cell proliferation, migration, and invasion of some HCC cell lines, making it a potential therapeutic option for liver cancer treatment. |
| first_indexed | 2024-04-13T09:52:40Z |
| format | Article |
| id | doaj.art-28dd0ee5ae03462c9e0773b41ccfe22d |
| institution | Directory Open Access Journal |
| issn | 1735-1995 1735-7136 |
| language | English |
| last_indexed | 2024-04-13T09:52:40Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Research in Medical Sciences |
| spelling | doaj.art-28dd0ee5ae03462c9e0773b41ccfe22d2022-12-22T02:51:32ZengWolters Kluwer Medknow PublicationsJournal of Research in Medical Sciences1735-19951735-71362019-01-01241262610.4103/jrms.JRMS_129_17Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484Changhong XiaDongsheng ZhangYanmei LiJie ChenHaibo ZhouLong NieYanyan SunSiyan GuoJianbiao CaoFangzheng ZhouJunlai LiBackground: The aim of this study was to test the effect of TNF484 on cell proliferation, migration, and invasion of hepatocellular carcinoma (HCC) cells. Materials and Methods: Various doses (0, 1, 10, 50, and 100 nM) of TNF484 were applied to the HepG2 and Bel7402 cells, and cell proliferation was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay after 72 h. Cell migration rate was measured using the xCELLigence system, and the cell invasion ability was examined by the three-dimensional spheroid BME cell invasion assay. The expression level of ADAM17 was also measured with RT-PCR. Results: With the treatment of TNF484, the cell proliferation of HepG2 and Bel7402 cells was inhibited in a dose-dependent manner. Moreover, under TNF484 treatment, the cell migration rate as well as cell invasion ability of the HepG2 and Bel7402 cells were suppressed. Conclusion: TNF484 could inhibit the cell proliferation, migration, and invasion of some HCC cell lines, making it a potential therapeutic option for liver cancer treatment.http://www.jmsjournal.net/article.asp?issn=1735-1995;year=2019;volume=24;issue=1;spage=26;epage=26;aulast=Xiaadam17hepatocellular carcinomatnf484 |
| spellingShingle | Changhong Xia Dongsheng Zhang Yanmei Li Jie Chen Haibo Zhou Long Nie Yanyan Sun Siyan Guo Jianbiao Cao Fangzheng Zhou Junlai Li Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484 Journal of Research in Medical Sciences adam17 hepatocellular carcinoma tnf484 |
| title | Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484 |
| title_full | Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484 |
| title_fullStr | Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484 |
| title_full_unstemmed | Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484 |
| title_short | Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484 |
| title_sort | inhibition of hepatocellular carcinoma cell proliferation migration and invasion by a disintegrin and metalloproteinase 17 inhibitor tnf484 |
| topic | adam17 hepatocellular carcinoma tnf484 |
| url | http://www.jmsjournal.net/article.asp?issn=1735-1995;year=2019;volume=24;issue=1;spage=26;epage=26;aulast=Xia |
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