Effects of Co-Inoculating <i>Saccharomyces</i> spp. with <i>Bradyrhizobium japonicum</i> on Atmospheric Nitrogen Fixation in Soybeans (<i>Glycine max</i> (L.))
Crop production encounters challenges due to the dearth of nitrogen (N) and phosphorus (P), while excessive chemical fertilizer use causes environmental hazards. The use of N-fixing microbes and P-solubilizing microbes (PSMs) can be a sustainable strategy to overcome these problems. Here, we conduct...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/3/681 |
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| author | Obey Kudakwashe Zveushe Victor Resco de Dios Hengxing Zhang Fang Zeng Siqin Liu Songrong Shen Qianlin Kang Yazhen Zhang Miao Huang Ahmed Sarfaraz Matina Prajapati Lei Zhou Wei Zhang Ying Han Faqin Dong |
| author_facet | Obey Kudakwashe Zveushe Victor Resco de Dios Hengxing Zhang Fang Zeng Siqin Liu Songrong Shen Qianlin Kang Yazhen Zhang Miao Huang Ahmed Sarfaraz Matina Prajapati Lei Zhou Wei Zhang Ying Han Faqin Dong |
| author_sort | Obey Kudakwashe Zveushe |
| collection | DOAJ |
| description | Crop production encounters challenges due to the dearth of nitrogen (N) and phosphorus (P), while excessive chemical fertilizer use causes environmental hazards. The use of N-fixing microbes and P-solubilizing microbes (PSMs) can be a sustainable strategy to overcome these problems. Here, we conducted a greenhouse pot experiment following a completely randomized blocked design to elucidate the influence of co-inoculating N-fixing bacteria (<i>Bradyrhizobium japonicum</i>) and PSMs (<i>Saccharomyces cerevisiae</i> and <i>Saccharomyces exiguus</i>) on atmospheric N<sub>2</sub>-fixation, growth, and yield. The results indicate a significant influence of interaction on Indole-3-acetic acid production, P solubilization, seedling germination, and growth. It was also found that atmospheric N<sub>2</sub>-fixation, nodule number per plant, nodule dry weight, straw, and root dry weight per plant at different growth stages were significantly increased under dual inoculation treatments relative to single inoculation or no inoculation treatment. Increased seed yield and N and P accumulation were also noticed under co-inoculation treatments. Soil available N was highest under sole bacterial inoculation and lowest under the control treatment, while soil available P was highest under co-inoculation treatments and lowest under the control treatment. We demonstrated that the co-inoculation of N-fixing bacteria and PSMs enhances P bioavailability and atmospheric N<sub>2</sub>-fixation in soybeans leading to improved soil fertility, raising crop yields, and promoting sustainable agriculture. |
| first_indexed | 2024-03-11T09:29:10Z |
| format | Article |
| id | doaj.art-28e2a519295f48e0a313c5017d16cf4d |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T09:29:10Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-28e2a519295f48e0a313c5017d16cf4d2023-11-16T17:45:53ZengMDPI AGPlants2223-77472023-02-0112368110.3390/plants12030681Effects of Co-Inoculating <i>Saccharomyces</i> spp. with <i>Bradyrhizobium japonicum</i> on Atmospheric Nitrogen Fixation in Soybeans (<i>Glycine max</i> (L.))Obey Kudakwashe Zveushe0Victor Resco de Dios1Hengxing Zhang2Fang Zeng3Siqin Liu4Songrong Shen5Qianlin Kang6Yazhen Zhang7Miao Huang8Ahmed Sarfaraz9Matina Prajapati10Lei Zhou11Wei Zhang12Ying Han13Faqin Dong14School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaFundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Southwest University of Science and Technology, Mianyang 621010, ChinaCenter of Analysis and Testing, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Environment and Resource, Southwest University of Science and Technology, Mianyang 621010, ChinaCrop production encounters challenges due to the dearth of nitrogen (N) and phosphorus (P), while excessive chemical fertilizer use causes environmental hazards. The use of N-fixing microbes and P-solubilizing microbes (PSMs) can be a sustainable strategy to overcome these problems. Here, we conducted a greenhouse pot experiment following a completely randomized blocked design to elucidate the influence of co-inoculating N-fixing bacteria (<i>Bradyrhizobium japonicum</i>) and PSMs (<i>Saccharomyces cerevisiae</i> and <i>Saccharomyces exiguus</i>) on atmospheric N<sub>2</sub>-fixation, growth, and yield. The results indicate a significant influence of interaction on Indole-3-acetic acid production, P solubilization, seedling germination, and growth. It was also found that atmospheric N<sub>2</sub>-fixation, nodule number per plant, nodule dry weight, straw, and root dry weight per plant at different growth stages were significantly increased under dual inoculation treatments relative to single inoculation or no inoculation treatment. Increased seed yield and N and P accumulation were also noticed under co-inoculation treatments. Soil available N was highest under sole bacterial inoculation and lowest under the control treatment, while soil available P was highest under co-inoculation treatments and lowest under the control treatment. We demonstrated that the co-inoculation of N-fixing bacteria and PSMs enhances P bioavailability and atmospheric N<sub>2</sub>-fixation in soybeans leading to improved soil fertility, raising crop yields, and promoting sustainable agriculture.https://www.mdpi.com/2223-7747/12/3/681co-inoculationnitrogen fixation ratesoybean<i>Saccharomyces cerevisiae</i><i>Saccharomyces exiguus</i><i>Bradyrhizobium japonicum</i> |
| spellingShingle | Obey Kudakwashe Zveushe Victor Resco de Dios Hengxing Zhang Fang Zeng Siqin Liu Songrong Shen Qianlin Kang Yazhen Zhang Miao Huang Ahmed Sarfaraz Matina Prajapati Lei Zhou Wei Zhang Ying Han Faqin Dong Effects of Co-Inoculating <i>Saccharomyces</i> spp. with <i>Bradyrhizobium japonicum</i> on Atmospheric Nitrogen Fixation in Soybeans (<i>Glycine max</i> (L.)) Plants co-inoculation nitrogen fixation rate soybean <i>Saccharomyces cerevisiae</i> <i>Saccharomyces exiguus</i> <i>Bradyrhizobium japonicum</i> |
| title | Effects of Co-Inoculating <i>Saccharomyces</i> spp. with <i>Bradyrhizobium japonicum</i> on Atmospheric Nitrogen Fixation in Soybeans (<i>Glycine max</i> (L.)) |
| title_full | Effects of Co-Inoculating <i>Saccharomyces</i> spp. with <i>Bradyrhizobium japonicum</i> on Atmospheric Nitrogen Fixation in Soybeans (<i>Glycine max</i> (L.)) |
| title_fullStr | Effects of Co-Inoculating <i>Saccharomyces</i> spp. with <i>Bradyrhizobium japonicum</i> on Atmospheric Nitrogen Fixation in Soybeans (<i>Glycine max</i> (L.)) |
| title_full_unstemmed | Effects of Co-Inoculating <i>Saccharomyces</i> spp. with <i>Bradyrhizobium japonicum</i> on Atmospheric Nitrogen Fixation in Soybeans (<i>Glycine max</i> (L.)) |
| title_short | Effects of Co-Inoculating <i>Saccharomyces</i> spp. with <i>Bradyrhizobium japonicum</i> on Atmospheric Nitrogen Fixation in Soybeans (<i>Glycine max</i> (L.)) |
| title_sort | effects of co inoculating i saccharomyces i spp with i bradyrhizobium japonicum i on atmospheric nitrogen fixation in soybeans i glycine max i l |
| topic | co-inoculation nitrogen fixation rate soybean <i>Saccharomyces cerevisiae</i> <i>Saccharomyces exiguus</i> <i>Bradyrhizobium japonicum</i> |
| url | https://www.mdpi.com/2223-7747/12/3/681 |
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