<it>Medicago truncatula </it>and <it>Glomus intraradices </it>gene expression in cortical cells harboring arbuscules in the arbuscular mycorrhizal symbiosis
<p>Abstract</p> <p>Background</p> <p>Most vascular flowering plants have the capacity to form symbiotic associations with arbuscular mycorrhizal (AM) fungi. The symbiosis develops in the roots where AM fungi colonize the root cortex and form arbuscules within the cortic...
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-01-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/9/10 |
| _version_ | 1818135452175040512 |
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| author | Tang Yuhong Torres-Jerez Ivone Deewatthanawong Prasit Javot Hélène Gomez S Karen Blancaflor Elison B Udvardi Michael K Harrison Maria J |
| author_facet | Tang Yuhong Torres-Jerez Ivone Deewatthanawong Prasit Javot Hélène Gomez S Karen Blancaflor Elison B Udvardi Michael K Harrison Maria J |
| author_sort | Tang Yuhong |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Most vascular flowering plants have the capacity to form symbiotic associations with arbuscular mycorrhizal (AM) fungi. The symbiosis develops in the roots where AM fungi colonize the root cortex and form arbuscules within the cortical cells. Arbuscules are enveloped in a novel plant membrane and their establishment requires the coordinated cellular activities of both symbiotic partners. The arbuscule-cortical cell interface is the primary functional interface of the symbiosis and is of central importance in nutrient exchange. To determine the molecular events the underlie arbuscule development and function, it is first necessary to identify genes that may play a role in this process. Toward this goal we used the Affymetrix GeneChip<sup>® </sup>Medicago Genome Array to document the <it>M. truncatula </it>transcript profiles associated with AM symbiosis, and then developed laser microdissection (LM) of <it>M. truncatula </it>root cortical cells to enable analyses of gene expression in individual cell types by RT-PCR.</p> <p>Results</p> <p>This approach led to the identification of novel <it>M. truncatula </it>and <it>G. intraradices </it>genes expressed in colonized cortical cells and in arbuscules. Within the arbuscule, expression of genes associated with the urea cycle, amino acid biosynthesis and cellular autophagy was detected. Analysis of gene expression in the colonized cortical cell revealed up-regulation of a lysine motif (LysM)-receptor like kinase, members of the GRAS transcription factor family and a symbiosis-specific ammonium transporter that is a likely candidate for mediating ammonium transport in the AM symbiosis.</p> <p>Conclusion</p> <p>Transcript profiling using the Affymetrix GeneChip<sup>® </sup>Medicago Genome Array provided new insights into gene expression in <it>M. truncatula </it>roots during AM symbiosis and revealed the existence of several <it>G. intraradices </it>genes on the <it>M. truncatula </it>GeneChip<sup>®</sup>. A laser microdissection protocol that incorporates low-melting temperature Steedman's wax, was developed to enable laser microdissection of <it>M. truncatula </it>root cortical cells. LM coupled with RT-PCR provided spatial gene expression information for both symbionts and expanded current information available for gene expression in cortical cells containing arbuscules.</p> |
| first_indexed | 2024-12-11T09:24:44Z |
| format | Article |
| id | doaj.art-28e4cff0eb1c42bdb74bdeb76af907ed |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-11T09:24:44Z |
| publishDate | 2009-01-01 |
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| spelling | doaj.art-28e4cff0eb1c42bdb74bdeb76af907ed2022-12-22T01:13:11ZengBMCBMC Plant Biology1471-22292009-01-01911010.1186/1471-2229-9-10<it>Medicago truncatula </it>and <it>Glomus intraradices </it>gene expression in cortical cells harboring arbuscules in the arbuscular mycorrhizal symbiosisTang YuhongTorres-Jerez IvoneDeewatthanawong PrasitJavot HélèneGomez S KarenBlancaflor Elison BUdvardi Michael KHarrison Maria J<p>Abstract</p> <p>Background</p> <p>Most vascular flowering plants have the capacity to form symbiotic associations with arbuscular mycorrhizal (AM) fungi. The symbiosis develops in the roots where AM fungi colonize the root cortex and form arbuscules within the cortical cells. Arbuscules are enveloped in a novel plant membrane and their establishment requires the coordinated cellular activities of both symbiotic partners. The arbuscule-cortical cell interface is the primary functional interface of the symbiosis and is of central importance in nutrient exchange. To determine the molecular events the underlie arbuscule development and function, it is first necessary to identify genes that may play a role in this process. Toward this goal we used the Affymetrix GeneChip<sup>® </sup>Medicago Genome Array to document the <it>M. truncatula </it>transcript profiles associated with AM symbiosis, and then developed laser microdissection (LM) of <it>M. truncatula </it>root cortical cells to enable analyses of gene expression in individual cell types by RT-PCR.</p> <p>Results</p> <p>This approach led to the identification of novel <it>M. truncatula </it>and <it>G. intraradices </it>genes expressed in colonized cortical cells and in arbuscules. Within the arbuscule, expression of genes associated with the urea cycle, amino acid biosynthesis and cellular autophagy was detected. Analysis of gene expression in the colonized cortical cell revealed up-regulation of a lysine motif (LysM)-receptor like kinase, members of the GRAS transcription factor family and a symbiosis-specific ammonium transporter that is a likely candidate for mediating ammonium transport in the AM symbiosis.</p> <p>Conclusion</p> <p>Transcript profiling using the Affymetrix GeneChip<sup>® </sup>Medicago Genome Array provided new insights into gene expression in <it>M. truncatula </it>roots during AM symbiosis and revealed the existence of several <it>G. intraradices </it>genes on the <it>M. truncatula </it>GeneChip<sup>®</sup>. A laser microdissection protocol that incorporates low-melting temperature Steedman's wax, was developed to enable laser microdissection of <it>M. truncatula </it>root cortical cells. LM coupled with RT-PCR provided spatial gene expression information for both symbionts and expanded current information available for gene expression in cortical cells containing arbuscules.</p>http://www.biomedcentral.com/1471-2229/9/10 |
| spellingShingle | Tang Yuhong Torres-Jerez Ivone Deewatthanawong Prasit Javot Hélène Gomez S Karen Blancaflor Elison B Udvardi Michael K Harrison Maria J <it>Medicago truncatula </it>and <it>Glomus intraradices </it>gene expression in cortical cells harboring arbuscules in the arbuscular mycorrhizal symbiosis BMC Plant Biology |
| title | <it>Medicago truncatula </it>and <it>Glomus intraradices </it>gene expression in cortical cells harboring arbuscules in the arbuscular mycorrhizal symbiosis |
| title_full | <it>Medicago truncatula </it>and <it>Glomus intraradices </it>gene expression in cortical cells harboring arbuscules in the arbuscular mycorrhizal symbiosis |
| title_fullStr | <it>Medicago truncatula </it>and <it>Glomus intraradices </it>gene expression in cortical cells harboring arbuscules in the arbuscular mycorrhizal symbiosis |
| title_full_unstemmed | <it>Medicago truncatula </it>and <it>Glomus intraradices </it>gene expression in cortical cells harboring arbuscules in the arbuscular mycorrhizal symbiosis |
| title_short | <it>Medicago truncatula </it>and <it>Glomus intraradices </it>gene expression in cortical cells harboring arbuscules in the arbuscular mycorrhizal symbiosis |
| title_sort | it medicago truncatula it and it glomus intraradices it gene expression in cortical cells harboring arbuscules in the arbuscular mycorrhizal symbiosis |
| url | http://www.biomedcentral.com/1471-2229/9/10 |
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