Expression and clinical significance of Cathepsin K and MMPs in invasive non-functioning pituitary adenomas
BackgroundCathepsin K (CTSK) is a protease that degrades type I collagen and extracellular matrix, thereby contributing to bone resorption and tumor invasion. Some pituitary adenomas (PAs) could invade the sphenoid sinus (SS) and cavernous sinus (CS).PurposeThis retrospective cohort study aimed to s...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.901647/full |
| _version_ | 1828151680891355136 |
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| author | Hongyan Liu Hongyan Liu Saichun Zhang Ting Wu Ting Wu Zhaohui Lv Zhaohui Lv Jianming Ba Weijun Gu Weijun Gu Yiming Mu Yiming Mu |
| author_facet | Hongyan Liu Hongyan Liu Saichun Zhang Ting Wu Ting Wu Zhaohui Lv Zhaohui Lv Jianming Ba Weijun Gu Weijun Gu Yiming Mu Yiming Mu |
| author_sort | Hongyan Liu |
| collection | DOAJ |
| description | BackgroundCathepsin K (CTSK) is a protease that degrades type I collagen and extracellular matrix, thereby contributing to bone resorption and tumor invasion. Some pituitary adenomas (PAs) could invade the sphenoid sinus (SS) and cavernous sinus (CS).PurposeThis retrospective cohort study aimed to study the expression of tumoral biomarkers (CTSK, MMP9, MMP2, TIMP2, and PTTG1) and evaluate their clinical significance in non-functioning pituitary adenomas (NFPAs) with different invasion patterns.MethodsWe assessed the expression levels of candidate invasion-specific protein biomarkers CTSK, MMP9, MMP2, TIMP2, and PTTG1 by immunohistochemical staining in paraffin-embedded NFPA tumor tissues. Variations in staining intensity were analyzed in cases with SS and CS invasion and non-invasive NFPAs.ResultsWe found that the levels of CTSK were higher in PA cases with SS invasion than that in PA cases with CS invasion (95.57 ± 31.57 vs. 65.29 ± 29.64, P < 0.001), and the expression of MMP9 and MMP2 was higher in CS-invasive cases than that in SS-invasive cases (145.02 ± 49.25 vs. 111.80 ± 51.37, P = 0.002, and 138.67 ± 52.06 vs. 108.30 ± 41.70, P = 0.002). Multiple Cox regression demonstrated that higher CTSK expression (P=0.011), subtotal resection (P<0.001), invasion (P=0.037), and larger tumor diameter (P=0.001) were independent risk factors for recurrence. A positive correlation was observed between CTSK expression and tumor size (r=0.671, p<0.001). There was no significant difference in TIMP2 and PTTG1 levels between CS-and SS-invasive cases (97.42± 39.80 vs. 102.10± 43.22, P = 0.58 and 13.89 ± 4.59 vs. 12.56 ± 3.96, P = 0.14).ConclusionOur data indicated that CTSK has the potential as a marker for SS invasion of PAs, whereas MMP9 and MMP2 may be markers for CS invasion. And CTSK may play an important role in tumor relapse. |
| first_indexed | 2024-04-11T22:01:05Z |
| format | Article |
| id | doaj.art-28ea35b21c0c40338233646c8cde8634 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-11T22:01:05Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-28ea35b21c0c40338233646c8cde86342022-12-22T04:00:55ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-08-011210.3389/fonc.2022.901647901647Expression and clinical significance of Cathepsin K and MMPs in invasive non-functioning pituitary adenomasHongyan Liu0Hongyan Liu1Saichun Zhang2Ting Wu3Ting Wu4Zhaohui Lv5Zhaohui Lv6Jianming Ba7Weijun Gu8Weijun Gu9Yiming Mu10Yiming Mu11The Chinese PLA Medical School, Beijing, ChinaDepartment of Endocrinology, The First Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Endocrinology, The First Medical Center of Chinese PLA General Hospital, Beijing, ChinaThe Chinese PLA Medical School, Beijing, ChinaDepartment of Endocrinology, The First Medical Center of Chinese PLA General Hospital, Beijing, ChinaThe Chinese PLA Medical School, Beijing, ChinaDepartment of Endocrinology, The First Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Endocrinology, The First Medical Center of Chinese PLA General Hospital, Beijing, ChinaThe Chinese PLA Medical School, Beijing, ChinaDepartment of Endocrinology, The First Medical Center of Chinese PLA General Hospital, Beijing, ChinaThe Chinese PLA Medical School, Beijing, ChinaDepartment of Endocrinology, The First Medical Center of Chinese PLA General Hospital, Beijing, ChinaBackgroundCathepsin K (CTSK) is a protease that degrades type I collagen and extracellular matrix, thereby contributing to bone resorption and tumor invasion. Some pituitary adenomas (PAs) could invade the sphenoid sinus (SS) and cavernous sinus (CS).PurposeThis retrospective cohort study aimed to study the expression of tumoral biomarkers (CTSK, MMP9, MMP2, TIMP2, and PTTG1) and evaluate their clinical significance in non-functioning pituitary adenomas (NFPAs) with different invasion patterns.MethodsWe assessed the expression levels of candidate invasion-specific protein biomarkers CTSK, MMP9, MMP2, TIMP2, and PTTG1 by immunohistochemical staining in paraffin-embedded NFPA tumor tissues. Variations in staining intensity were analyzed in cases with SS and CS invasion and non-invasive NFPAs.ResultsWe found that the levels of CTSK were higher in PA cases with SS invasion than that in PA cases with CS invasion (95.57 ± 31.57 vs. 65.29 ± 29.64, P < 0.001), and the expression of MMP9 and MMP2 was higher in CS-invasive cases than that in SS-invasive cases (145.02 ± 49.25 vs. 111.80 ± 51.37, P = 0.002, and 138.67 ± 52.06 vs. 108.30 ± 41.70, P = 0.002). Multiple Cox regression demonstrated that higher CTSK expression (P=0.011), subtotal resection (P<0.001), invasion (P=0.037), and larger tumor diameter (P=0.001) were independent risk factors for recurrence. A positive correlation was observed between CTSK expression and tumor size (r=0.671, p<0.001). There was no significant difference in TIMP2 and PTTG1 levels between CS-and SS-invasive cases (97.42± 39.80 vs. 102.10± 43.22, P = 0.58 and 13.89 ± 4.59 vs. 12.56 ± 3.96, P = 0.14).ConclusionOur data indicated that CTSK has the potential as a marker for SS invasion of PAs, whereas MMP9 and MMP2 may be markers for CS invasion. And CTSK may play an important role in tumor relapse.https://www.frontiersin.org/articles/10.3389/fonc.2022.901647/fullCathepsin Ksphenoid sinuspituitary adenomasinvasion |
| spellingShingle | Hongyan Liu Hongyan Liu Saichun Zhang Ting Wu Ting Wu Zhaohui Lv Zhaohui Lv Jianming Ba Weijun Gu Weijun Gu Yiming Mu Yiming Mu Expression and clinical significance of Cathepsin K and MMPs in invasive non-functioning pituitary adenomas Frontiers in Oncology Cathepsin K sphenoid sinus pituitary adenomas invasion |
| title | Expression and clinical significance of Cathepsin K and MMPs in invasive non-functioning pituitary adenomas |
| title_full | Expression and clinical significance of Cathepsin K and MMPs in invasive non-functioning pituitary adenomas |
| title_fullStr | Expression and clinical significance of Cathepsin K and MMPs in invasive non-functioning pituitary adenomas |
| title_full_unstemmed | Expression and clinical significance of Cathepsin K and MMPs in invasive non-functioning pituitary adenomas |
| title_short | Expression and clinical significance of Cathepsin K and MMPs in invasive non-functioning pituitary adenomas |
| title_sort | expression and clinical significance of cathepsin k and mmps in invasive non functioning pituitary adenomas |
| topic | Cathepsin K sphenoid sinus pituitary adenomas invasion |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.901647/full |
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