Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium
Clinically relevant members of the fungal genus, Fusarium, exhibit an extraordinary genetic diversity and cause a wide spectrum of infections in both healthy individuals and immunocompromised patients. Generally, Fusarium species are intrinsically resistant to all systemic antifungals. We investigat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-01-01
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| Series: | Journal of Fungi |
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| Online Access: | http://www.mdpi.com/2309-608X/4/1/16 |
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| author | Hafize Sav Haleh Rafati Yasemin Öz Burcu Dalyan-Cilo Beyza Ener Faezeh Mohammadi Macit Ilkit Anne D. van Diepeningen Seyedmojtaba Seyedmousavi |
| author_facet | Hafize Sav Haleh Rafati Yasemin Öz Burcu Dalyan-Cilo Beyza Ener Faezeh Mohammadi Macit Ilkit Anne D. van Diepeningen Seyedmojtaba Seyedmousavi |
| author_sort | Hafize Sav |
| collection | DOAJ |
| description | Clinically relevant members of the fungal genus, Fusarium, exhibit an extraordinary genetic diversity and cause a wide spectrum of infections in both healthy individuals and immunocompromised patients. Generally, Fusarium species are intrinsically resistant to all systemic antifungals. We investigated whether the presence or absence of the ability to produce biofilms across and within Fusarium species complexes is linked to higher resistance against antifungals. A collection of 41 Fusarium strains, obtained from 38 patients with superficial and systemic infections, and three infected crops, were tested, including 25 species within the Fusarium fujikuroi species complex, 14 from the Fusarium solani species complex (FSSC), one Fusarium dimerum species complex, and one Fusarium oxysporum species complex isolate. Of all isolates tested, only seven strains from two species of FSSC, five F. petroliphilum and two F. keratoplasticum strains, recovered from blood, nail scrapings, and nasal biopsy samples, could produce biofilms under the tested conditions. In the liquid culture tested, sessile biofilm-forming Fusarium strains exhibited elevated minimum inhibitory concentrations (MICs) for amphotericin B, voriconazole, and posaconazole, compared to their planktonic counterparts, indicating that the ability to form biofilm may significantly increase resistance. Collectively, this suggests that once a surface adherent biofilm has been established, therapies designed to kill planktonic cells of Fusarium are ineffective. |
| first_indexed | 2024-04-12T21:58:02Z |
| format | Article |
| id | doaj.art-28ef5749fa5d4116b3c10abedc200360 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-04-12T21:58:02Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-28ef5749fa5d4116b3c10abedc2003602022-12-22T03:15:13ZengMDPI AGJournal of Fungi2309-608X2018-01-01411610.3390/jof4010016jof4010016Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus FusariumHafize Sav0Haleh Rafati1Yasemin Öz2Burcu Dalyan-Cilo3Beyza Ener4Faezeh Mohammadi5Macit Ilkit6Anne D. van Diepeningen7Seyedmojtaba Seyedmousavi8Department of Mycology, Kayseri Education and Research Hospital, Kayseri 38010, TurkeyDepartment of Microbiology and Immunology, Center of Excellence for Infection Biology and Antimicrobial Pharmacology, Tehran 1969753491, IranDivision of Mycology, Department of Microbiology, Faculty of Medicine, University of Osmangazi, Eskişehir 26040, TurkeyDivision of Mycology, Department of Medical Microbiology, University of Health Sciences Bursa High Specialization Training and Research Hospital, Bursa 16320, TurkeyDepartment of Microbiology, Faculty of Medicine, Uludağ University, Bursa 16059, TurkeyDepartment of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin 34156-13911, IranDivision of Mycology, Department of Microbiology, Faculty of Medicine, University of Çukurova, Adana 01330, TurkeyWesterdijk Fungal Biodiversity Institute, 3584 CT Utrecht, The NetherlandsDepartment of Microbiology and Immunology, Center of Excellence for Infection Biology and Antimicrobial Pharmacology, Tehran 1969753491, IranClinically relevant members of the fungal genus, Fusarium, exhibit an extraordinary genetic diversity and cause a wide spectrum of infections in both healthy individuals and immunocompromised patients. Generally, Fusarium species are intrinsically resistant to all systemic antifungals. We investigated whether the presence or absence of the ability to produce biofilms across and within Fusarium species complexes is linked to higher resistance against antifungals. A collection of 41 Fusarium strains, obtained from 38 patients with superficial and systemic infections, and three infected crops, were tested, including 25 species within the Fusarium fujikuroi species complex, 14 from the Fusarium solani species complex (FSSC), one Fusarium dimerum species complex, and one Fusarium oxysporum species complex isolate. Of all isolates tested, only seven strains from two species of FSSC, five F. petroliphilum and two F. keratoplasticum strains, recovered from blood, nail scrapings, and nasal biopsy samples, could produce biofilms under the tested conditions. In the liquid culture tested, sessile biofilm-forming Fusarium strains exhibited elevated minimum inhibitory concentrations (MICs) for amphotericin B, voriconazole, and posaconazole, compared to their planktonic counterparts, indicating that the ability to form biofilm may significantly increase resistance. Collectively, this suggests that once a surface adherent biofilm has been established, therapies designed to kill planktonic cells of Fusarium are ineffective.http://www.mdpi.com/2309-608X/4/1/16biofilmsFusarium solani species complexFusarium petroliphilumFusarium keratoplasticumantifungal resistance |
| spellingShingle | Hafize Sav Haleh Rafati Yasemin Öz Burcu Dalyan-Cilo Beyza Ener Faezeh Mohammadi Macit Ilkit Anne D. van Diepeningen Seyedmojtaba Seyedmousavi Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium Journal of Fungi biofilms Fusarium solani species complex Fusarium petroliphilum Fusarium keratoplasticum antifungal resistance |
| title | Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium |
| title_full | Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium |
| title_fullStr | Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium |
| title_full_unstemmed | Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium |
| title_short | Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium |
| title_sort | biofilm formation and resistance to fungicides in clinically relevant members of the fungal genus fusarium |
| topic | biofilms Fusarium solani species complex Fusarium petroliphilum Fusarium keratoplasticum antifungal resistance |
| url | http://www.mdpi.com/2309-608X/4/1/16 |
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