Identification of the Interaction between Minichromosome Maintenance Proteins and the Core Protein of Hepatitis B Virus
Chronic HBV infection is a major cause of cirrhosis and hepatocellular carcinoma. Finding host factors involved in the viral life cycle and elucidating their mechanisms is essential for developing innovative strategies for treating HBV. The HBV core protein has pleiotropic roles in HBV replication;...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/1/50 |
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| author | Kaili Du Eriko Ohsaki Masami Wada Keiji Ueda |
| author_facet | Kaili Du Eriko Ohsaki Masami Wada Keiji Ueda |
| author_sort | Kaili Du |
| collection | DOAJ |
| description | Chronic HBV infection is a major cause of cirrhosis and hepatocellular carcinoma. Finding host factors involved in the viral life cycle and elucidating their mechanisms is essential for developing innovative strategies for treating HBV. The HBV core protein has pleiotropic roles in HBV replication; thus, finding the interactions between the core protein and host factors is important in clarifying the mechanism of viral infection and proliferation. Recent studies have revealed that core proteins are involved in cccDNA formation, transcriptional regulation, and RNA metabolism, in addition to their primary functions of capsid formation and pgRNA packaging. Here, we report the interaction of the core protein with MCMs, which have an essential role in host DNA replication. The knockdown of MCM2 led to increased viral replication during infection, suggesting that MCM2 serves as a restriction factor for HBV proliferation. This study opens the possibility of elucidating the relationship between core proteins and host factors and their function in viral proliferation. |
| first_indexed | 2024-03-09T13:07:23Z |
| format | Article |
| id | doaj.art-28f07668bddf4279bdd5e7fc426c7416 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-09T13:07:23Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-28f07668bddf4279bdd5e7fc426c74162023-11-30T21:45:41ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-01-0145175276410.3390/cimb45010050Identification of the Interaction between Minichromosome Maintenance Proteins and the Core Protein of Hepatitis B VirusKaili Du0Eriko Ohsaki1Masami Wada2Keiji Ueda3Division of Virology, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita 565-0871, JapanDivision of Virology, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita 565-0871, JapanDivision of Virology, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita 565-0871, JapanDivision of Virology, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita 565-0871, JapanChronic HBV infection is a major cause of cirrhosis and hepatocellular carcinoma. Finding host factors involved in the viral life cycle and elucidating their mechanisms is essential for developing innovative strategies for treating HBV. The HBV core protein has pleiotropic roles in HBV replication; thus, finding the interactions between the core protein and host factors is important in clarifying the mechanism of viral infection and proliferation. Recent studies have revealed that core proteins are involved in cccDNA formation, transcriptional regulation, and RNA metabolism, in addition to their primary functions of capsid formation and pgRNA packaging. Here, we report the interaction of the core protein with MCMs, which have an essential role in host DNA replication. The knockdown of MCM2 led to increased viral replication during infection, suggesting that MCM2 serves as a restriction factor for HBV proliferation. This study opens the possibility of elucidating the relationship between core proteins and host factors and their function in viral proliferation.https://www.mdpi.com/1467-3045/45/1/50hepatitis B virusHBVcore proteinminichromosome maintenance proteinsMCMsDNA replication |
| spellingShingle | Kaili Du Eriko Ohsaki Masami Wada Keiji Ueda Identification of the Interaction between Minichromosome Maintenance Proteins and the Core Protein of Hepatitis B Virus Current Issues in Molecular Biology hepatitis B virus HBV core protein minichromosome maintenance proteins MCMs DNA replication |
| title | Identification of the Interaction between Minichromosome Maintenance Proteins and the Core Protein of Hepatitis B Virus |
| title_full | Identification of the Interaction between Minichromosome Maintenance Proteins and the Core Protein of Hepatitis B Virus |
| title_fullStr | Identification of the Interaction between Minichromosome Maintenance Proteins and the Core Protein of Hepatitis B Virus |
| title_full_unstemmed | Identification of the Interaction between Minichromosome Maintenance Proteins and the Core Protein of Hepatitis B Virus |
| title_short | Identification of the Interaction between Minichromosome Maintenance Proteins and the Core Protein of Hepatitis B Virus |
| title_sort | identification of the interaction between minichromosome maintenance proteins and the core protein of hepatitis b virus |
| topic | hepatitis B virus HBV core protein minichromosome maintenance proteins MCMs DNA replication |
| url | https://www.mdpi.com/1467-3045/45/1/50 |
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