A SNP-Based Molecular Barcode for Characterization of Common Wheat.
Wheat is grown as a staple crop worldwide. It is important to develop an effective genotyping tool for this cereal grain both to identify germplasm diversity and to protect the rights of breeders. Single-nucleotide polymorphism (SNP) genotyping provides a means for developing a practical, rapid, ine...
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Format: | Article |
Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4795793?pdf=render |
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author | LiFeng Gao JiZeng Jia XiuYing Kong |
author_facet | LiFeng Gao JiZeng Jia XiuYing Kong |
author_sort | LiFeng Gao |
collection | DOAJ |
description | Wheat is grown as a staple crop worldwide. It is important to develop an effective genotyping tool for this cereal grain both to identify germplasm diversity and to protect the rights of breeders. Single-nucleotide polymorphism (SNP) genotyping provides a means for developing a practical, rapid, inexpensive and high-throughput assay. Here, we investigated SNPs as robust markers of genetic variation for typing wheat cultivars. We identified SNPs from an array of 9000 across a collection of 429 well-known wheat cultivars grown in China, of which 43 SNP markers with high minor allele frequency and variations discriminated the selected wheat varieties and their wild ancestors. This SNP-based barcode will allow for the rapid and precise identification of wheat germplasm resources and newly released varieties and will further assist in the wheat breeding program. |
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format | Article |
id | doaj.art-28f09ac6b13a47929bae9518a6892359 |
institution | Directory Open Access Journal |
issn | 1932-6203 |
language | English |
last_indexed | 2024-12-10T14:38:52Z |
publishDate | 2016-01-01 |
publisher | Public Library of Science (PLoS) |
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series | PLoS ONE |
spelling | doaj.art-28f09ac6b13a47929bae9518a68923592022-12-22T01:44:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015094710.1371/journal.pone.0150947A SNP-Based Molecular Barcode for Characterization of Common Wheat.LiFeng GaoJiZeng JiaXiuYing KongWheat is grown as a staple crop worldwide. It is important to develop an effective genotyping tool for this cereal grain both to identify germplasm diversity and to protect the rights of breeders. Single-nucleotide polymorphism (SNP) genotyping provides a means for developing a practical, rapid, inexpensive and high-throughput assay. Here, we investigated SNPs as robust markers of genetic variation for typing wheat cultivars. We identified SNPs from an array of 9000 across a collection of 429 well-known wheat cultivars grown in China, of which 43 SNP markers with high minor allele frequency and variations discriminated the selected wheat varieties and their wild ancestors. This SNP-based barcode will allow for the rapid and precise identification of wheat germplasm resources and newly released varieties and will further assist in the wheat breeding program.http://europepmc.org/articles/PMC4795793?pdf=render |
spellingShingle | LiFeng Gao JiZeng Jia XiuYing Kong A SNP-Based Molecular Barcode for Characterization of Common Wheat. PLoS ONE |
title | A SNP-Based Molecular Barcode for Characterization of Common Wheat. |
title_full | A SNP-Based Molecular Barcode for Characterization of Common Wheat. |
title_fullStr | A SNP-Based Molecular Barcode for Characterization of Common Wheat. |
title_full_unstemmed | A SNP-Based Molecular Barcode for Characterization of Common Wheat. |
title_short | A SNP-Based Molecular Barcode for Characterization of Common Wheat. |
title_sort | snp based molecular barcode for characterization of common wheat |
url | http://europepmc.org/articles/PMC4795793?pdf=render |
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