A SNP-Based Molecular Barcode for Characterization of Common Wheat.

Wheat is grown as a staple crop worldwide. It is important to develop an effective genotyping tool for this cereal grain both to identify germplasm diversity and to protect the rights of breeders. Single-nucleotide polymorphism (SNP) genotyping provides a means for developing a practical, rapid, ine...

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Main Authors: LiFeng Gao, JiZeng Jia, XiuYing Kong
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4795793?pdf=render
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author LiFeng Gao
JiZeng Jia
XiuYing Kong
author_facet LiFeng Gao
JiZeng Jia
XiuYing Kong
author_sort LiFeng Gao
collection DOAJ
description Wheat is grown as a staple crop worldwide. It is important to develop an effective genotyping tool for this cereal grain both to identify germplasm diversity and to protect the rights of breeders. Single-nucleotide polymorphism (SNP) genotyping provides a means for developing a practical, rapid, inexpensive and high-throughput assay. Here, we investigated SNPs as robust markers of genetic variation for typing wheat cultivars. We identified SNPs from an array of 9000 across a collection of 429 well-known wheat cultivars grown in China, of which 43 SNP markers with high minor allele frequency and variations discriminated the selected wheat varieties and their wild ancestors. This SNP-based barcode will allow for the rapid and precise identification of wheat germplasm resources and newly released varieties and will further assist in the wheat breeding program.
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spelling doaj.art-28f09ac6b13a47929bae9518a68923592022-12-22T01:44:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015094710.1371/journal.pone.0150947A SNP-Based Molecular Barcode for Characterization of Common Wheat.LiFeng GaoJiZeng JiaXiuYing KongWheat is grown as a staple crop worldwide. It is important to develop an effective genotyping tool for this cereal grain both to identify germplasm diversity and to protect the rights of breeders. Single-nucleotide polymorphism (SNP) genotyping provides a means for developing a practical, rapid, inexpensive and high-throughput assay. Here, we investigated SNPs as robust markers of genetic variation for typing wheat cultivars. We identified SNPs from an array of 9000 across a collection of 429 well-known wheat cultivars grown in China, of which 43 SNP markers with high minor allele frequency and variations discriminated the selected wheat varieties and their wild ancestors. This SNP-based barcode will allow for the rapid and precise identification of wheat germplasm resources and newly released varieties and will further assist in the wheat breeding program.http://europepmc.org/articles/PMC4795793?pdf=render
spellingShingle LiFeng Gao
JiZeng Jia
XiuYing Kong
A SNP-Based Molecular Barcode for Characterization of Common Wheat.
PLoS ONE
title A SNP-Based Molecular Barcode for Characterization of Common Wheat.
title_full A SNP-Based Molecular Barcode for Characterization of Common Wheat.
title_fullStr A SNP-Based Molecular Barcode for Characterization of Common Wheat.
title_full_unstemmed A SNP-Based Molecular Barcode for Characterization of Common Wheat.
title_short A SNP-Based Molecular Barcode for Characterization of Common Wheat.
title_sort snp based molecular barcode for characterization of common wheat
url http://europepmc.org/articles/PMC4795793?pdf=render
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