Rapid antibiotic susceptibility testing and species identification for mixed samples
Rapid antibiotic susceptibility testing (AST) is needed. Here the authors report a method for phenotypic AST at the single cell level, using a microfluidic chip that allows for subsequent genotyping with in situ FISH; they apply this to a mixed sample of 7 species and 4 antibiotics.
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-33659-1 |
| _version_ | 1811249851070939136 |
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| author | Vinodh Kandavalli Praneeth Karempudi Jimmy Larsson Johan Elf |
| author_facet | Vinodh Kandavalli Praneeth Karempudi Jimmy Larsson Johan Elf |
| author_sort | Vinodh Kandavalli |
| collection | DOAJ |
| description | Rapid antibiotic susceptibility testing (AST) is needed. Here the authors report a method for phenotypic AST at the single cell level, using a microfluidic chip that allows for subsequent genotyping with in situ FISH; they apply this to a mixed sample of 7 species and 4 antibiotics. |
| first_indexed | 2024-04-12T15:54:52Z |
| format | Article |
| id | doaj.art-28f5f838d7d04d78a148d3f67e4d8c05 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-12T15:54:52Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-28f5f838d7d04d78a148d3f67e4d8c052022-12-22T03:26:24ZengNature PortfolioNature Communications2041-17232022-10-011311810.1038/s41467-022-33659-1Rapid antibiotic susceptibility testing and species identification for mixed samplesVinodh Kandavalli0Praneeth Karempudi1Jimmy Larsson2Johan Elf3Dept. Cell and Molecular Biology, Uppsala UniversityDept. Cell and Molecular Biology, Uppsala UniversityDept. Cell and Molecular Biology, Uppsala UniversityDept. Cell and Molecular Biology, Uppsala UniversityRapid antibiotic susceptibility testing (AST) is needed. Here the authors report a method for phenotypic AST at the single cell level, using a microfluidic chip that allows for subsequent genotyping with in situ FISH; they apply this to a mixed sample of 7 species and 4 antibiotics.https://doi.org/10.1038/s41467-022-33659-1 |
| spellingShingle | Vinodh Kandavalli Praneeth Karempudi Jimmy Larsson Johan Elf Rapid antibiotic susceptibility testing and species identification for mixed samples Nature Communications |
| title | Rapid antibiotic susceptibility testing and species identification for mixed samples |
| title_full | Rapid antibiotic susceptibility testing and species identification for mixed samples |
| title_fullStr | Rapid antibiotic susceptibility testing and species identification for mixed samples |
| title_full_unstemmed | Rapid antibiotic susceptibility testing and species identification for mixed samples |
| title_short | Rapid antibiotic susceptibility testing and species identification for mixed samples |
| title_sort | rapid antibiotic susceptibility testing and species identification for mixed samples |
| url | https://doi.org/10.1038/s41467-022-33659-1 |
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