Anti-arthritic Effects of Ephedra sinica STAPF Herb-Acupuncture: Inhibition of Lipopolysaccharide-Induced Inflammation and Adjuvant-Induced Polyarthritis
Abstract.: Anti-inflammatory and anti-arthritic effects of water distillates of Ephedra sinica STAPF (ES), in herb-acupuncture, on the inflammatory responses of arthritis was investigated using phorbol 12-myristate 13-acetate (PMA)/lipopolysaccharide (LPS)-induced human macro-phage and adjuvant-indu...
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2006-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319345372 |
| _version_ | 1811322898766364672 |
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| author | Mi-Jung Yeom Han-Chang Lee Gun-Ho Kim Hye-Jung Lee Insop Shim Seung-Kyu Oh Sung-Keel Kang Dae-Hyun Hahm |
| author_facet | Mi-Jung Yeom Han-Chang Lee Gun-Ho Kim Hye-Jung Lee Insop Shim Seung-Kyu Oh Sung-Keel Kang Dae-Hyun Hahm |
| author_sort | Mi-Jung Yeom |
| collection | DOAJ |
| description | Abstract.: Anti-inflammatory and anti-arthritic effects of water distillates of Ephedra sinica STAPF (ES), in herb-acupuncture, on the inflammatory responses of arthritis was investigated using phorbol 12-myristate 13-acetate (PMA)/lipopolysaccharide (LPS)-induced human macro-phage and adjuvant-induced arthritic rat. The luciferase reporter vectors driven by the tumor necrosis factor (TNF)-α and cyclooxygenase-2 promoters were transiently transfected into U937 cells, which were then differentiated and stimulated by PMA and LPS, respectively, to develop an in vitro anti-inflammation assay system. The luciferase activities, observed in the activated U937 cells, were significantly inhibited by ES herb-acupuncture, compared to those of PD98509 and berberine. To evaluate ES herb-acupuncture as a novel anti-arthritic therapy, a polyarthritic rat model was developed using heat-killed Mycobacterium tuberculosis, and 50 µl of ES distillate was subcutaneously injected into the ST36 acupoint on each knee joint. While the articular indexes of arthritic rats were evidently decreased by ES herb-acupuncture, their body weights did not regain their initial levels. This may be due to the accelerating effects of ES on weight-loss and fat consumption. The mRNA expressions of TNF-α and interleukin (IL)-6 genes, which were closely stimulated in the arthritic rat joints, were found to be restored to the normal levels through the ES treatment. In the case of IL-1β, the recovery was not significant but substantial. The anti-arthritic effect of ES herb-acupuncture was not found in the ES-treated/non-acupoint group. In conclusion, the ES herb-acupuncture into the ST36 acupoint was found to be effective in alleviating the inflammatory response and thus arthritic symptoms in adjuvant-induced arthritic rats. Keywords:: Ephedra sinica STAPF, herb-acupuncture, adjuvant-induced rat arthritis, inflammation, cytokine |
| first_indexed | 2024-04-13T13:44:23Z |
| format | Article |
| id | doaj.art-28f92fc5fa88499e869e8abed23000b4 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-04-13T13:44:23Z |
| publishDate | 2006-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-28f92fc5fa88499e869e8abed23000b42022-12-22T02:44:32ZengElsevierJournal of Pharmacological Sciences1347-86132006-01-0110014150Anti-arthritic Effects of Ephedra sinica STAPF Herb-Acupuncture: Inhibition of Lipopolysaccharide-Induced Inflammation and Adjuvant-Induced PolyarthritisMi-Jung Yeom0Han-Chang Lee1Gun-Ho Kim2Hye-Jung Lee3Insop Shim4Seung-Kyu Oh5Sung-Keel Kang6Dae-Hyun Hahm7Laboratory of Acupuncture & Meridian, Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University, Kihung-up, Yongin-si, Kyungki-do 449-701, KoreaLaboratory of Acupuncture & Meridian, Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University, Kihung-up, Yongin-si, Kyungki-do 449-701, KoreaLaboratory of Acupuncture & Meridian, Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University, Kihung-up, Yongin-si, Kyungki-do 449-701, KoreaLaboratory of Acupuncture & Meridian, Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University, Kihung-up, Yongin-si, Kyungki-do 449-701, KoreaDepartment of Integrative Medicine, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-701, KoreaLaboratory of Acupuncture & Meridian, Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University, Kihung-up, Yongin-si, Kyungki-do 449-701, KoreaDepartment of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University, Seoul 130-701, KoreaLaboratory of Acupuncture & Meridian, Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University, Kihung-up, Yongin-si, Kyungki-do 449-701, Korea; Corresponding author. Present address for correspondence: Neuroscience Research Institute, Gachon Medical School, 1198 Kuwoldong, Incheon 405-760, Korea. dhahm@paran.comAbstract.: Anti-inflammatory and anti-arthritic effects of water distillates of Ephedra sinica STAPF (ES), in herb-acupuncture, on the inflammatory responses of arthritis was investigated using phorbol 12-myristate 13-acetate (PMA)/lipopolysaccharide (LPS)-induced human macro-phage and adjuvant-induced arthritic rat. The luciferase reporter vectors driven by the tumor necrosis factor (TNF)-α and cyclooxygenase-2 promoters were transiently transfected into U937 cells, which were then differentiated and stimulated by PMA and LPS, respectively, to develop an in vitro anti-inflammation assay system. The luciferase activities, observed in the activated U937 cells, were significantly inhibited by ES herb-acupuncture, compared to those of PD98509 and berberine. To evaluate ES herb-acupuncture as a novel anti-arthritic therapy, a polyarthritic rat model was developed using heat-killed Mycobacterium tuberculosis, and 50 µl of ES distillate was subcutaneously injected into the ST36 acupoint on each knee joint. While the articular indexes of arthritic rats were evidently decreased by ES herb-acupuncture, their body weights did not regain their initial levels. This may be due to the accelerating effects of ES on weight-loss and fat consumption. The mRNA expressions of TNF-α and interleukin (IL)-6 genes, which were closely stimulated in the arthritic rat joints, were found to be restored to the normal levels through the ES treatment. In the case of IL-1β, the recovery was not significant but substantial. The anti-arthritic effect of ES herb-acupuncture was not found in the ES-treated/non-acupoint group. In conclusion, the ES herb-acupuncture into the ST36 acupoint was found to be effective in alleviating the inflammatory response and thus arthritic symptoms in adjuvant-induced arthritic rats. Keywords:: Ephedra sinica STAPF, herb-acupuncture, adjuvant-induced rat arthritis, inflammation, cytokinehttp://www.sciencedirect.com/science/article/pii/S1347861319345372 |
| spellingShingle | Mi-Jung Yeom Han-Chang Lee Gun-Ho Kim Hye-Jung Lee Insop Shim Seung-Kyu Oh Sung-Keel Kang Dae-Hyun Hahm Anti-arthritic Effects of Ephedra sinica STAPF Herb-Acupuncture: Inhibition of Lipopolysaccharide-Induced Inflammation and Adjuvant-Induced Polyarthritis Journal of Pharmacological Sciences |
| title | Anti-arthritic Effects of Ephedra sinica STAPF Herb-Acupuncture: Inhibition of Lipopolysaccharide-Induced Inflammation and Adjuvant-Induced Polyarthritis |
| title_full | Anti-arthritic Effects of Ephedra sinica STAPF Herb-Acupuncture: Inhibition of Lipopolysaccharide-Induced Inflammation and Adjuvant-Induced Polyarthritis |
| title_fullStr | Anti-arthritic Effects of Ephedra sinica STAPF Herb-Acupuncture: Inhibition of Lipopolysaccharide-Induced Inflammation and Adjuvant-Induced Polyarthritis |
| title_full_unstemmed | Anti-arthritic Effects of Ephedra sinica STAPF Herb-Acupuncture: Inhibition of Lipopolysaccharide-Induced Inflammation and Adjuvant-Induced Polyarthritis |
| title_short | Anti-arthritic Effects of Ephedra sinica STAPF Herb-Acupuncture: Inhibition of Lipopolysaccharide-Induced Inflammation and Adjuvant-Induced Polyarthritis |
| title_sort | anti arthritic effects of ephedra sinica stapf herb acupuncture inhibition of lipopolysaccharide induced inflammation and adjuvant induced polyarthritis |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319345372 |
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