Integrative transcriptomic analysis deciphering the role of rice bHLH transcription factor Os04g0301500 in mediating responses to biotic and abiotic stresses
Understanding the signaling pathways activated in response to these combined stresses and their crosstalk is crucial to breeding crop varieties with dual or multiple tolerances. However, most studies to date have predominantly focused on individual stress factors, leaving a significant gap in unders...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1266242/full |
| _version_ | 1797671788273991680 |
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| author | Qiuping Zhang Qiuping Zhang Rong Teng Rong Teng Ziyi Yuan Ziyi Yuan Song Sheng Song Sheng Yunhua Xiao Yunhua Xiao Huabing Deng Huabing Deng Wenbang Tang Wenbang Tang Feng Wang Feng Wang |
| author_facet | Qiuping Zhang Qiuping Zhang Rong Teng Rong Teng Ziyi Yuan Ziyi Yuan Song Sheng Song Sheng Yunhua Xiao Yunhua Xiao Huabing Deng Huabing Deng Wenbang Tang Wenbang Tang Feng Wang Feng Wang |
| author_sort | Qiuping Zhang |
| collection | DOAJ |
| description | Understanding the signaling pathways activated in response to these combined stresses and their crosstalk is crucial to breeding crop varieties with dual or multiple tolerances. However, most studies to date have predominantly focused on individual stress factors, leaving a significant gap in understanding plant responses to combined biotic and abiotic stresses. The bHLH family plays a multifaceted regulatory role in plant response to both abiotic and biotic stresses. In order to comprehensively identify and analyze the bHLH gene family in rice, we identified putative OsbHLHs by multi-step homolog search, and phylogenic analysis, molecular weights, isoelectric points, conserved domain screening were processed using MEGAX version 10.2.6. Following, integrative transcriptome analysis using 6 RNA-seq data including Xoo infection, heat, and cold stress was processed. The results showed that 106 OsbHLHs were identified and clustered into 17 clades. Os04g0301500 and Os04g0489600 are potential negative regulators of Xoo resistance in rice. In addition, Os04g0301500 was involved in non-freezing temperatures (around 4°C) but not to 10°C cold stresses, suggesting a complex interplay with temperature signaling pathways. The study concludes that Os04g0301500 may play a crucial role in integrating biotic and abiotic stress responses in rice, potentially serving as a key regulator of plant resilience under changing environmental conditions, which could be important for further multiple stresses enhancement and molecular breeding through genetic engineering in rice. |
| first_indexed | 2024-03-11T21:20:38Z |
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| id | doaj.art-28fa1840872445e5b9c16305e27d68a5 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-11T21:20:38Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-28fa1840872445e5b9c16305e27d68a52023-09-28T06:08:00ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-09-011410.3389/fpls.2023.12662421266242Integrative transcriptomic analysis deciphering the role of rice bHLH transcription factor Os04g0301500 in mediating responses to biotic and abiotic stressesQiuping Zhang0Qiuping Zhang1Rong Teng2Rong Teng3Ziyi Yuan4Ziyi Yuan5Song Sheng6Song Sheng7Yunhua Xiao8Yunhua Xiao9Huabing Deng10Huabing Deng11Wenbang Tang12Wenbang Tang13Feng Wang14Feng Wang15College of Agronomy, Hunan Agricultural University, Changsha, ChinaYuelushan Laboratory, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaYuelushan Laboratory, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaYuelushan Laboratory, Changsha, ChinaYuelushan Laboratory, Changsha, ChinaCollege of Forest, Central South University of Forestry and Technology, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaYuelushan Laboratory, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaHunan Hybrid Rice Centre, Hunan Academy of Agricultural Science, Changsha, ChinaYuelushan Laboratory, Changsha, ChinaHunan Hybrid Rice Centre, Hunan Academy of Agricultural Science, Changsha, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha, ChinaYuelushan Laboratory, Changsha, ChinaUnderstanding the signaling pathways activated in response to these combined stresses and their crosstalk is crucial to breeding crop varieties with dual or multiple tolerances. However, most studies to date have predominantly focused on individual stress factors, leaving a significant gap in understanding plant responses to combined biotic and abiotic stresses. The bHLH family plays a multifaceted regulatory role in plant response to both abiotic and biotic stresses. In order to comprehensively identify and analyze the bHLH gene family in rice, we identified putative OsbHLHs by multi-step homolog search, and phylogenic analysis, molecular weights, isoelectric points, conserved domain screening were processed using MEGAX version 10.2.6. Following, integrative transcriptome analysis using 6 RNA-seq data including Xoo infection, heat, and cold stress was processed. The results showed that 106 OsbHLHs were identified and clustered into 17 clades. Os04g0301500 and Os04g0489600 are potential negative regulators of Xoo resistance in rice. In addition, Os04g0301500 was involved in non-freezing temperatures (around 4°C) but not to 10°C cold stresses, suggesting a complex interplay with temperature signaling pathways. The study concludes that Os04g0301500 may play a crucial role in integrating biotic and abiotic stress responses in rice, potentially serving as a key regulator of plant resilience under changing environmental conditions, which could be important for further multiple stresses enhancement and molecular breeding through genetic engineering in rice.https://www.frontiersin.org/articles/10.3389/fpls.2023.1266242/fullintegrative transcriptomic analysisricemulti-step homolog searchOsbHLHsbiotic and abiotic stress |
| spellingShingle | Qiuping Zhang Qiuping Zhang Rong Teng Rong Teng Ziyi Yuan Ziyi Yuan Song Sheng Song Sheng Yunhua Xiao Yunhua Xiao Huabing Deng Huabing Deng Wenbang Tang Wenbang Tang Feng Wang Feng Wang Integrative transcriptomic analysis deciphering the role of rice bHLH transcription factor Os04g0301500 in mediating responses to biotic and abiotic stresses Frontiers in Plant Science integrative transcriptomic analysis rice multi-step homolog search OsbHLHs biotic and abiotic stress |
| title | Integrative transcriptomic analysis deciphering the role of rice bHLH transcription factor Os04g0301500 in mediating responses to biotic and abiotic stresses |
| title_full | Integrative transcriptomic analysis deciphering the role of rice bHLH transcription factor Os04g0301500 in mediating responses to biotic and abiotic stresses |
| title_fullStr | Integrative transcriptomic analysis deciphering the role of rice bHLH transcription factor Os04g0301500 in mediating responses to biotic and abiotic stresses |
| title_full_unstemmed | Integrative transcriptomic analysis deciphering the role of rice bHLH transcription factor Os04g0301500 in mediating responses to biotic and abiotic stresses |
| title_short | Integrative transcriptomic analysis deciphering the role of rice bHLH transcription factor Os04g0301500 in mediating responses to biotic and abiotic stresses |
| title_sort | integrative transcriptomic analysis deciphering the role of rice bhlh transcription factor os04g0301500 in mediating responses to biotic and abiotic stresses |
| topic | integrative transcriptomic analysis rice multi-step homolog search OsbHLHs biotic and abiotic stress |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1266242/full |
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