Genome-Wide Identification of the Ginkgo (<i>Ginkgo biloba</i> L.) <i>LBD</i> Transcription Factor Gene and Characterization of Its Expression
Lateral organ boundaries domain (LBD) proteins are plant-specific transcription factors involved in various transcriptional regulation processes. We identified a total of 37 <i>GbLBD</i> genes in ginkgo, and based on gene structure and phylogenetic analysis, the <i>GbLBD</i>...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/10/5474 |
| _version_ | 1797499209246572544 |
|---|---|
| author | Yating Tian Xin Han Yanshu Qu Yifan Zhang Hao Rong Kai Wu Li’an Xu |
| author_facet | Yating Tian Xin Han Yanshu Qu Yifan Zhang Hao Rong Kai Wu Li’an Xu |
| author_sort | Yating Tian |
| collection | DOAJ |
| description | Lateral organ boundaries domain (LBD) proteins are plant-specific transcription factors involved in various transcriptional regulation processes. We identified a total of 37 <i>GbLBD</i> genes in ginkgo, and based on gene structure and phylogenetic analysis, the <i>GbLBD</i> gene family was classified into class I (33, with the largest number of Id genes (16)) and class II (4). The ginkgo <i>LBD</i> gene was also analyzed regarding its chromosomal distributions, gene duplications, promoters, and introns/exons. In addition, gene expression profiling and real-time quantitative PCR analysis showed that the expression of 14 <i>GbLBD</i> genes differed in six different tissues and three developmental stages. The <i>GbLBD</i> gene of class II were highly expressed relative to the class I gene in all tissues and developmental stages, while class Id gene were generally at low levels or were not expressed, especially in seed developmental stages. The expression pattern analysis of cold/drought treatment and IAA/ABA hormone treatment showed that abiotic stress treatment could significantly induce the expression of <i>GbLBD</i> gene, of which class II genes played a key role in stress treatment. Our study provides a solid foundation for further evolutionary and functional analysis of the ginkgo <i>LBD</i> gene family. |
| first_indexed | 2024-03-10T03:44:11Z |
| format | Article |
| id | doaj.art-28fd06c8b8d149f08e4b8f722ac78b07 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:44:11Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-28fd06c8b8d149f08e4b8f722ac78b072023-11-23T11:23:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310547410.3390/ijms23105474Genome-Wide Identification of the Ginkgo (<i>Ginkgo biloba</i> L.) <i>LBD</i> Transcription Factor Gene and Characterization of Its ExpressionYating Tian0Xin Han1Yanshu Qu2Yifan Zhang3Hao Rong4Kai Wu5Li’an Xu6Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, ChinaLateral organ boundaries domain (LBD) proteins are plant-specific transcription factors involved in various transcriptional regulation processes. We identified a total of 37 <i>GbLBD</i> genes in ginkgo, and based on gene structure and phylogenetic analysis, the <i>GbLBD</i> gene family was classified into class I (33, with the largest number of Id genes (16)) and class II (4). The ginkgo <i>LBD</i> gene was also analyzed regarding its chromosomal distributions, gene duplications, promoters, and introns/exons. In addition, gene expression profiling and real-time quantitative PCR analysis showed that the expression of 14 <i>GbLBD</i> genes differed in six different tissues and three developmental stages. The <i>GbLBD</i> gene of class II were highly expressed relative to the class I gene in all tissues and developmental stages, while class Id gene were generally at low levels or were not expressed, especially in seed developmental stages. The expression pattern analysis of cold/drought treatment and IAA/ABA hormone treatment showed that abiotic stress treatment could significantly induce the expression of <i>GbLBD</i> gene, of which class II genes played a key role in stress treatment. Our study provides a solid foundation for further evolutionary and functional analysis of the ginkgo <i>LBD</i> gene family.https://www.mdpi.com/1422-0067/23/10/5474<i>Ginkgo biloba</i><i>LBD</i> gene familyexpression patternstress physiology |
| spellingShingle | Yating Tian Xin Han Yanshu Qu Yifan Zhang Hao Rong Kai Wu Li’an Xu Genome-Wide Identification of the Ginkgo (<i>Ginkgo biloba</i> L.) <i>LBD</i> Transcription Factor Gene and Characterization of Its Expression International Journal of Molecular Sciences <i>Ginkgo biloba</i> <i>LBD</i> gene family expression pattern stress physiology |
| title | Genome-Wide Identification of the Ginkgo (<i>Ginkgo biloba</i> L.) <i>LBD</i> Transcription Factor Gene and Characterization of Its Expression |
| title_full | Genome-Wide Identification of the Ginkgo (<i>Ginkgo biloba</i> L.) <i>LBD</i> Transcription Factor Gene and Characterization of Its Expression |
| title_fullStr | Genome-Wide Identification of the Ginkgo (<i>Ginkgo biloba</i> L.) <i>LBD</i> Transcription Factor Gene and Characterization of Its Expression |
| title_full_unstemmed | Genome-Wide Identification of the Ginkgo (<i>Ginkgo biloba</i> L.) <i>LBD</i> Transcription Factor Gene and Characterization of Its Expression |
| title_short | Genome-Wide Identification of the Ginkgo (<i>Ginkgo biloba</i> L.) <i>LBD</i> Transcription Factor Gene and Characterization of Its Expression |
| title_sort | genome wide identification of the ginkgo i ginkgo biloba i l i lbd i transcription factor gene and characterization of its expression |
| topic | <i>Ginkgo biloba</i> <i>LBD</i> gene family expression pattern stress physiology |
| url | https://www.mdpi.com/1422-0067/23/10/5474 |
| work_keys_str_mv | AT yatingtian genomewideidentificationoftheginkgoiginkgobilobaililbditranscriptionfactorgeneandcharacterizationofitsexpression AT xinhan genomewideidentificationoftheginkgoiginkgobilobaililbditranscriptionfactorgeneandcharacterizationofitsexpression AT yanshuqu genomewideidentificationoftheginkgoiginkgobilobaililbditranscriptionfactorgeneandcharacterizationofitsexpression AT yifanzhang genomewideidentificationoftheginkgoiginkgobilobaililbditranscriptionfactorgeneandcharacterizationofitsexpression AT haorong genomewideidentificationoftheginkgoiginkgobilobaililbditranscriptionfactorgeneandcharacterizationofitsexpression AT kaiwu genomewideidentificationoftheginkgoiginkgobilobaililbditranscriptionfactorgeneandcharacterizationofitsexpression AT lianxu genomewideidentificationoftheginkgoiginkgobilobaililbditranscriptionfactorgeneandcharacterizationofitsexpression |