Computational and Experimental Investigation of the Combined Effect of Various 3D Scaffolds and Bioreactor Stimulation on Human Cells’ Feedback
Computational methods were combined with an experimental setup in order to investigate the response of human umbilical cord stem cells to 3D electrospun and printed scaffolds, when dynamically stimulated in a bioreactor. Key parameters associated to bioreactor working conditions were computationally...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Applied Biosciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2813-0464/2/2/18 |
| _version_ | 1827738820751130624 |
|---|---|
| author | Foteini K. Kozaniti Aikaterini E. Manara Vassilis Kostopoulos Panagiotis Mallis Efstathios Michalopoulos Demosthenes Polyzos Despina D. Deligianni Diana V. Portan |
| author_facet | Foteini K. Kozaniti Aikaterini E. Manara Vassilis Kostopoulos Panagiotis Mallis Efstathios Michalopoulos Demosthenes Polyzos Despina D. Deligianni Diana V. Portan |
| author_sort | Foteini K. Kozaniti |
| collection | DOAJ |
| description | Computational methods were combined with an experimental setup in order to investigate the response of human umbilical cord stem cells to 3D electrospun and printed scaffolds, when dynamically stimulated in a bioreactor. Key parameters associated to bioreactor working conditions were computationally investigated using Comsol software to use the output for the planned experimental setup. Based on the theoretical observations, the influence of the inlet velocity, cell number, and exposure time in the bioreactor were analyzed and the in vitro parameters were adjusted accordingly. MSCs were seeded in different numbers in the 3D porous scaffolds and stimulated in the bioreactor (0.5 and 2 h duration, 3 and 6 mm/s inlet velocity). Polycaprolactone 3D electrospun, and polyurethane and polylactic acid 3D-printed scaffolds were fabricated and fibronectin-coated. The computational study predicted initial events in the process of cells deposition and attachment. Total protein, osteopontin, and osteocalcin levels in cells deposited in scaffolds were investigated; SEM and confocal imaging confirmed the biomarker analysis. MSCs proliferated well in PCL. Polyurethane enabled extremely rapid proliferation followed by differentiation, while PLA induced a moderate proliferation and parallel mineralization. The scaffolds stiffness has been found as the key enabling parameter decisive for cells feedback. |
| first_indexed | 2024-03-11T02:49:26Z |
| format | Article |
| id | doaj.art-28fd503a1aa44fdbb7e1bd80abc0e9c6 |
| institution | Directory Open Access Journal |
| issn | 2813-0464 |
| language | English |
| last_indexed | 2024-03-11T02:49:26Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Biosciences |
| spelling | doaj.art-28fd503a1aa44fdbb7e1bd80abc0e9c62023-11-18T09:04:34ZengMDPI AGApplied Biosciences2813-04642023-06-012224927710.3390/applbiosci2020018Computational and Experimental Investigation of the Combined Effect of Various 3D Scaffolds and Bioreactor Stimulation on Human Cells’ FeedbackFoteini K. Kozaniti0Aikaterini E. Manara1Vassilis Kostopoulos2Panagiotis Mallis3Efstathios Michalopoulos4Demosthenes Polyzos5Despina D. Deligianni6Diana V. Portan7Laboratory of Biomechanics and Biomedical Engineering, Department of Mechanical Engineering and Aeronautics, University of Patras, 265 04 Patras, GreeceLaboratory of Biomechanics and Biomedical Engineering, Department of Mechanical Engineering and Aeronautics, University of Patras, 265 04 Patras, GreeceApplied Mechanics & Vibrations Laboratory, Department of Mechanical Engineering and Aeronautics, University of Patras, 265 04 Patras, GreeceHellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, GreeceHellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, GreeceApplied Mechanics & Vibrations Laboratory, Department of Mechanical Engineering and Aeronautics, University of Patras, 265 04 Patras, GreeceLaboratory of Biomechanics and Biomedical Engineering, Department of Mechanical Engineering and Aeronautics, University of Patras, 265 04 Patras, GreeceLaboratory of Biomechanics and Biomedical Engineering, Department of Mechanical Engineering and Aeronautics, University of Patras, 265 04 Patras, GreeceComputational methods were combined with an experimental setup in order to investigate the response of human umbilical cord stem cells to 3D electrospun and printed scaffolds, when dynamically stimulated in a bioreactor. Key parameters associated to bioreactor working conditions were computationally investigated using Comsol software to use the output for the planned experimental setup. Based on the theoretical observations, the influence of the inlet velocity, cell number, and exposure time in the bioreactor were analyzed and the in vitro parameters were adjusted accordingly. MSCs were seeded in different numbers in the 3D porous scaffolds and stimulated in the bioreactor (0.5 and 2 h duration, 3 and 6 mm/s inlet velocity). Polycaprolactone 3D electrospun, and polyurethane and polylactic acid 3D-printed scaffolds were fabricated and fibronectin-coated. The computational study predicted initial events in the process of cells deposition and attachment. Total protein, osteopontin, and osteocalcin levels in cells deposited in scaffolds were investigated; SEM and confocal imaging confirmed the biomarker analysis. MSCs proliferated well in PCL. Polyurethane enabled extremely rapid proliferation followed by differentiation, while PLA induced a moderate proliferation and parallel mineralization. The scaffolds stiffness has been found as the key enabling parameter decisive for cells feedback.https://www.mdpi.com/2813-0464/2/2/18Comsol biomedicinebioreactor3D scaffoldsstem cells in bioreactor |
| spellingShingle | Foteini K. Kozaniti Aikaterini E. Manara Vassilis Kostopoulos Panagiotis Mallis Efstathios Michalopoulos Demosthenes Polyzos Despina D. Deligianni Diana V. Portan Computational and Experimental Investigation of the Combined Effect of Various 3D Scaffolds and Bioreactor Stimulation on Human Cells’ Feedback Applied Biosciences Comsol biomedicine bioreactor 3D scaffolds stem cells in bioreactor |
| title | Computational and Experimental Investigation of the Combined Effect of Various 3D Scaffolds and Bioreactor Stimulation on Human Cells’ Feedback |
| title_full | Computational and Experimental Investigation of the Combined Effect of Various 3D Scaffolds and Bioreactor Stimulation on Human Cells’ Feedback |
| title_fullStr | Computational and Experimental Investigation of the Combined Effect of Various 3D Scaffolds and Bioreactor Stimulation on Human Cells’ Feedback |
| title_full_unstemmed | Computational and Experimental Investigation of the Combined Effect of Various 3D Scaffolds and Bioreactor Stimulation on Human Cells’ Feedback |
| title_short | Computational and Experimental Investigation of the Combined Effect of Various 3D Scaffolds and Bioreactor Stimulation on Human Cells’ Feedback |
| title_sort | computational and experimental investigation of the combined effect of various 3d scaffolds and bioreactor stimulation on human cells feedback |
| topic | Comsol biomedicine bioreactor 3D scaffolds stem cells in bioreactor |
| url | https://www.mdpi.com/2813-0464/2/2/18 |
| work_keys_str_mv | AT foteinikkozaniti computationalandexperimentalinvestigationofthecombinedeffectofvarious3dscaffoldsandbioreactorstimulationonhumancellsfeedback AT aikateriniemanara computationalandexperimentalinvestigationofthecombinedeffectofvarious3dscaffoldsandbioreactorstimulationonhumancellsfeedback AT vassiliskostopoulos computationalandexperimentalinvestigationofthecombinedeffectofvarious3dscaffoldsandbioreactorstimulationonhumancellsfeedback AT panagiotismallis computationalandexperimentalinvestigationofthecombinedeffectofvarious3dscaffoldsandbioreactorstimulationonhumancellsfeedback AT efstathiosmichalopoulos computationalandexperimentalinvestigationofthecombinedeffectofvarious3dscaffoldsandbioreactorstimulationonhumancellsfeedback AT demosthenespolyzos computationalandexperimentalinvestigationofthecombinedeffectofvarious3dscaffoldsandbioreactorstimulationonhumancellsfeedback AT despinaddeligianni computationalandexperimentalinvestigationofthecombinedeffectofvarious3dscaffoldsandbioreactorstimulationonhumancellsfeedback AT dianavportan computationalandexperimentalinvestigationofthecombinedeffectofvarious3dscaffoldsandbioreactorstimulationonhumancellsfeedback |