Novel isolate of Cladosporium subuliforme and its potential to control Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae)
Abstract Background Citrus fruits are economically and nutritionally important but have been severely affected by Huanglongbing disease (HLB), its natural spread is mainly by the Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae). Chemicals are often used to control this pest, but...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-04-01
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| Series: | Egyptian Journal of Biological Pest Control |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41938-023-00685-0 |
| _version_ | 1797849885415833600 |
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| author | Ning Wang Song Zhang Yi-Jie Li Ya-Qin Song Cui-Yun Lei Yuan-Yuan Peng Jin-Jun Wang Bing-Hai Lou Hong-Bo Jiang |
| author_facet | Ning Wang Song Zhang Yi-Jie Li Ya-Qin Song Cui-Yun Lei Yuan-Yuan Peng Jin-Jun Wang Bing-Hai Lou Hong-Bo Jiang |
| author_sort | Ning Wang |
| collection | DOAJ |
| description | Abstract Background Citrus fruits are economically and nutritionally important but have been severely affected by Huanglongbing disease (HLB), its natural spread is mainly by the Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae). Chemicals are often used to control this pest, but this is not sustainable. Meanwhile, there are few environmentally friendly bioinsecticides to control D. citri in China. Results In this study, an entomofungal pathogen wz-1 was isolated from a D. citri carcass in the field, which resulted in a cumulative mortality rate of 75.27% in adult D. citri 7 days after inoculation of the spore suspension. It was identified as Cladosporium subuliforme based on morphological analysis as well as sequence analysis of several molecular markers (Internal Transcribed Spacers, Translation Elongation Factor 1-α and Actin). Remarkably, the lethality rate of adult D. citri reached 53.13%, 48 h after treatment with the aqueous phase extracts of wz-1. Hydroxyquinoline and phytosphingosine in the extracts were identified as potentially active metabolites using LC–MS. Conclusions The entomopathogenicity and bioinsecticidal potential of C. subuliforme were previously unknown. Obtained results showed that both spores and extracts of wz-1 can effectively kill adult D. citri, providing an available fungal resource and a theoretical basis for biocontrol of the HLB insect vector D. citri. |
| first_indexed | 2024-04-09T18:51:23Z |
| format | Article |
| id | doaj.art-28fd9464e6d34936b4041be718d77b4a |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-04-09T18:51:23Z |
| publishDate | 2023-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-28fd9464e6d34936b4041be718d77b4a2023-04-09T11:26:24ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422023-04-013311910.1186/s41938-023-00685-0Novel isolate of Cladosporium subuliforme and its potential to control Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae)Ning Wang0Song Zhang1Yi-Jie Li2Ya-Qin Song3Cui-Yun Lei4Yuan-Yuan Peng5Jin-Jun Wang6Bing-Hai Lou7Hong-Bo Jiang8Guangxi Citrus Breeding and Cultivation Research Center of Engineering Technology, Guangxi Academy of Specialty CropsGuangxi Citrus Breeding and Cultivation Research Center of Engineering Technology, Guangxi Academy of Specialty CropsGuangxi Citrus Breeding and Cultivation Research Center of Engineering Technology, Guangxi Academy of Specialty CropsGuangxi Citrus Breeding and Cultivation Research Center of Engineering Technology, Guangxi Academy of Specialty CropsGuangxi Citrus Breeding and Cultivation Research Center of Engineering Technology, Guangxi Academy of Specialty CropsKey Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest UniversityKey Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest UniversityGuangxi Citrus Breeding and Cultivation Research Center of Engineering Technology, Guangxi Academy of Specialty CropsKey Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest UniversityAbstract Background Citrus fruits are economically and nutritionally important but have been severely affected by Huanglongbing disease (HLB), its natural spread is mainly by the Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae). Chemicals are often used to control this pest, but this is not sustainable. Meanwhile, there are few environmentally friendly bioinsecticides to control D. citri in China. Results In this study, an entomofungal pathogen wz-1 was isolated from a D. citri carcass in the field, which resulted in a cumulative mortality rate of 75.27% in adult D. citri 7 days after inoculation of the spore suspension. It was identified as Cladosporium subuliforme based on morphological analysis as well as sequence analysis of several molecular markers (Internal Transcribed Spacers, Translation Elongation Factor 1-α and Actin). Remarkably, the lethality rate of adult D. citri reached 53.13%, 48 h after treatment with the aqueous phase extracts of wz-1. Hydroxyquinoline and phytosphingosine in the extracts were identified as potentially active metabolites using LC–MS. Conclusions The entomopathogenicity and bioinsecticidal potential of C. subuliforme were previously unknown. Obtained results showed that both spores and extracts of wz-1 can effectively kill adult D. citri, providing an available fungal resource and a theoretical basis for biocontrol of the HLB insect vector D. citri.https://doi.org/10.1186/s41938-023-00685-0BiocontrolDiaphorina citriCladosporium subuliformeSecondary metabolitesEntomopathogenic fungus |
| spellingShingle | Ning Wang Song Zhang Yi-Jie Li Ya-Qin Song Cui-Yun Lei Yuan-Yuan Peng Jin-Jun Wang Bing-Hai Lou Hong-Bo Jiang Novel isolate of Cladosporium subuliforme and its potential to control Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae) Egyptian Journal of Biological Pest Control Biocontrol Diaphorina citri Cladosporium subuliforme Secondary metabolites Entomopathogenic fungus |
| title | Novel isolate of Cladosporium subuliforme and its potential to control Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae) |
| title_full | Novel isolate of Cladosporium subuliforme and its potential to control Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae) |
| title_fullStr | Novel isolate of Cladosporium subuliforme and its potential to control Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae) |
| title_full_unstemmed | Novel isolate of Cladosporium subuliforme and its potential to control Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae) |
| title_short | Novel isolate of Cladosporium subuliforme and its potential to control Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae) |
| title_sort | novel isolate of cladosporium subuliforme and its potential to control asian citrus psyllid diaphorina citri kuwayama hemiptera liviidae |
| topic | Biocontrol Diaphorina citri Cladosporium subuliforme Secondary metabolites Entomopathogenic fungus |
| url | https://doi.org/10.1186/s41938-023-00685-0 |
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