Correlative STED and Atomic Force Microscopy on Live Astrocytes Reveals Plasticity of Cytoskeletal Structure and Membrane Physical Properties during Polarized Migration

Correlative STED and Atomic Force Microscopy on Live Astrocytes Reveals Plasticity of Cytoskeletal Structure and Membrane Physical Properties during Polarized Migration

The plasticity of the cytoskeleton architecture and membrane properties is important for the establishment of cell polarity, adhesion and migration. Here, we present a method which combines stimulated emission depletion (STED) super-resolution imaging and atomic force microscopy (AFM) to correlate c...

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Bibliographic Details
Main Authors: Nathalie Rouach, Clemens F. Kaminski, Nathan Curry, Grégory Ghézali, Gabriele S. Kaminski Schierle
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-04-01
Series:Frontiers in Cellular Neuroscience
Subjects:
astrocytes
migration
protrusions
membrane physical properties
cytoskeleton
atomic force microscopy
Online Access:http://journal.frontiersin.org/article/10.3389/fncel.2017.00104/full

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