Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy.
Fast, label-free, high-resolution, three-dimensional imaging platforms are crucial for high-throughput in vivo time-lapse studies of the anatomy of Caenorhabditis elegans, one of the most commonly used model organisms in biomedical research. Despite the needs, methods combining all these characteris...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5519216?pdf=render |
| _version_ | 1818683407811477504 |
|---|---|
| author | Séverine Coquoz Paul J Marchand Arno Bouwens Laurent Mouchiroud Vincenzo Sorrentino Daniel Szlag Johan Auwerx Theo Lasser |
| author_facet | Séverine Coquoz Paul J Marchand Arno Bouwens Laurent Mouchiroud Vincenzo Sorrentino Daniel Szlag Johan Auwerx Theo Lasser |
| author_sort | Séverine Coquoz |
| collection | DOAJ |
| description | Fast, label-free, high-resolution, three-dimensional imaging platforms are crucial for high-throughput in vivo time-lapse studies of the anatomy of Caenorhabditis elegans, one of the most commonly used model organisms in biomedical research. Despite the needs, methods combining all these characteristics have been lacking. Here, we present label-free imaging of live Caenorhabditis elegans with three-dimensional sub-micrometer resolution using visible optical coherence microscopy (visOCM). visOCM is a versatile optical imaging method which we introduced recently for tomography of cell cultures and tissue samples. Our method is based on Fourier domain optical coherence tomography, an interferometric technique that provides three-dimensional images with high sensitivity, high acquisition rate and micrometer-scale resolution. By operating in the visible wavelength range and using a high NA objective, visOCM attains lateral and axial resolutions below 1 μm. Additionally, we use a Bessel illumination offering an extended depth of field of approximately 40 μm. We demonstrate that visOCM's imaging properties allow rapid imaging of full sized living Caenorhabditis elegans down to the sub-cellular level. Our system opens the door to many applications such as the study of phenotypic changes related to developmental or ageing processes. |
| first_indexed | 2024-12-17T10:34:15Z |
| format | Article |
| id | doaj.art-290a12ddfd954f8aa2570b14651c79e7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T10:34:15Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-290a12ddfd954f8aa2570b14651c79e72022-12-21T21:52:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018167610.1371/journal.pone.0181676Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy.Séverine CoquozPaul J MarchandArno BouwensLaurent MouchiroudVincenzo SorrentinoDaniel SzlagJohan AuwerxTheo LasserFast, label-free, high-resolution, three-dimensional imaging platforms are crucial for high-throughput in vivo time-lapse studies of the anatomy of Caenorhabditis elegans, one of the most commonly used model organisms in biomedical research. Despite the needs, methods combining all these characteristics have been lacking. Here, we present label-free imaging of live Caenorhabditis elegans with three-dimensional sub-micrometer resolution using visible optical coherence microscopy (visOCM). visOCM is a versatile optical imaging method which we introduced recently for tomography of cell cultures and tissue samples. Our method is based on Fourier domain optical coherence tomography, an interferometric technique that provides three-dimensional images with high sensitivity, high acquisition rate and micrometer-scale resolution. By operating in the visible wavelength range and using a high NA objective, visOCM attains lateral and axial resolutions below 1 μm. Additionally, we use a Bessel illumination offering an extended depth of field of approximately 40 μm. We demonstrate that visOCM's imaging properties allow rapid imaging of full sized living Caenorhabditis elegans down to the sub-cellular level. Our system opens the door to many applications such as the study of phenotypic changes related to developmental or ageing processes.http://europepmc.org/articles/PMC5519216?pdf=render |
| spellingShingle | Séverine Coquoz Paul J Marchand Arno Bouwens Laurent Mouchiroud Vincenzo Sorrentino Daniel Szlag Johan Auwerx Theo Lasser Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy. PLoS ONE |
| title | Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy. |
| title_full | Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy. |
| title_fullStr | Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy. |
| title_full_unstemmed | Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy. |
| title_short | Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy. |
| title_sort | label free three dimensional imaging of caenorhabditis elegans with visible optical coherence microscopy |
| url | http://europepmc.org/articles/PMC5519216?pdf=render |
| work_keys_str_mv | AT severinecoquoz labelfreethreedimensionalimagingofcaenorhabditiseleganswithvisibleopticalcoherencemicroscopy AT pauljmarchand labelfreethreedimensionalimagingofcaenorhabditiseleganswithvisibleopticalcoherencemicroscopy AT arnobouwens labelfreethreedimensionalimagingofcaenorhabditiseleganswithvisibleopticalcoherencemicroscopy AT laurentmouchiroud labelfreethreedimensionalimagingofcaenorhabditiseleganswithvisibleopticalcoherencemicroscopy AT vincenzosorrentino labelfreethreedimensionalimagingofcaenorhabditiseleganswithvisibleopticalcoherencemicroscopy AT danielszlag labelfreethreedimensionalimagingofcaenorhabditiseleganswithvisibleopticalcoherencemicroscopy AT johanauwerx labelfreethreedimensionalimagingofcaenorhabditiseleganswithvisibleopticalcoherencemicroscopy AT theolasser labelfreethreedimensionalimagingofcaenorhabditiseleganswithvisibleopticalcoherencemicroscopy |