Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses
Summary: We describe a protocol for identifying bacteria-derived lipid metabolites produced in the guts using antibiotic-treated mice, liquid chromatography tandem mass spectrometry-based lipidomics, and feature-based molecular spectrum networking (FBMN). Untargeted lipidomics using the MS-DIAL 4 pr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721001994 |
| _version_ | 1818449158903693312 |
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| author | Nobuyuki Okahashi Masahiro Ueda Shu Yasuda Hiroshi Tsugawa Makoto Arita |
| author_facet | Nobuyuki Okahashi Masahiro Ueda Shu Yasuda Hiroshi Tsugawa Makoto Arita |
| author_sort | Nobuyuki Okahashi |
| collection | DOAJ |
| description | Summary: We describe a protocol for identifying bacteria-derived lipid metabolites produced in the guts using antibiotic-treated mice, liquid chromatography tandem mass spectrometry-based lipidomics, and feature-based molecular spectrum networking (FBMN). Untargeted lipidomics using the MS-DIAL 4 program provides information on known and unknown complex lipid molecules. The FBMN technique clusters similar MS2 spectra, facilitating the identification of bacterial lipids. Targeted analysis was used as a complementary method to cover oxylipins. Here, we provide details for targeted and untargeted analyses.For complete details on the use and execution of this protocol, please refer to Yasuda et al. (2020). |
| first_indexed | 2024-12-14T20:30:58Z |
| format | Article |
| id | doaj.art-290c041fa3404b84a4fae6224d048033 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-14T20:30:58Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-290c041fa3404b84a4fae6224d0480332022-12-21T22:48:32ZengElsevierSTAR Protocols2666-16672021-06-0122100492Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analysesNobuyuki Okahashi0Masahiro Ueda1Shu Yasuda2Hiroshi Tsugawa3Makoto Arita4RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan; Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Suita, Osaka 565-0871, JapanRIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan; JSR-Keio University Medical and Chemical Innovation Center, Shinjuku-ku, Tokyo 160-0016, JapanRIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, JapanRIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan; RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan; Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa 230-0045, JapanRIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan; Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa 230-0045, Japan; Division of Physiological Chemistry and Metabolism, Graduate School of Pharmaceutical Sciences, Keio University, Minato-ku, Tokyo 105-8512, Japan; Corresponding authorSummary: We describe a protocol for identifying bacteria-derived lipid metabolites produced in the guts using antibiotic-treated mice, liquid chromatography tandem mass spectrometry-based lipidomics, and feature-based molecular spectrum networking (FBMN). Untargeted lipidomics using the MS-DIAL 4 program provides information on known and unknown complex lipid molecules. The FBMN technique clusters similar MS2 spectra, facilitating the identification of bacterial lipids. Targeted analysis was used as a complementary method to cover oxylipins. Here, we provide details for targeted and untargeted analyses.For complete details on the use and execution of this protocol, please refer to Yasuda et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721001994ImmunologyMetabolomicsMicrobiologyMass Spectrometry |
| spellingShingle | Nobuyuki Okahashi Masahiro Ueda Shu Yasuda Hiroshi Tsugawa Makoto Arita Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses STAR Protocols Immunology Metabolomics Microbiology Mass Spectrometry |
| title | Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses |
| title_full | Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses |
| title_fullStr | Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses |
| title_full_unstemmed | Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses |
| title_short | Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses |
| title_sort | global profiling of gut microbiota associated lipid metabolites in antibiotic treated mice by lc ms ms based analyses |
| topic | Immunology Metabolomics Microbiology Mass Spectrometry |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721001994 |
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