Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses

Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses

Summary: We describe a protocol for identifying bacteria-derived lipid metabolites produced in the guts using antibiotic-treated mice, liquid chromatography tandem mass spectrometry-based lipidomics, and feature-based molecular spectrum networking (FBMN). Untargeted lipidomics using the MS-DIAL 4 pr...

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Bibliographic Details
Main Authors: Nobuyuki Okahashi, Masahiro Ueda, Shu Yasuda, Hiroshi Tsugawa, Makoto Arita
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Immunology
Metabolomics
Microbiology
Mass Spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001994

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