Association of SNP (RS4880) in SOD-2 Gene with Diabetic Nephropathy
Objective: To find an association between SNP (rs4880) in SOD-2 gene with Diabetic Nephropathy. Study Design: Case Control Study. Place & Duration of study: Department of Biochemistry and Molecular Biology, Army Medical College Rawalpindi and Armed Forces Institute of Urology, Rawalpindi...
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| Format: | Article |
| Language: | English |
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Army Medical College Rawalpindi
2023-10-01
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| Series: | Pakistan Armed Forces Medical Journal |
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| Online Access: | https://www.pafmj.org/PAFMJ/article/view/10578 |
| _version_ | 1797340996733763584 |
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| author | Sana Umar Javad Yousaf Amir Rashid Asifa Majeed Syed Zubair Hussain Shah |
| author_facet | Sana Umar Javad Yousaf Amir Rashid Asifa Majeed Syed Zubair Hussain Shah |
| author_sort | Sana Umar |
| collection | DOAJ |
| description |
Objective: To find an association between SNP (rs4880) in SOD-2 gene with Diabetic Nephropathy.
Study Design: Case Control Study.
Place & Duration of study: Department of Biochemistry and Molecular Biology, Army Medical College Rawalpindi and
Armed Forces Institute of Urology, Rawalpindi Pakistan, from Nov 2020 to Mar 2021.
Methodology: A total of 60 people were enrolled including 30 healthy individuals and 30 patients of diabetic nephropathy.
Both males and females aged 35-85 years were enrolled. Baseline biochemical tests were performed to ascertain diagnosis and exclude comorbidities. DNA was extracted using kit method and sequence of interest was amplified using PCR. Amplified product of genomic DNA of each individual was subjected to restriction fragment length polymorphism (RFLP) analysis. Data was analyzed using SPSS version 22.
Results: The frequency of TT genotype (40%) was higher among type 2 Diabetes Mellitus (T2DM) patients with nephropathy than CT (33.3%) and CC (26.6%) genotypes however the odds ratio for TT with 95% confidence interval (CI) was 2.53(0.69- 9.25) with p=0.35 which is statistically non-significant. The association value for genotypes was 1.29 (p-value=0.52) hence not significant.
Conclusion: There was no significant association present between SNP (rs4880) in SOD-2 gene which causes Val16Ala
polymorphism in superoxide dismutase 2 gene, with nephropathy in type 2 Diabetes Mellitus (T2DM) patients.
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| first_indexed | 2024-03-08T10:11:30Z |
| format | Article |
| id | doaj.art-291c3d471aaa4f07913f91b7537bc1e6 |
| institution | Directory Open Access Journal |
| issn | 0030-9648 2411-8842 |
| language | English |
| last_indexed | 2024-03-08T10:11:30Z |
| publishDate | 2023-10-01 |
| publisher | Army Medical College Rawalpindi |
| record_format | Article |
| series | Pakistan Armed Forces Medical Journal |
| spelling | doaj.art-291c3d471aaa4f07913f91b7537bc1e62024-01-29T08:26:24ZengArmy Medical College RawalpindiPakistan Armed Forces Medical Journal0030-96482411-88422023-10-0173510.51253/pafmj.v73i5.10578Association of SNP (RS4880) in SOD-2 Gene with Diabetic NephropathySana Umar0Javad Yousaf1Amir Rashid2Asifa Majeed3Syed Zubair Hussain Shah4Department of Biochemistry, Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanCombined Military Hospita, Multan Institute of Medical Sciences, Multan PakistanDepartment of Biochemistry, Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanDepartment of Biochemistry, Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanDepartment of Biochemistry, Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan Objective: To find an association between SNP (rs4880) in SOD-2 gene with Diabetic Nephropathy. Study Design: Case Control Study. Place & Duration of study: Department of Biochemistry and Molecular Biology, Army Medical College Rawalpindi and Armed Forces Institute of Urology, Rawalpindi Pakistan, from Nov 2020 to Mar 2021. Methodology: A total of 60 people were enrolled including 30 healthy individuals and 30 patients of diabetic nephropathy. Both males and females aged 35-85 years were enrolled. Baseline biochemical tests were performed to ascertain diagnosis and exclude comorbidities. DNA was extracted using kit method and sequence of interest was amplified using PCR. Amplified product of genomic DNA of each individual was subjected to restriction fragment length polymorphism (RFLP) analysis. Data was analyzed using SPSS version 22. Results: The frequency of TT genotype (40%) was higher among type 2 Diabetes Mellitus (T2DM) patients with nephropathy than CT (33.3%) and CC (26.6%) genotypes however the odds ratio for TT with 95% confidence interval (CI) was 2.53(0.69- 9.25) with p=0.35 which is statistically non-significant. The association value for genotypes was 1.29 (p-value=0.52) hence not significant. Conclusion: There was no significant association present between SNP (rs4880) in SOD-2 gene which causes Val16Ala polymorphism in superoxide dismutase 2 gene, with nephropathy in type 2 Diabetes Mellitus (T2DM) patients. https://www.pafmj.org/PAFMJ/article/view/10578Diabetic nephropathysuperoxide dismutase 2 geneSNP rs4880Val16Ala polymorphism |
| spellingShingle | Sana Umar Javad Yousaf Amir Rashid Asifa Majeed Syed Zubair Hussain Shah Association of SNP (RS4880) in SOD-2 Gene with Diabetic Nephropathy Pakistan Armed Forces Medical Journal Diabetic nephropathy superoxide dismutase 2 gene SNP rs4880 Val16Ala polymorphism |
| title | Association of SNP (RS4880) in SOD-2 Gene with Diabetic Nephropathy |
| title_full | Association of SNP (RS4880) in SOD-2 Gene with Diabetic Nephropathy |
| title_fullStr | Association of SNP (RS4880) in SOD-2 Gene with Diabetic Nephropathy |
| title_full_unstemmed | Association of SNP (RS4880) in SOD-2 Gene with Diabetic Nephropathy |
| title_short | Association of SNP (RS4880) in SOD-2 Gene with Diabetic Nephropathy |
| title_sort | association of snp rs4880 in sod 2 gene with diabetic nephropathy |
| topic | Diabetic nephropathy superoxide dismutase 2 gene SNP rs4880 Val16Ala polymorphism |
| url | https://www.pafmj.org/PAFMJ/article/view/10578 |
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