Genetic Analysis of S5-Interacting Genes Regulating Hybrid Sterility in Rice
Abstract Background Asian cultivated rice (Oryza sativa L.) comprises two subspecies, O. sativa subsp. indica and subsp. japonica, and the hybrids between them display strong heterosis. However, hybrid sterility (HS) limits practical use of the heterosis between these two subspecies. S5 is a major-e...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-01-01
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| Series: | Rice |
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| Online Access: | https://doi.org/10.1186/s12284-020-00452-x |
| _version_ | 1818575443126648832 |
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| author | Jianglei Rao Xing Wang Zhongquan Cai Yourong Fan Jiangyi Yang |
| author_facet | Jianglei Rao Xing Wang Zhongquan Cai Yourong Fan Jiangyi Yang |
| author_sort | Jianglei Rao |
| collection | DOAJ |
| description | Abstract Background Asian cultivated rice (Oryza sativa L.) comprises two subspecies, O. sativa subsp. indica and subsp. japonica, and the hybrids between them display strong heterosis. However, hybrid sterility (HS) limits practical use of the heterosis between these two subspecies. S5 is a major-effect locus controlling the HS of female gametes in rice, consisting of three closely-linked genes ORF3, ORF4 and ORF5 that act as a killer-protector system. The HS effects of S5 are inconsistent for different genetic backgrounds, indicating the existence of interacting genes within the genome. Results In the present study, the S5-interacting genes (SIG) and their effects on HS were analyzed by studying the hybrid progeny between an indica rice, Dular (DL) and a japonica rice, Balilla ORF5+ (BL ORF5+), with a transgenic ORF5+ allele. Four interacting quantitative trait loci (QTL): qSIG3.1, qSIG3.2, qSIG6.1, and qSIG12.1, were genetically mapped. To analyze the effect of each interacting locus, four near-isogenic lines (NILs) were developed. The effect of each specific locus was investigated while the other three loci were kept DL homozygous (DL/DL). Of the four loci, qSIG3.1 was the SIG with the greatest effects in which the DL allele was completely dominant. Furthermore, the DL allele displayed incomplete dominance at qSIG3.2, qSIG6.1, and qSIG12.1. qSIG3.1 will be the first choice for further fine-mapping. Conclusions Four S5-interacting QTL were identified by genetic mapping and the effect of each locus was analyzed using advanced backcrossed NILs. The present study will facilitate elucidation of the molecular mechanism of rice HS caused by S5. Additionally, it would provide the basis to explore the origin and differentiation of cultivated rice, having practical significance for inter-subspecific hybrid rice breeding programs. |
| first_indexed | 2024-12-15T00:40:21Z |
| format | Article |
| id | doaj.art-291e73e1418f4e5d8d0d00fe1c4f14cc |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-12-15T00:40:21Z |
| publishDate | 2021-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-291e73e1418f4e5d8d0d00fe1c4f14cc2022-12-21T22:41:40ZengSpringerOpenRice1939-84251939-84332021-01-011411910.1186/s12284-020-00452-xGenetic Analysis of S5-Interacting Genes Regulating Hybrid Sterility in RiceJianglei Rao0Xing Wang1Zhongquan Cai2Yourong Fan3Jiangyi Yang4State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi UniversityAbstract Background Asian cultivated rice (Oryza sativa L.) comprises two subspecies, O. sativa subsp. indica and subsp. japonica, and the hybrids between them display strong heterosis. However, hybrid sterility (HS) limits practical use of the heterosis between these two subspecies. S5 is a major-effect locus controlling the HS of female gametes in rice, consisting of three closely-linked genes ORF3, ORF4 and ORF5 that act as a killer-protector system. The HS effects of S5 are inconsistent for different genetic backgrounds, indicating the existence of interacting genes within the genome. Results In the present study, the S5-interacting genes (SIG) and their effects on HS were analyzed by studying the hybrid progeny between an indica rice, Dular (DL) and a japonica rice, Balilla ORF5+ (BL ORF5+), with a transgenic ORF5+ allele. Four interacting quantitative trait loci (QTL): qSIG3.1, qSIG3.2, qSIG6.1, and qSIG12.1, were genetically mapped. To analyze the effect of each interacting locus, four near-isogenic lines (NILs) were developed. The effect of each specific locus was investigated while the other three loci were kept DL homozygous (DL/DL). Of the four loci, qSIG3.1 was the SIG with the greatest effects in which the DL allele was completely dominant. Furthermore, the DL allele displayed incomplete dominance at qSIG3.2, qSIG6.1, and qSIG12.1. qSIG3.1 will be the first choice for further fine-mapping. Conclusions Four S5-interacting QTL were identified by genetic mapping and the effect of each locus was analyzed using advanced backcrossed NILs. The present study will facilitate elucidation of the molecular mechanism of rice HS caused by S5. Additionally, it would provide the basis to explore the origin and differentiation of cultivated rice, having practical significance for inter-subspecific hybrid rice breeding programs.https://doi.org/10.1186/s12284-020-00452-xHybrid incompatibilityWide compatibilityReproductive isolationSpikelet fertilityQuantitative trait loci mapping |
| spellingShingle | Jianglei Rao Xing Wang Zhongquan Cai Yourong Fan Jiangyi Yang Genetic Analysis of S5-Interacting Genes Regulating Hybrid Sterility in Rice Rice Hybrid incompatibility Wide compatibility Reproductive isolation Spikelet fertility Quantitative trait loci mapping |
| title | Genetic Analysis of S5-Interacting Genes Regulating Hybrid Sterility in Rice |
| title_full | Genetic Analysis of S5-Interacting Genes Regulating Hybrid Sterility in Rice |
| title_fullStr | Genetic Analysis of S5-Interacting Genes Regulating Hybrid Sterility in Rice |
| title_full_unstemmed | Genetic Analysis of S5-Interacting Genes Regulating Hybrid Sterility in Rice |
| title_short | Genetic Analysis of S5-Interacting Genes Regulating Hybrid Sterility in Rice |
| title_sort | genetic analysis of s5 interacting genes regulating hybrid sterility in rice |
| topic | Hybrid incompatibility Wide compatibility Reproductive isolation Spikelet fertility Quantitative trait loci mapping |
| url | https://doi.org/10.1186/s12284-020-00452-x |
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