Emergence of optrA-Mediated Linezolid Resistance in Enterococcus faecium: A Molecular Investigation in a Tertiary Hospital of Southwest China from 2014–2018
Miao Yi,1 Jiaqi Zou,2 Jinxin Zhao,1 Yu Tang,3 Yaling Yuan,1 Bingxue Yang,1 Jinzhu Huang,1 Peiwen Xia,1 Yun Xia1 1Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China; 2Department of Clinical Laboratory, Chengdu Women’...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-01-01
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| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/emergence-of-optra-mediated-linezolid-resistance-in-enterococcus-faeci-peer-reviewed-fulltext-article-IDR |
| _version_ | 1819001313255489536 |
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| author | Yi M Zou J Zhao J Tang Y Yuan Y Yang B Huang J Xia P Xia Y |
| author_facet | Yi M Zou J Zhao J Tang Y Yuan Y Yang B Huang J Xia P Xia Y |
| author_sort | Yi M |
| collection | DOAJ |
| description | Miao Yi,1 Jiaqi Zou,2 Jinxin Zhao,1 Yu Tang,3 Yaling Yuan,1 Bingxue Yang,1 Jinzhu Huang,1 Peiwen Xia,1 Yun Xia1 1Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China; 2Department of Clinical Laboratory, Chengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, People’s Republic of China; 3Department of Laboratory Medicine, Chongqing University Three Gorges Hospital, Chongqing, People’s Republic of ChinaCorrespondence: Yun XiaDepartment of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing, 400016, People’s Republic of ChinaTel +86-23-89012742Fax +86-23-89012513Email xiayun12cn@aliyun.comPurpose: To investigate the potential mechanism and molecular characteristics of linezolid-non-sensitive Enterococcus faecium from a tertiary hospital in southwest China and characterize the relevant plasmids.Patients and Methods: Linezolid-non-sensitive Enterococcus faecium (LNSEFM) isolates collected from January 2014 to December 2018 were screened for resistant genes 23s rRNA, rplC, rplD, rplV, optrA, cfr, poxtA, by PCR. Molecular epidemiological analysis was performed by multilocus sequence typing (MLST). The optrA-and-poxtA co-harboring strain EFM_7150 was subjected to the whole genome sequencing (WGS) by Illumina HiSeq and Oxford Nanopore MinION.Results: A total of 15 LNSEFM with linezolid MICs ranging from 4 to 16 mg/L were identified. About 66.7% (10/15) of isolates were linezolid-resistant. About 46.7% (7/15) of strains were positive for optrA. Two types of optrA variants (P and EYDNDM) were identified. About 13.3% (2/15) of isolates had poxtA. 1 harbored a L22 protein alteration (Ser77Thr). One isolate coharbored optrA (EYDNDM variant) and poxtA. There was no mutation in the gene that encoded the ribosomal protein L3/L4 or the domain V of 23S rRNA. No cfr gene was detected. Based on WGS data, optrA was associated with Tn558 inserted to radC gene and poxtA was flanked by IS1216E.Conclusion: OptrA is primary mechanism in linezolid-resistant Enterococcus faecium. This is the first report ofoptrA variants P and EYDNDM identified in Enterococcus faecium and optrA-and-poxtA co-harboring Enterococcus faecium clinically in southwest China. Besides, Tn558 and IS1216Es may play an important role in the dissemination of optrA and poxtA, respectively. The findings revealed the potential threat to nosocomial infection by optrA and coexistence of optrA and poxtA in Enterococcus faecium. Thus, clinical surveillance of linezolid-resistant Enterococcus is urgently needed.Keywords: optrA, poxtA, linezolid-non-sensitive Enterococcus faecium, WGS |
| first_indexed | 2024-12-20T22:47:13Z |
| format | Article |
| id | doaj.art-291e806943d541eda6ad40ac1891ab55 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-20T22:47:13Z |
| publishDate | 2022-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-291e806943d541eda6ad40ac1891ab552022-12-21T19:24:20ZengDove Medical PressInfection and Drug Resistance1178-69732022-01-01Volume 15132072005Emergence of optrA-Mediated Linezolid Resistance in Enterococcus faecium: A Molecular Investigation in a Tertiary Hospital of Southwest China from 2014–2018Yi MZou JZhao JTang YYuan YYang BHuang JXia PXia YMiao Yi,1 Jiaqi Zou,2 Jinxin Zhao,1 Yu Tang,3 Yaling Yuan,1 Bingxue Yang,1 Jinzhu Huang,1 Peiwen Xia,1 Yun Xia1 1Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China; 2Department of Clinical Laboratory, Chengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, People’s Republic of China; 3Department of Laboratory Medicine, Chongqing University Three Gorges Hospital, Chongqing, People’s Republic of ChinaCorrespondence: Yun XiaDepartment of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing, 400016, People’s Republic of ChinaTel +86-23-89012742Fax +86-23-89012513Email xiayun12cn@aliyun.comPurpose: To investigate the potential mechanism and molecular characteristics of linezolid-non-sensitive Enterococcus faecium from a tertiary hospital in southwest China and characterize the relevant plasmids.Patients and Methods: Linezolid-non-sensitive Enterococcus faecium (LNSEFM) isolates collected from January 2014 to December 2018 were screened for resistant genes 23s rRNA, rplC, rplD, rplV, optrA, cfr, poxtA, by PCR. Molecular epidemiological analysis was performed by multilocus sequence typing (MLST). The optrA-and-poxtA co-harboring strain EFM_7150 was subjected to the whole genome sequencing (WGS) by Illumina HiSeq and Oxford Nanopore MinION.Results: A total of 15 LNSEFM with linezolid MICs ranging from 4 to 16 mg/L were identified. About 66.7% (10/15) of isolates were linezolid-resistant. About 46.7% (7/15) of strains were positive for optrA. Two types of optrA variants (P and EYDNDM) were identified. About 13.3% (2/15) of isolates had poxtA. 1 harbored a L22 protein alteration (Ser77Thr). One isolate coharbored optrA (EYDNDM variant) and poxtA. There was no mutation in the gene that encoded the ribosomal protein L3/L4 or the domain V of 23S rRNA. No cfr gene was detected. Based on WGS data, optrA was associated with Tn558 inserted to radC gene and poxtA was flanked by IS1216E.Conclusion: OptrA is primary mechanism in linezolid-resistant Enterococcus faecium. This is the first report ofoptrA variants P and EYDNDM identified in Enterococcus faecium and optrA-and-poxtA co-harboring Enterococcus faecium clinically in southwest China. Besides, Tn558 and IS1216Es may play an important role in the dissemination of optrA and poxtA, respectively. The findings revealed the potential threat to nosocomial infection by optrA and coexistence of optrA and poxtA in Enterococcus faecium. Thus, clinical surveillance of linezolid-resistant Enterococcus is urgently needed.Keywords: optrA, poxtA, linezolid-non-sensitive Enterococcus faecium, WGShttps://www.dovepress.com/emergence-of-optra-mediated-linezolid-resistance-in-enterococcus-faeci-peer-reviewed-fulltext-article-IDRoptrapoxtalinezolid-non-sensitive enterococcus faeciumwgs |
| spellingShingle | Yi M Zou J Zhao J Tang Y Yuan Y Yang B Huang J Xia P Xia Y Emergence of optrA-Mediated Linezolid Resistance in Enterococcus faecium: A Molecular Investigation in a Tertiary Hospital of Southwest China from 2014–2018 Infection and Drug Resistance optra poxta linezolid-non-sensitive enterococcus faecium wgs |
| title | Emergence of optrA-Mediated Linezolid Resistance in Enterococcus faecium: A Molecular Investigation in a Tertiary Hospital of Southwest China from 2014–2018 |
| title_full | Emergence of optrA-Mediated Linezolid Resistance in Enterococcus faecium: A Molecular Investigation in a Tertiary Hospital of Southwest China from 2014–2018 |
| title_fullStr | Emergence of optrA-Mediated Linezolid Resistance in Enterococcus faecium: A Molecular Investigation in a Tertiary Hospital of Southwest China from 2014–2018 |
| title_full_unstemmed | Emergence of optrA-Mediated Linezolid Resistance in Enterococcus faecium: A Molecular Investigation in a Tertiary Hospital of Southwest China from 2014–2018 |
| title_short | Emergence of optrA-Mediated Linezolid Resistance in Enterococcus faecium: A Molecular Investigation in a Tertiary Hospital of Southwest China from 2014–2018 |
| title_sort | emergence of optra mediated linezolid resistance in enterococcus faecium a molecular investigation in a tertiary hospital of southwest china from 2014 ndash 2018 |
| topic | optra poxta linezolid-non-sensitive enterococcus faecium wgs |
| url | https://www.dovepress.com/emergence-of-optra-mediated-linezolid-resistance-in-enterococcus-faeci-peer-reviewed-fulltext-article-IDR |
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