The Glutathione Peroxidase Gene Family in <i>Nitraria sibirica</i>: Genome-Wide Identification, Classification, and Gene Expression Analysis under Stress Conditions
Plant glutathione peroxidases (GPXs) are the main enzymes in the antioxidant defense system that sustain H<sub>2</sub>O<sub>2</sub> homeostasis and normalize plant reaction to abiotic stress conditions. However, the genome-wide identification of the <i>GPX</i> gen...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/4/950 |
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| author | Ziming Lian Jingbo Zhang Zhaodong Hao Liming Zhu Yuxin Liu Hao Fang Ye Lu Xinle Li Jisen Shi Jinhui Chen Tielong Cheng |
| author_facet | Ziming Lian Jingbo Zhang Zhaodong Hao Liming Zhu Yuxin Liu Hao Fang Ye Lu Xinle Li Jisen Shi Jinhui Chen Tielong Cheng |
| author_sort | Ziming Lian |
| collection | DOAJ |
| description | Plant glutathione peroxidases (GPXs) are the main enzymes in the antioxidant defense system that sustain H<sub>2</sub>O<sub>2</sub> homeostasis and normalize plant reaction to abiotic stress conditions. However, the genome-wide identification of the <i>GPX</i> gene family and its responses to environmental stresses, especially salt stress, in <i>Nitraria sibirica</i>, which is a shrub that can survive in saline environments, has not yet been reported. Here, we first report the genome-wide analysis of the <i>GPX</i> gene family in <i>N. sibirica</i>, leading to a total of seven <i>NsGPX</i> genes that are distributed on six of the twelve chromosomes. Phylogenetic analysis showed that <i>NsGPX</i> genes were grouped into four major groups (Group I-IV). Three types of cis-acting elements were identified in the <i>NsGPX</i> promoters, mainly related to hormones and stress response. The quantitative real-time PCR (qRT-PCR) analysis indicated that <i>NsGPX1</i> and <i>NsGPX3</i> were significantly up-regulated in stem and leaf, while <i>NsGPX7</i> transcriptionally in root in response to salt stress. The current study identified a total seven NsGPX genes in <i>N. sibirica</i> via genome-wide analysis, and discovered that NsGPXs may play an important role in response to salt stress. Taken together, our findings provide a basis for further functional studies of <i>NsGPX</i> genes, especially in regarding to the resistance to salt stress of this halophyte plant <i>N. sibirica</i>, eventually aid in the discovery of new methods to restore overtly saline soil. |
| first_indexed | 2024-03-11T04:58:47Z |
| format | Article |
| id | doaj.art-2920ab1b82fc45388e1db2c1128c866d |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T04:58:47Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-2920ab1b82fc45388e1db2c1128c866d2023-11-17T19:24:56ZengMDPI AGGenes2073-44252023-04-0114495010.3390/genes14040950The Glutathione Peroxidase Gene Family in <i>Nitraria sibirica</i>: Genome-Wide Identification, Classification, and Gene Expression Analysis under Stress ConditionsZiming Lian0Jingbo Zhang1Zhaodong Hao2Liming Zhu3Yuxin Liu4Hao Fang5Ye Lu6Xinle Li7Jisen Shi8Jinhui Chen9Tielong Cheng10State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaExperimental Center of Desert Forestry, Chinese Academy of Forestry, Dengkou 015200, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaExperimental Center of Desert Forestry, Chinese Academy of Forestry, Dengkou 015200, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaPlant glutathione peroxidases (GPXs) are the main enzymes in the antioxidant defense system that sustain H<sub>2</sub>O<sub>2</sub> homeostasis and normalize plant reaction to abiotic stress conditions. However, the genome-wide identification of the <i>GPX</i> gene family and its responses to environmental stresses, especially salt stress, in <i>Nitraria sibirica</i>, which is a shrub that can survive in saline environments, has not yet been reported. Here, we first report the genome-wide analysis of the <i>GPX</i> gene family in <i>N. sibirica</i>, leading to a total of seven <i>NsGPX</i> genes that are distributed on six of the twelve chromosomes. Phylogenetic analysis showed that <i>NsGPX</i> genes were grouped into four major groups (Group I-IV). Three types of cis-acting elements were identified in the <i>NsGPX</i> promoters, mainly related to hormones and stress response. The quantitative real-time PCR (qRT-PCR) analysis indicated that <i>NsGPX1</i> and <i>NsGPX3</i> were significantly up-regulated in stem and leaf, while <i>NsGPX7</i> transcriptionally in root in response to salt stress. The current study identified a total seven NsGPX genes in <i>N. sibirica</i> via genome-wide analysis, and discovered that NsGPXs may play an important role in response to salt stress. Taken together, our findings provide a basis for further functional studies of <i>NsGPX</i> genes, especially in regarding to the resistance to salt stress of this halophyte plant <i>N. sibirica</i>, eventually aid in the discovery of new methods to restore overtly saline soil.https://www.mdpi.com/2073-4425/14/4/950<i>Nitraria sibirica</i>glutathione peroxidasesalt stressgenomicsgene expression |
| spellingShingle | Ziming Lian Jingbo Zhang Zhaodong Hao Liming Zhu Yuxin Liu Hao Fang Ye Lu Xinle Li Jisen Shi Jinhui Chen Tielong Cheng The Glutathione Peroxidase Gene Family in <i>Nitraria sibirica</i>: Genome-Wide Identification, Classification, and Gene Expression Analysis under Stress Conditions Genes <i>Nitraria sibirica</i> glutathione peroxidase salt stress genomics gene expression |
| title | The Glutathione Peroxidase Gene Family in <i>Nitraria sibirica</i>: Genome-Wide Identification, Classification, and Gene Expression Analysis under Stress Conditions |
| title_full | The Glutathione Peroxidase Gene Family in <i>Nitraria sibirica</i>: Genome-Wide Identification, Classification, and Gene Expression Analysis under Stress Conditions |
| title_fullStr | The Glutathione Peroxidase Gene Family in <i>Nitraria sibirica</i>: Genome-Wide Identification, Classification, and Gene Expression Analysis under Stress Conditions |
| title_full_unstemmed | The Glutathione Peroxidase Gene Family in <i>Nitraria sibirica</i>: Genome-Wide Identification, Classification, and Gene Expression Analysis under Stress Conditions |
| title_short | The Glutathione Peroxidase Gene Family in <i>Nitraria sibirica</i>: Genome-Wide Identification, Classification, and Gene Expression Analysis under Stress Conditions |
| title_sort | glutathione peroxidase gene family in i nitraria sibirica i genome wide identification classification and gene expression analysis under stress conditions |
| topic | <i>Nitraria sibirica</i> glutathione peroxidase salt stress genomics gene expression |
| url | https://www.mdpi.com/2073-4425/14/4/950 |
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