Development of Novel and Highly Specific ssDNA-Aptamer-Based Electrochemical Biosensor for Rapid Detection of Mercury (II) and Lead (II) Ions in Water
In this work, we report on the development of an electrochemical biosensor for high selectivity and rapid detection of Hg<sup>2+</sup> and Pb<sup>2+</sup> ions using DNA-based specific aptamer probes labeled with ferrocene (or methylene blue) and thiol groups at their 5&#...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-06-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/7/2/27 |
| _version_ | 1818286940454125568 |
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| author | Hisham Abu-Ali Alexei Nabok Thomas J. Smith |
| author_facet | Hisham Abu-Ali Alexei Nabok Thomas J. Smith |
| author_sort | Hisham Abu-Ali |
| collection | DOAJ |
| description | In this work, we report on the development of an electrochemical biosensor for high selectivity and rapid detection of Hg<sup>2+</sup> and Pb<sup>2+</sup> ions using DNA-based specific aptamer probes labeled with ferrocene (or methylene blue) and thiol groups at their 5′ and 3′ termini, respectively. Aptamers were immobilized onto the surface of screen-printed gold electrodes via the SH (thiol) groups, and then cyclic voltammetry and impedance spectra measurements were performed in buffer solutions with the addition of HgCl<sub>2</sub> and PbCl<sub>2</sub> salts at different concentrations. Changes in 3D conformation of aptamers, caused by binding their respective targets, e.g., Hg<sup>2+</sup> and Pb<sup>2+</sup> ions, were accompanied by an increase in the electron transfer between the redox label and the electrode. Accordingly, the presence of the above ions can be detected electrochemically. The detection of Hg<sup>2+</sup> and Pb<sup>2+</sup> ions in a wide range of concentrations as low as 0.1 ng/mL (or 0.1 ppb) was achieved. The study of the kinetics of aptamer/heavy metal ions binding gave the values of the affinity constants of approximately 9.10<sup>−7</sup> mol, which proved the high specificity of the aptamers used. |
| first_indexed | 2024-12-13T01:32:34Z |
| format | Article |
| id | doaj.art-29221395aadf4e55b683e1647dac638a |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-12-13T01:32:34Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-29221395aadf4e55b683e1647dac638a2022-12-22T00:03:57ZengMDPI AGChemosensors2227-90402019-06-01722710.3390/chemosensors7020027chemosensors7020027Development of Novel and Highly Specific ssDNA-Aptamer-Based Electrochemical Biosensor for Rapid Detection of Mercury (II) and Lead (II) Ions in WaterHisham Abu-Ali0Alexei Nabok1Thomas J. Smith2Material and Engineering Research Institute, Sheffield Hallam University, Sheffield S1 1WB, UKMaterial and Engineering Research Institute, Sheffield Hallam University, Sheffield S1 1WB, UKBiomolecular Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UKIn this work, we report on the development of an electrochemical biosensor for high selectivity and rapid detection of Hg<sup>2+</sup> and Pb<sup>2+</sup> ions using DNA-based specific aptamer probes labeled with ferrocene (or methylene blue) and thiol groups at their 5′ and 3′ termini, respectively. Aptamers were immobilized onto the surface of screen-printed gold electrodes via the SH (thiol) groups, and then cyclic voltammetry and impedance spectra measurements were performed in buffer solutions with the addition of HgCl<sub>2</sub> and PbCl<sub>2</sub> salts at different concentrations. Changes in 3D conformation of aptamers, caused by binding their respective targets, e.g., Hg<sup>2+</sup> and Pb<sup>2+</sup> ions, were accompanied by an increase in the electron transfer between the redox label and the electrode. Accordingly, the presence of the above ions can be detected electrochemically. The detection of Hg<sup>2+</sup> and Pb<sup>2+</sup> ions in a wide range of concentrations as low as 0.1 ng/mL (or 0.1 ppb) was achieved. The study of the kinetics of aptamer/heavy metal ions binding gave the values of the affinity constants of approximately 9.10<sup>−7</sup> mol, which proved the high specificity of the aptamers used.https://www.mdpi.com/2227-9040/7/2/27aptamer sensorselectrochemical sensorsheavy metal ionscyclic voltammetryimpedance spectroscopybinding kinetics |
| spellingShingle | Hisham Abu-Ali Alexei Nabok Thomas J. Smith Development of Novel and Highly Specific ssDNA-Aptamer-Based Electrochemical Biosensor for Rapid Detection of Mercury (II) and Lead (II) Ions in Water Chemosensors aptamer sensors electrochemical sensors heavy metal ions cyclic voltammetry impedance spectroscopy binding kinetics |
| title | Development of Novel and Highly Specific ssDNA-Aptamer-Based Electrochemical Biosensor for Rapid Detection of Mercury (II) and Lead (II) Ions in Water |
| title_full | Development of Novel and Highly Specific ssDNA-Aptamer-Based Electrochemical Biosensor for Rapid Detection of Mercury (II) and Lead (II) Ions in Water |
| title_fullStr | Development of Novel and Highly Specific ssDNA-Aptamer-Based Electrochemical Biosensor for Rapid Detection of Mercury (II) and Lead (II) Ions in Water |
| title_full_unstemmed | Development of Novel and Highly Specific ssDNA-Aptamer-Based Electrochemical Biosensor for Rapid Detection of Mercury (II) and Lead (II) Ions in Water |
| title_short | Development of Novel and Highly Specific ssDNA-Aptamer-Based Electrochemical Biosensor for Rapid Detection of Mercury (II) and Lead (II) Ions in Water |
| title_sort | development of novel and highly specific ssdna aptamer based electrochemical biosensor for rapid detection of mercury ii and lead ii ions in water |
| topic | aptamer sensors electrochemical sensors heavy metal ions cyclic voltammetry impedance spectroscopy binding kinetics |
| url | https://www.mdpi.com/2227-9040/7/2/27 |
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