Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals
The application of nanoparticles has experienced a vertiginous growth, but their interaction with food and medicinal plants in organisms, especially in the control of reproduction, remains unresolved. We examined the influence of copper nanoparticles supported on titania (CuNPs/TiO<sub>2</s...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/9/1859 |
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| author | Alexander V. Sirotkin Monika Radosová Adam Tarko Zuzana Fabova Iris Martín-García Francisco Alonso |
| author_facet | Alexander V. Sirotkin Monika Radosová Adam Tarko Zuzana Fabova Iris Martín-García Francisco Alonso |
| author_sort | Alexander V. Sirotkin |
| collection | DOAJ |
| description | The application of nanoparticles has experienced a vertiginous growth, but their interaction with food and medicinal plants in organisms, especially in the control of reproduction, remains unresolved. We examined the influence of copper nanoparticles supported on titania (CuNPs/TiO<sub>2</sub>), plant extracts (buckwheat (<i>Fagopyrum esculentum</i>) and vitex (<i>Vitex agnus-castus</i>)), phytochemicals (rutin and apigenin), and their combination with CuNPs/TiO<sub>2</sub> on ovarian cell functions, using cultured porcine ovarian granulosa cells. Cell viability, proliferation (PCNA accumulation), apoptosis (accumulation of bax), and hormones release (progesterone, testosterone, and 17<i>β</i>-estradiol) were analyzed by the Trypan blue test, quantitative immunocytochemistry, and ELISA, respectively. CuNPs/TiO<sub>2</sub> increased cell viability, proliferation, apoptosis, and testosterone but not progesterone release, and reduced the 17<i>β</i>-estradiol output. Plant extracts and components have similar stimulatory action on ovarian cell functions as CuNPs/TiO<sub>2</sub>, but abated the majority of the CuNPs/TiO<sub>2</sub> effects. This study concludes that (1) CuNPs/TiO<sub>2</sub> can directly stimulate ovarian cell functions, promoting ovarian cell proliferation, apoptosis, turnover, viability, and steroid hormones release; (2) the plants buckwheat and vitex, as well as rutin and apigenin, can promote some of these ovarian functions too; and (3) these plant additives mitigate the CuNPs/TiO<sub>2</sub>’s activity, something that must be considered when applied together. |
| first_indexed | 2024-03-10T16:16:16Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T16:16:16Z |
| publishDate | 2020-09-01 |
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| series | Nanomaterials |
| spelling | doaj.art-2923cf7deeb04791b9ec827de2f543972023-11-20T14:02:08ZengMDPI AGNanomaterials2079-49912020-09-01109185910.3390/nano10091859Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and PhytochemicalsAlexander V. Sirotkin0Monika Radosová1Adam Tarko2Zuzana Fabova3Iris Martín-García4Francisco Alonso5Department of Zoology and Anthropology, Constantine the Philosopher University, Tr. A Hlinku 1, 949 74 Nitra, SlovakiaDepartment of Zoology and Anthropology, Constantine the Philosopher University, Tr. A Hlinku 1, 949 74 Nitra, SlovakiaDepartment of Zoology and Anthropology, Constantine the Philosopher University, Tr. A Hlinku 1, 949 74 Nitra, SlovakiaDepartment of Zoology and Anthropology, Constantine the Philosopher University, Tr. A Hlinku 1, 949 74 Nitra, SlovakiaInstituto de Síntesis Orgánica (ISO) and Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Alicante, Apdo., 99, 03080 Alicante, SpainInstituto de Síntesis Orgánica (ISO) and Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Alicante, Apdo., 99, 03080 Alicante, SpainThe application of nanoparticles has experienced a vertiginous growth, but their interaction with food and medicinal plants in organisms, especially in the control of reproduction, remains unresolved. We examined the influence of copper nanoparticles supported on titania (CuNPs/TiO<sub>2</sub>), plant extracts (buckwheat (<i>Fagopyrum esculentum</i>) and vitex (<i>Vitex agnus-castus</i>)), phytochemicals (rutin and apigenin), and their combination with CuNPs/TiO<sub>2</sub> on ovarian cell functions, using cultured porcine ovarian granulosa cells. Cell viability, proliferation (PCNA accumulation), apoptosis (accumulation of bax), and hormones release (progesterone, testosterone, and 17<i>β</i>-estradiol) were analyzed by the Trypan blue test, quantitative immunocytochemistry, and ELISA, respectively. CuNPs/TiO<sub>2</sub> increased cell viability, proliferation, apoptosis, and testosterone but not progesterone release, and reduced the 17<i>β</i>-estradiol output. Plant extracts and components have similar stimulatory action on ovarian cell functions as CuNPs/TiO<sub>2</sub>, but abated the majority of the CuNPs/TiO<sub>2</sub> effects. This study concludes that (1) CuNPs/TiO<sub>2</sub> can directly stimulate ovarian cell functions, promoting ovarian cell proliferation, apoptosis, turnover, viability, and steroid hormones release; (2) the plants buckwheat and vitex, as well as rutin and apigenin, can promote some of these ovarian functions too; and (3) these plant additives mitigate the CuNPs/TiO<sub>2</sub>’s activity, something that must be considered when applied together.https://www.mdpi.com/2079-4991/10/9/1859apoptosiscopper nanoparticleshormonesbuckwheatvitexrutin |
| spellingShingle | Alexander V. Sirotkin Monika Radosová Adam Tarko Zuzana Fabova Iris Martín-García Francisco Alonso Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals Nanomaterials apoptosis copper nanoparticles hormones buckwheat vitex rutin |
| title | Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals |
| title_full | Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals |
| title_fullStr | Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals |
| title_full_unstemmed | Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals |
| title_short | Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals |
| title_sort | abatement of the stimulatory effect of copper nanoparticles supported on titania on ovarian cell functions by some plants and phytochemicals |
| topic | apoptosis copper nanoparticles hormones buckwheat vitex rutin |
| url | https://www.mdpi.com/2079-4991/10/9/1859 |
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