Pattern of Microorganisms Isolated from Flame Burn Wounds, and Their Trends of Susceptibility to Antibiotic During Past Three Years
Objective: To determine the prevalent bacteria causing wound infection and their trend of susceptibility to antibiotics over a period of last three years in flame burn cases. This is an observational descriptive study conducted at the Burns Center, Civil Hospital Karachi. Methods: All availabl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Dow University of Health Sciences
2013-04-01
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| Series: | Journal of the Dow University of Health Sciences |
| Subjects: | |
| Online Access: | http://www.jduhs.com/index.php/jduhs/article/view/71 |
| _version_ | 1811329690019823616 |
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| author | Ehmer Al-Ibran Sidra Meraj Mahwish Nasim Maria Khan Masood Hussain Rao |
| author_facet | Ehmer Al-Ibran Sidra Meraj Mahwish Nasim Maria Khan Masood Hussain Rao |
| author_sort | Ehmer Al-Ibran |
| collection | DOAJ |
| description | Objective:
To determine the prevalent bacteria causing wound infection and their trend of susceptibility to antibiotics over a period of last three years in flame burn cases. This is an observational descriptive study conducted at the Burns Center, Civil Hospital Karachi.
Methods:
All available wound swabs culture and sensitivity reports of flame burn victims admitted during the period of January 2009 - December 2011 were included. Wound swab was usually taken from the burnt areathat appears clinically deep, with pus or with thick eschar. In laboratory, microbial growth on any of the inoculatedplates gram staining, biochemical identification and antibiotic sensitivity by disc diffusion method was done. Statistical analysis was done through SPSS version 17.0 to determine the frequencies, mean ± SD and association of these cultures and sensitivity of isolated organisms with antibiotics.
Result:
Wound swab culture and sensitivity reports of 382 flame burnt patients were assessed. No significantrelation was found between age, gender and duration of stay of a patient and bacterial colonization. Cultureand sensitivity reports showing isolates of Staphylococcus aureus, Pseudomonas aeruginosa, Enterobacteraerogenes, Escherichia coli, Klebsiella pneumonia, Proteus as major microorganisms. Pseudomonas Aeroginosawas found to be the most commonly isolated microorganism 36.6%(f=140). It was found to be highly sensitivewith Imipenam throughout our period of study whereas Staphlococcus Aureus was found highly sensitivewith vancomycin, doxicillin and Amoxicillin+ clauvilaunic acid (in sequence with their percentage sensitivityin 2011).
Conclusion:
During the last three years, a change in susceptibility of antibiotic was found. Some of the antibioticswhich were 100 sensitive with common bacteria’s were posted to resistant during this period. Therefore, repeatedanalysis of microbes prevalent in Burn Care facility should be carried out in order to determine change in theirsensitivity spectrum to commonly prescribed antimicrobials to rule out wrong selection of empirical antibiotics. |
| first_indexed | 2024-04-13T15:48:22Z |
| format | Article |
| id | doaj.art-2927c68e6c13432882f647c5a70281db |
| institution | Directory Open Access Journal |
| issn | 1995-2198 2410-2180 |
| language | English |
| last_indexed | 2024-04-13T15:48:22Z |
| publishDate | 2013-04-01 |
| publisher | Dow University of Health Sciences |
| record_format | Article |
| series | Journal of the Dow University of Health Sciences |
| spelling | doaj.art-2927c68e6c13432882f647c5a70281db2022-12-22T02:40:54ZengDow University of Health SciencesJournal of the Dow University of Health Sciences1995-21982410-21802013-04-0172495371Pattern of Microorganisms Isolated from Flame Burn Wounds, and Their Trends of Susceptibility to Antibiotic During Past Three YearsEhmer Al-Ibran0Sidra Meraj1Mahwish Nasim2Maria Khan3Masood Hussain Rao4Burns Center, Civil Hospital & Dow University of Health Sciences, Karachi, Pakistan.Student, Fourth Year MBBS, Dow University of Health Sciences, Karachi, Pakistan.Student, Fourth Year MBBS, Dow University of Health Sciences, Karachi, PakistanStudent, Fourth Year MBBS, Dow University of Health Sciences, Karachi, Pakistan.Principal Research Officer, PMRC Research Center, Dow Medical College, Dow University of Health Sciences, Karachi, Pakistan.Objective: To determine the prevalent bacteria causing wound infection and their trend of susceptibility to antibiotics over a period of last three years in flame burn cases. This is an observational descriptive study conducted at the Burns Center, Civil Hospital Karachi. Methods: All available wound swabs culture and sensitivity reports of flame burn victims admitted during the period of January 2009 - December 2011 were included. Wound swab was usually taken from the burnt areathat appears clinically deep, with pus or with thick eschar. In laboratory, microbial growth on any of the inoculatedplates gram staining, biochemical identification and antibiotic sensitivity by disc diffusion method was done. Statistical analysis was done through SPSS version 17.0 to determine the frequencies, mean ± SD and association of these cultures and sensitivity of isolated organisms with antibiotics. Result: Wound swab culture and sensitivity reports of 382 flame burnt patients were assessed. No significantrelation was found between age, gender and duration of stay of a patient and bacterial colonization. Cultureand sensitivity reports showing isolates of Staphylococcus aureus, Pseudomonas aeruginosa, Enterobacteraerogenes, Escherichia coli, Klebsiella pneumonia, Proteus as major microorganisms. Pseudomonas Aeroginosawas found to be the most commonly isolated microorganism 36.6%(f=140). It was found to be highly sensitivewith Imipenam throughout our period of study whereas Staphlococcus Aureus was found highly sensitivewith vancomycin, doxicillin and Amoxicillin+ clauvilaunic acid (in sequence with their percentage sensitivityin 2011). Conclusion: During the last three years, a change in susceptibility of antibiotic was found. Some of the antibioticswhich were 100 sensitive with common bacteria’s were posted to resistant during this period. Therefore, repeatedanalysis of microbes prevalent in Burn Care facility should be carried out in order to determine change in theirsensitivity spectrum to commonly prescribed antimicrobials to rule out wrong selection of empirical antibiotics.http://www.jduhs.com/index.php/jduhs/article/view/71sensitivityantibioticspattern of antibiotic susceptibilitypattern of microorganisms. |
| spellingShingle | Ehmer Al-Ibran Sidra Meraj Mahwish Nasim Maria Khan Masood Hussain Rao Pattern of Microorganisms Isolated from Flame Burn Wounds, and Their Trends of Susceptibility to Antibiotic During Past Three Years Journal of the Dow University of Health Sciences sensitivity antibiotics pattern of antibiotic susceptibility pattern of microorganisms. |
| title | Pattern of Microorganisms Isolated from Flame Burn Wounds, and Their Trends of Susceptibility to Antibiotic During Past Three Years |
| title_full | Pattern of Microorganisms Isolated from Flame Burn Wounds, and Their Trends of Susceptibility to Antibiotic During Past Three Years |
| title_fullStr | Pattern of Microorganisms Isolated from Flame Burn Wounds, and Their Trends of Susceptibility to Antibiotic During Past Three Years |
| title_full_unstemmed | Pattern of Microorganisms Isolated from Flame Burn Wounds, and Their Trends of Susceptibility to Antibiotic During Past Three Years |
| title_short | Pattern of Microorganisms Isolated from Flame Burn Wounds, and Their Trends of Susceptibility to Antibiotic During Past Three Years |
| title_sort | pattern of microorganisms isolated from flame burn wounds and their trends of susceptibility to antibiotic during past three years |
| topic | sensitivity antibiotics pattern of antibiotic susceptibility pattern of microorganisms. |
| url | http://www.jduhs.com/index.php/jduhs/article/view/71 |
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