Impact of e-cigarette vaping aerosol exposure in pregnancy on mTOR signaling in rat fetal hippocampus
Electronic cigarette (e-cig) use during pregnancy has become a major health concern in recent years and many view them as less harmful and may help quit or reduce combustible cigarettes. Implementing a state-of-the-art engineered vaping system, comprising an atomizer similar to those sold in vape sh...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2023.1217127/full |
| _version_ | 1797793710712291328 |
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| author | Jehoon Lee Marcus R. Orzabal Vishal D. Naik Jayanth Ramadoss Jayanth Ramadoss |
| author_facet | Jehoon Lee Marcus R. Orzabal Vishal D. Naik Jayanth Ramadoss Jayanth Ramadoss |
| author_sort | Jehoon Lee |
| collection | DOAJ |
| description | Electronic cigarette (e-cig) use during pregnancy has become a major health concern in recent years and many view them as less harmful and may help quit or reduce combustible cigarettes. Implementing a state-of-the-art engineered vaping system, comprising an atomizer similar to those sold in vape shops, we aimed to utilize a translational e-cig inhalation delivery method to provide crucial information on the impact of prenatal e-cig aerosols on the developing brain hippocampal mTOR system in a rat model system. Gestational e-cig vaping significantly increased P-mTOR levels (p < 0.05) in the rat fetal hippocampi in the nicotine group (comprising of VG/PG + nicotine) compared to the control and the juice (comprising of VG/PG) groups. Total mTOR expression was not different among groups. Immunofluorescence imaging demonstrated P-mTOR was detected exclusively in the granule cells of the dentate gyrus of the fetal hippocampus. E-cig did not alter DEPTOR, but RAPTOR and RICTOR were higher (p < 0.05) in the Nicotine group. Gestational e-cig vaping with nicotine increased (p < 0.05) the activity and expression of 4EBP1, p70S6K, but decreased (p < 0.05) P-PKCα in the fetal hippocampi. In summary, dysregulation of mTORC1 and the related mTORC2, their activity, and downstream proteins together may play a critical role in e-cig-vaping-induced neurobiological phenotypes during development. |
| first_indexed | 2024-03-13T02:52:15Z |
| format | Article |
| id | doaj.art-29297e7b41a24820b351147cc3450d43 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-03-13T02:52:15Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-29297e7b41a24820b351147cc3450d432023-06-28T09:35:13ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2023-06-011710.3389/fnins.2023.12171271217127Impact of e-cigarette vaping aerosol exposure in pregnancy on mTOR signaling in rat fetal hippocampusJehoon Lee0Marcus R. Orzabal1Vishal D. Naik2Jayanth Ramadoss3Jayanth Ramadoss4Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX, United StatesDepartment of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX, United StatesDepartment of Obstetrics and Gynecology, C.S. Mott Center for Human Growth and Development, School of Medicine, Wayne State University, Detroit, MI, United StatesDepartment of Obstetrics and Gynecology, C.S. Mott Center for Human Growth and Development, School of Medicine, Wayne State University, Detroit, MI, United StatesDepartment of Physiology, School of Medicine, Wayne State University, Detroit, MI, United StatesElectronic cigarette (e-cig) use during pregnancy has become a major health concern in recent years and many view them as less harmful and may help quit or reduce combustible cigarettes. Implementing a state-of-the-art engineered vaping system, comprising an atomizer similar to those sold in vape shops, we aimed to utilize a translational e-cig inhalation delivery method to provide crucial information on the impact of prenatal e-cig aerosols on the developing brain hippocampal mTOR system in a rat model system. Gestational e-cig vaping significantly increased P-mTOR levels (p < 0.05) in the rat fetal hippocampi in the nicotine group (comprising of VG/PG + nicotine) compared to the control and the juice (comprising of VG/PG) groups. Total mTOR expression was not different among groups. Immunofluorescence imaging demonstrated P-mTOR was detected exclusively in the granule cells of the dentate gyrus of the fetal hippocampus. E-cig did not alter DEPTOR, but RAPTOR and RICTOR were higher (p < 0.05) in the Nicotine group. Gestational e-cig vaping with nicotine increased (p < 0.05) the activity and expression of 4EBP1, p70S6K, but decreased (p < 0.05) P-PKCα in the fetal hippocampi. In summary, dysregulation of mTORC1 and the related mTORC2, their activity, and downstream proteins together may play a critical role in e-cig-vaping-induced neurobiological phenotypes during development.https://www.frontiersin.org/articles/10.3389/fnins.2023.1217127/fullpregnancydevelopmentfetalnicotinebrainvaping |
| spellingShingle | Jehoon Lee Marcus R. Orzabal Vishal D. Naik Jayanth Ramadoss Jayanth Ramadoss Impact of e-cigarette vaping aerosol exposure in pregnancy on mTOR signaling in rat fetal hippocampus Frontiers in Neuroscience pregnancy development fetal nicotine brain vaping |
| title | Impact of e-cigarette vaping aerosol exposure in pregnancy on mTOR signaling in rat fetal hippocampus |
| title_full | Impact of e-cigarette vaping aerosol exposure in pregnancy on mTOR signaling in rat fetal hippocampus |
| title_fullStr | Impact of e-cigarette vaping aerosol exposure in pregnancy on mTOR signaling in rat fetal hippocampus |
| title_full_unstemmed | Impact of e-cigarette vaping aerosol exposure in pregnancy on mTOR signaling in rat fetal hippocampus |
| title_short | Impact of e-cigarette vaping aerosol exposure in pregnancy on mTOR signaling in rat fetal hippocampus |
| title_sort | impact of e cigarette vaping aerosol exposure in pregnancy on mtor signaling in rat fetal hippocampus |
| topic | pregnancy development fetal nicotine brain vaping |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2023.1217127/full |
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