A novel pattern recognition protein of the Chinese oak silkmoth, Antheraea pernyi, is involved in the pro-PO activating system
In this paper, we firstly reported a C-type lectin cDNA clone of1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR andRACE techniques. The full-length cDNA contains an openreading frame encoding 308 amino acid residues which hastwo different carbohydrate-recognition domains (CRDs) arrangedin t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Korean Society for Biochemistry and Molecular Biology
2013-07-01
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| Series: | BMB Reports |
| Subjects: | |
| Online Access: | http://bmbreports.org/jbmb/pdf.php?data=MTMxMDAyMTZAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni03JTVEMTMwNzI3MTkzMl8lMjgzNTgtMzYzJTI5Qk1CXzEzLTAwOS5wZGY= |
| Summary: | In this paper, we firstly reported a C-type lectin cDNA clone of1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR andRACE techniques. The full-length cDNA contains an openreading frame encoding 308 amino acid residues which hastwo different carbohydrate-recognition domains (CRDs) arrangedin tandem. To investigate the biological activities in theinnate immunity, recombinant Ap-CTL was expressed in E. coliwith a 6-histidine at the amino-terminus (Ap-rCTL). Besidesacted as a broad-spectrum recognition protein binding to awide range of PAMPs and microorganisms, Ap-rCTL also hadthe ability to recognize and trigger the agglutination of bacteriaand fungi. In the proPO activation assay, Ap-rCTL specificallyrestored the PO activity of hemolymph blocked by anti-Ap-rCTL antibody in the presence of different PAMPs ormicroorganisms. In summary, Ap-rCTL plays an important rolein insect innate immunity as an pattern recognition protein.[BMB Reports 2013; 46(7): 358-363] |
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| ISSN: | 1976-6696 1976-670X |