Mapping Quantitative Trait Loci for Lettuce Resistance to Verticillium dahliae Race 3, Plant Development, and Leaf Color Using an Ultra-High-Density Bin Map Constructed from F2 Progeny
Verticillium wilt is one of the most devastating soilborne diseases in lettuce, and the use of host resistance is the most optimal choice for its management. This study focused on identifying and mapping the genetic loci for resistance against Verticillium dahliae race 3 in a mapping population of 2...
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The American Phytopathological Society
2022-09-01
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| Series: | PhytoFrontiers |
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| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-11-21-0078-R |
| _version_ | 1827095654301696000 |
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| author | Ivan Simko Krishna D. Puri Nikhilesh Dhar Hui Peng Krishna V. Subbarao |
| author_facet | Ivan Simko Krishna D. Puri Nikhilesh Dhar Hui Peng Krishna V. Subbarao |
| author_sort | Ivan Simko |
| collection | DOAJ |
| description | Verticillium wilt is one of the most devastating soilborne diseases in lettuce, and the use of host resistance is the most optimal choice for its management. This study focused on identifying and mapping the genetic loci for resistance against Verticillium dahliae race 3 in a mapping population of 200 F2:3 families developed from a cross between moderately resistant red-leaf lettuce ‘Sentry’ and susceptible green-leaf lettuce ‘La Brillante’. The population was genotyped using the tunable genotyping-by-sequencing (tGBS) approach. An ultra-high-density genetic linkage map containing 34,838 single nucleotide polymorphism markers grouped into 1,734 bins was constructed using F2 progeny and a sliding window approach. Three quantitative trait loci (QTLs) for resistance to V. dahliae race 3 were located on linkage groups (LGs) LG 2 (qVR3-2.1) and LG 4 (qVR3-4.1 and qVR3-4.2). Each of these QTLs explained up to ∼10% of the total phenotypic variation for the trait. At each locus, the resistance alleles were derived from cultivar Sentry that is partially resistant to the pathogen. Additional loci resistant to the disease are expected in this population, and transgressive segregation indicates that some of those loci could originate from the susceptible cultivar La Brillante. In addition, two QTLs for plant development were identified on LG 2 (qIPD-2.1) and LG 7 (qIPD-7.1), although no relationship was detected between resistance in these genotypes and the rate of plant growth. A major effect of QTL for red leaf color was detected on LG 9 (qRLC-9.1). Candidate genes linked to some of the QTLs for V. dahliae race 3 resistance, plant development, and leaf color were identified. The QTLs for resistance identified in Sentry could diversify the resistance gene pool and provide an alternative tool to manage a newly emerged V. dahliae race 3. [Figure: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2690-5442 |
| language | English |
| last_indexed | 2025-03-20T07:03:19Z |
| publishDate | 2022-09-01 |
| publisher | The American Phytopathological Society |
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| spelling | doaj.art-292ce6e65c894143b7cc5a39d5162efe2024-10-01T12:35:42ZengThe American Phytopathological SocietyPhytoFrontiers2690-54422022-09-012325726710.1094/PHYTOFR-11-21-0078-RMapping Quantitative Trait Loci for Lettuce Resistance to Verticillium dahliae Race 3, Plant Development, and Leaf Color Using an Ultra-High-Density Bin Map Constructed from F2 ProgenyIvan Simko0Krishna D. Puri1Nikhilesh Dhar2Hui Peng3Krishna V. Subbarao4United States Department of Agriculture, Agricultural Research Service, Crop Improvement and Protection Research Unit, Salinas, CA 93905Department of Plant Pathology, University of California, Davis, c/o U.S. Agricultural Research Station, Salinas, CA 93905Department of Plant Pathology, University of California, Davis, c/o U.S. Agricultural Research Station, Salinas, CA 93905Department of Plant Pathology, University of California, Davis, c/o U.S. Agricultural Research Station, Salinas, CA 93905Department of Plant Pathology, University of California, Davis, c/o U.S. Agricultural Research Station, Salinas, CA 93905Verticillium wilt is one of the most devastating soilborne diseases in lettuce, and the use of host resistance is the most optimal choice for its management. This study focused on identifying and mapping the genetic loci for resistance against Verticillium dahliae race 3 in a mapping population of 200 F2:3 families developed from a cross between moderately resistant red-leaf lettuce ‘Sentry’ and susceptible green-leaf lettuce ‘La Brillante’. The population was genotyped using the tunable genotyping-by-sequencing (tGBS) approach. An ultra-high-density genetic linkage map containing 34,838 single nucleotide polymorphism markers grouped into 1,734 bins was constructed using F2 progeny and a sliding window approach. Three quantitative trait loci (QTLs) for resistance to V. dahliae race 3 were located on linkage groups (LGs) LG 2 (qVR3-2.1) and LG 4 (qVR3-4.1 and qVR3-4.2). Each of these QTLs explained up to ∼10% of the total phenotypic variation for the trait. At each locus, the resistance alleles were derived from cultivar Sentry that is partially resistant to the pathogen. Additional loci resistant to the disease are expected in this population, and transgressive segregation indicates that some of those loci could originate from the susceptible cultivar La Brillante. In addition, two QTLs for plant development were identified on LG 2 (qIPD-2.1) and LG 7 (qIPD-7.1), although no relationship was detected between resistance in these genotypes and the rate of plant growth. A major effect of QTL for red leaf color was detected on LG 9 (qRLC-9.1). Candidate genes linked to some of the QTLs for V. dahliae race 3 resistance, plant development, and leaf color were identified. The QTLs for resistance identified in Sentry could diversify the resistance gene pool and provide an alternative tool to manage a newly emerged V. dahliae race 3. [Figure: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-11-21-0078-Rbreedingdisease resistancegrowth and developmentinheritanceleaf colorlettuce |
| spellingShingle | Ivan Simko Krishna D. Puri Nikhilesh Dhar Hui Peng Krishna V. Subbarao Mapping Quantitative Trait Loci for Lettuce Resistance to Verticillium dahliae Race 3, Plant Development, and Leaf Color Using an Ultra-High-Density Bin Map Constructed from F2 Progeny PhytoFrontiers breeding disease resistance growth and development inheritance leaf color lettuce |
| title | Mapping Quantitative Trait Loci for Lettuce Resistance to Verticillium dahliae Race 3, Plant Development, and Leaf Color Using an Ultra-High-Density Bin Map Constructed from F2 Progeny |
| title_full | Mapping Quantitative Trait Loci for Lettuce Resistance to Verticillium dahliae Race 3, Plant Development, and Leaf Color Using an Ultra-High-Density Bin Map Constructed from F2 Progeny |
| title_fullStr | Mapping Quantitative Trait Loci for Lettuce Resistance to Verticillium dahliae Race 3, Plant Development, and Leaf Color Using an Ultra-High-Density Bin Map Constructed from F2 Progeny |
| title_full_unstemmed | Mapping Quantitative Trait Loci for Lettuce Resistance to Verticillium dahliae Race 3, Plant Development, and Leaf Color Using an Ultra-High-Density Bin Map Constructed from F2 Progeny |
| title_short | Mapping Quantitative Trait Loci for Lettuce Resistance to Verticillium dahliae Race 3, Plant Development, and Leaf Color Using an Ultra-High-Density Bin Map Constructed from F2 Progeny |
| title_sort | mapping quantitative trait loci for lettuce resistance to verticillium dahliae race 3 plant development and leaf color using an ultra high density bin map constructed from f2 progeny |
| topic | breeding disease resistance growth and development inheritance leaf color lettuce |
| url | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-11-21-0078-R |
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