Indian Pangolin (Manis crassicaudata), an endangered mammalian species under IUCN red list. In India, small population of Chines Pangolin (Manis pentadactyla) are also present in the eastern part and protected under the Wildlife (Protection) Act, 1972 as a schedule I species along with Indian Pangol...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2016-12-01
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| Series: | Egyptian Journal of Forensic Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2090536X16300454 |
| _version_ | 1818948615122452480 |
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| author | Ved Prakash Kumar Ankita Rajpoot Mukesh Malay Shukla Dhyanendra Kumar Surendra Prakash Goyal |
| author_facet | Ved Prakash Kumar Ankita Rajpoot Mukesh Malay Shukla Dhyanendra Kumar Surendra Prakash Goyal |
| author_sort | Ved Prakash Kumar |
| collection | DOAJ |
| description | Indian Pangolin (Manis crassicaudata), an endangered mammalian species under IUCN red list. In India, small population of Chines Pangolin (Manis pentadactyla) are also present in the eastern part and protected under the Wildlife (Protection) Act, 1972 as a schedule I species along with Indian Pangolin. In national and international market the Indian Pangolin are highly in trade principally for its meat and scales. In curbing illegal wildlife, trade of Indian Pangolin on national and international level wildlife DNA forensics plays an important role in the identification of species from the seized material. We received n = 15 wildlife seizures assumed to be the scales of Pangolin from different state forest and the custom department for molecular analysis. The seizures sequenced for two mitochondrial genes i.e. Cyt b and 16S rRNA, which are often used in wildlife DNA forensic to species identification. The sequences thus generated from these seizures compared to the NCBI database through blast search tool and our laboratory-generated references, yielding 100% similarity with Indian Pangolin. We identified twelve (n = 12) Cyt b and five (n = 5) 16S rRNA species specific SNPs, fixed in Indian Pangolin. This study shows, the importance of authenticated references, DNA sequence data availability, highlighting the application of DNA forensics in identifying species from scales. |
| first_indexed | 2024-12-20T08:49:37Z |
| format | Article |
| id | doaj.art-292e8ab6394f4775a5dce348f9576cfa |
| institution | Directory Open Access Journal |
| issn | 2090-536X |
| language | English |
| last_indexed | 2024-12-20T08:49:37Z |
| publishDate | 2016-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Forensic Sciences |
| spelling | doaj.art-292e8ab6394f4775a5dce348f9576cfa2022-12-21T19:46:09ZengSpringerOpenEgyptian Journal of Forensic Sciences2090-536X2016-12-016452453310.1016/j.ejfs.2016.06.008Ved Prakash Kumar0Ankita Rajpoot1Mukesh2Malay Shukla3Dhyanendra Kumar4Surendra Prakash Goyal5Wildlife Institute of India, Post Box # 18, Chandrabani, Dehradun 248001, Uttarakhand, IndiaZoological Survey of India, NRC, Kaulagarh, Dehradun 248001, Uttarakhand, IndiaAmity Institute of Wildlife Sciences, Amity University, Sector 125, Noida 201 313, Uttar Pradesh, IndiaGujrat Forensic University, Gandhinagar 382007, Gujrat, IndiaVeer Kunwar Singh University, Arrah 802301, Bihar, IndiaWildlife Institute of India, Post Box # 18, Chandrabani, Dehradun 248001, Uttarakhand, IndiaIndian Pangolin (Manis crassicaudata), an endangered mammalian species under IUCN red list. In India, small population of Chines Pangolin (Manis pentadactyla) are also present in the eastern part and protected under the Wildlife (Protection) Act, 1972 as a schedule I species along with Indian Pangolin. In national and international market the Indian Pangolin are highly in trade principally for its meat and scales. In curbing illegal wildlife, trade of Indian Pangolin on national and international level wildlife DNA forensics plays an important role in the identification of species from the seized material. We received n = 15 wildlife seizures assumed to be the scales of Pangolin from different state forest and the custom department for molecular analysis. The seizures sequenced for two mitochondrial genes i.e. Cyt b and 16S rRNA, which are often used in wildlife DNA forensic to species identification. The sequences thus generated from these seizures compared to the NCBI database through blast search tool and our laboratory-generated references, yielding 100% similarity with Indian Pangolin. We identified twelve (n = 12) Cyt b and five (n = 5) 16S rRNA species specific SNPs, fixed in Indian Pangolin. This study shows, the importance of authenticated references, DNA sequence data availability, highlighting the application of DNA forensics in identifying species from scales.http://www.sciencedirect.com/science/article/pii/S2090536X16300454DNA forensic scienceManis crassicaudataSpecies identificationIllegal trades and mitochondrial gene |
| spellingShingle | Ved Prakash Kumar Ankita Rajpoot Mukesh Malay Shukla Dhyanendra Kumar Surendra Prakash Goyal Egyptian Journal of Forensic Sciences DNA forensic science Manis crassicaudata Species identification Illegal trades and mitochondrial gene |
| topic | DNA forensic science Manis crassicaudata Species identification Illegal trades and mitochondrial gene |
| url | http://www.sciencedirect.com/science/article/pii/S2090536X16300454 |