Combination of the Endogenous lhcsr1 Promoter and Codon Usage Optimization Boosts Protein Expression in the Moss Physcomitrella patens

Combination of the Endogenous lhcsr1 Promoter and Codon Usage Optimization Boosts Protein Expression in the Moss Physcomitrella patens

The moss Physcomitrella patens is used both as an evo-devo model and biotechnological production system for metabolites and pharmaceuticals. Strong in vivo expression of genes of interest is important for production of recombinant proteins, e.g., selectable markers, fluorescent proteins, or enzymes....

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Bibliographic Details
Main Authors: Manuel Hiss, Lucas Schneider, Christopher Grosche, Melanie A. Barth, Christina Neu, Aikaterini Symeonidi, Kristian K. Ullrich, Pierre-François Perroud, Mareike Schallenberg-Rüdinger, Stefan A. Rensing
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-10-01
Series:Frontiers in Plant Science
Subjects:
Physcomitrella patens
codon usage
chlorophyll a/b binding protein
promoter
codon bias
green fluorescent protein (GFP)
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2017.01842/full

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