Effect of salicylic acid and methyl jasmonate in the production of phenolic compounds in plant cell suspension cultures of Thevetia peruviana
The objective was to enhance the production of the phenolic compounds in plant cell suspension cultures of T. peruviana at shake flask scale. The effects of salicylic acid (SA), methyl-jasmonate (MeJA) and the combination of both (SA/MeJA) were studied. Elicitor concentration, elicitation time and h...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-09-01
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| Series: | Biotechnology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X18300481 |
| _version_ | 1811303360985300992 |
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| author | Dary Mendoza Olmedo Cuaspud Juan Pablo Arias Orlando Ruiz Mario Arias |
| author_facet | Dary Mendoza Olmedo Cuaspud Juan Pablo Arias Orlando Ruiz Mario Arias |
| author_sort | Dary Mendoza |
| collection | DOAJ |
| description | The objective was to enhance the production of the phenolic compounds in plant cell suspension cultures of T. peruviana at shake flask scale. The effects of salicylic acid (SA), methyl-jasmonate (MeJA) and the combination of both (SA/MeJA) were studied. Elicitor concentration, elicitation time and harvest time of cells were optimized. Phenolic compound content (PCC), flavonoid content (FC) and antioxidant activity (AA) were determined by the folin-ciocalteu method, flavonoid-aluminum complexation method and the ABTS assay, respectively. Differences between intracellular metabolite profiles due to the mentioned treatments were analyzed by Thin-layer chromatography and High-performance liquid chromatography. Highest PCC, FC and AA were obtained under the following treatments: 3 μM MeJA > 3 μM MeJA/300 μM SA > 300 μM SA > control, when elicited on the 4th day and harvested 96-h post-elicitation. It was demonstrated that exposure to 3 μM MeJA increase 1.49-fold of PCC, 1.66-fold of AA and 2.55-fold of FC compared to the control culture. Keywords: Thevetia peruviana, Plant cell culture, Phenolic compounds, Methyl jasmonate, Salicylic acid |
| first_indexed | 2024-04-13T07:45:33Z |
| format | Article |
| id | doaj.art-292f5cb543a949cc9cafb28e8388f881 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-13T07:45:33Z |
| publishDate | 2018-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-292f5cb543a949cc9cafb28e8388f8812022-12-22T02:55:41ZengElsevierBiotechnology Reports2215-017X2018-09-0119Effect of salicylic acid and methyl jasmonate in the production of phenolic compounds in plant cell suspension cultures of Thevetia peruvianaDary Mendoza0Olmedo Cuaspud1Juan Pablo Arias2Orlando Ruiz3Mario Arias4Grupo de Investigación en Biotecnología Industrial, Laboratorio de Bioconversiones, Universidad Nacional de Colombia, Calle 59A No.63-20 Bloque 19A-313, Medellín, Colombia; Grupo de Productos Naturales y Bioquímica de Macromoléculas, Facultad de Ciencias, Universidad del Atlántico, Km 7 via a Puerto Colombia, Colombia; Corresponding author at: Laboratorio de Bioconversiones, Universidad Nacional de Colombia, Calle 59A No.63-20 Bloque 19A-313, Medellín, Colombia.Grupo de Investigación en Biotecnología Industrial, Laboratorio de Bioconversiones, Universidad Nacional de Colombia, Calle 59A No.63-20 Bloque 19A-313, Medellín, ColombiaGrupo de Investigación en Biotecnología Industrial, Laboratorio de Bioconversiones, Universidad Nacional de Colombia, Calle 59A No.63-20 Bloque 19A-313, Medellín, ColombiaLaboratorio de Suelos, Universidad Nacional de Colombia, Calle 59A No.63-20 Bloque 14-203, Medellín, ColombiaGrupo de Investigación en Biotecnología Industrial, Laboratorio de Bioconversiones, Universidad Nacional de Colombia, Calle 59A No.63-20 Bloque 19A-313, Medellín, ColombiaThe objective was to enhance the production of the phenolic compounds in plant cell suspension cultures of T. peruviana at shake flask scale. The effects of salicylic acid (SA), methyl-jasmonate (MeJA) and the combination of both (SA/MeJA) were studied. Elicitor concentration, elicitation time and harvest time of cells were optimized. Phenolic compound content (PCC), flavonoid content (FC) and antioxidant activity (AA) were determined by the folin-ciocalteu method, flavonoid-aluminum complexation method and the ABTS assay, respectively. Differences between intracellular metabolite profiles due to the mentioned treatments were analyzed by Thin-layer chromatography and High-performance liquid chromatography. Highest PCC, FC and AA were obtained under the following treatments: 3 μM MeJA > 3 μM MeJA/300 μM SA > 300 μM SA > control, when elicited on the 4th day and harvested 96-h post-elicitation. It was demonstrated that exposure to 3 μM MeJA increase 1.49-fold of PCC, 1.66-fold of AA and 2.55-fold of FC compared to the control culture. Keywords: Thevetia peruviana, Plant cell culture, Phenolic compounds, Methyl jasmonate, Salicylic acidhttp://www.sciencedirect.com/science/article/pii/S2215017X18300481 |
| spellingShingle | Dary Mendoza Olmedo Cuaspud Juan Pablo Arias Orlando Ruiz Mario Arias Effect of salicylic acid and methyl jasmonate in the production of phenolic compounds in plant cell suspension cultures of Thevetia peruviana Biotechnology Reports |
| title | Effect of salicylic acid and methyl jasmonate in the production of phenolic compounds in plant cell suspension cultures of Thevetia peruviana |
| title_full | Effect of salicylic acid and methyl jasmonate in the production of phenolic compounds in plant cell suspension cultures of Thevetia peruviana |
| title_fullStr | Effect of salicylic acid and methyl jasmonate in the production of phenolic compounds in plant cell suspension cultures of Thevetia peruviana |
| title_full_unstemmed | Effect of salicylic acid and methyl jasmonate in the production of phenolic compounds in plant cell suspension cultures of Thevetia peruviana |
| title_short | Effect of salicylic acid and methyl jasmonate in the production of phenolic compounds in plant cell suspension cultures of Thevetia peruviana |
| title_sort | effect of salicylic acid and methyl jasmonate in the production of phenolic compounds in plant cell suspension cultures of thevetia peruviana |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X18300481 |
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