Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy
Standard benchmarking of single-molecule localization microscopy cannot quantify nanoscale accuracy of arbitrary datasets. Here, the authors present Wasserstein-induced flux, a method using a chosen perturbation and knowledge of the imaging system to measure confidence of individual localizations.
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-20056-9 |
| _version_ | 1818435847969570816 |
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| author | Hesam Mazidi Tianben Ding Arye Nehorai Matthew D. Lew |
| author_facet | Hesam Mazidi Tianben Ding Arye Nehorai Matthew D. Lew |
| author_sort | Hesam Mazidi |
| collection | DOAJ |
| description | Standard benchmarking of single-molecule localization microscopy cannot quantify nanoscale accuracy of arbitrary datasets. Here, the authors present Wasserstein-induced flux, a method using a chosen perturbation and knowledge of the imaging system to measure confidence of individual localizations. |
| first_indexed | 2024-12-14T16:59:24Z |
| format | Article |
| id | doaj.art-293de6b5849b489dbc388634cd7aeec1 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-14T16:59:24Z |
| publishDate | 2020-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-293de6b5849b489dbc388634cd7aeec12022-12-21T22:53:53ZengNature PortfolioNature Communications2041-17232020-12-0111111110.1038/s41467-020-20056-9Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopyHesam Mazidi0Tianben Ding1Arye Nehorai2Matthew D. Lew3Department of Electrical and Systems Engineering, Washington University in St. LouisDepartment of Electrical and Systems Engineering, Washington University in St. LouisDepartment of Electrical and Systems Engineering, Washington University in St. LouisDepartment of Electrical and Systems Engineering, Washington University in St. LouisStandard benchmarking of single-molecule localization microscopy cannot quantify nanoscale accuracy of arbitrary datasets. Here, the authors present Wasserstein-induced flux, a method using a chosen perturbation and knowledge of the imaging system to measure confidence of individual localizations.https://doi.org/10.1038/s41467-020-20056-9 |
| spellingShingle | Hesam Mazidi Tianben Ding Arye Nehorai Matthew D. Lew Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy Nature Communications |
| title | Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy |
| title_full | Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy |
| title_fullStr | Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy |
| title_full_unstemmed | Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy |
| title_short | Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy |
| title_sort | quantifying accuracy and heterogeneity in single molecule super resolution microscopy |
| url | https://doi.org/10.1038/s41467-020-20056-9 |
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