Evolution and Expression Patterns of CYC/TB1 Genes in Anacyclus: Phylogenetic Insights for Floral Symmetry Genes in Asteraceae
Homologs of the CYC/TB1 gene family have been independently recruited many times across the eudicots to control aspects of floral symmetry The family Asteraceae exhibits the largest known diversification in this gene paralog family accompanied by a parallel morphological floral richness in its speci...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00589/full |
| _version_ | 1819105821983768576 |
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| author | Javier Fuertes-Aguilar María A. Bello Pilar Cubas Inés Álvarez Guillermo Sanjuanbenito |
| author_facet | Javier Fuertes-Aguilar María A. Bello Pilar Cubas Inés Álvarez Guillermo Sanjuanbenito |
| author_sort | Javier Fuertes-Aguilar |
| collection | DOAJ |
| description | Homologs of the CYC/TB1 gene family have been independently recruited many times across the eudicots to control aspects of floral symmetry The family Asteraceae exhibits the largest known diversification in this gene paralog family accompanied by a parallel morphological floral richness in its specialized head-like inflorescence. In Asteraceae, whether or not CYC/TB1 gene floral symmetry function is preserved along organismic and gene lineages is unknown. In this study, we used phylogenetic, structural and expression analyses focused on the highly derived genus Anacyclus (tribe Anthemidae) to address this question. Phylogenetic reconstruction recovered eight main gene lineages present in Asteraceae: two from CYC1, four from CYC2 and two from CYC3-like genes. The species phylogeny was recovered in most of the gene lineages, allowing the delimitation of orthologous sets of CYC/TB1 genes in Asteraceae. Quantitative real-time PCR analysis indicated that in Anacyclus three of the four isolated CYC2 genes are more highly expressed in ray flowers. The expression of the four AcCYC2 genes overlaps in several organs including the ligule of ray flowers, as well as in anthers and ovules throughout development. |
| first_indexed | 2024-12-22T02:28:21Z |
| format | Article |
| id | doaj.art-293e69567502441b900f651d93203b37 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T02:28:21Z |
| publishDate | 2017-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-293e69567502441b900f651d93203b372022-12-21T18:41:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-04-01810.3389/fpls.2017.00589236218Evolution and Expression Patterns of CYC/TB1 Genes in Anacyclus: Phylogenetic Insights for Floral Symmetry Genes in AsteraceaeJavier Fuertes-Aguilar0María A. Bello1Pilar Cubas2Inés Álvarez3Guillermo Sanjuanbenito4Plant Evolutionary Biology Group, Real Jardín Botánico (CSIC)Madrid, SpainPlant Evolutionary Biology Group, Real Jardín Botánico (CSIC)Madrid, SpainDepartment of Plant Molecular Genetics, Centro Nacional de Biotecnología, CSIC-Universidad Autónoma de MadridMadrid, SpainPlant Evolutionary Biology Group, Real Jardín Botánico (CSIC)Madrid, SpainPlant Evolutionary Biology Group, Real Jardín Botánico (CSIC)Madrid, SpainHomologs of the CYC/TB1 gene family have been independently recruited many times across the eudicots to control aspects of floral symmetry The family Asteraceae exhibits the largest known diversification in this gene paralog family accompanied by a parallel morphological floral richness in its specialized head-like inflorescence. In Asteraceae, whether or not CYC/TB1 gene floral symmetry function is preserved along organismic and gene lineages is unknown. In this study, we used phylogenetic, structural and expression analyses focused on the highly derived genus Anacyclus (tribe Anthemidae) to address this question. Phylogenetic reconstruction recovered eight main gene lineages present in Asteraceae: two from CYC1, four from CYC2 and two from CYC3-like genes. The species phylogeny was recovered in most of the gene lineages, allowing the delimitation of orthologous sets of CYC/TB1 genes in Asteraceae. Quantitative real-time PCR analysis indicated that in Anacyclus three of the four isolated CYC2 genes are more highly expressed in ray flowers. The expression of the four AcCYC2 genes overlaps in several organs including the ligule of ray flowers, as well as in anthers and ovules throughout development.http://journal.frontiersin.org/article/10.3389/fpls.2017.00589/fullAnacyclusAsteraceaeCYC2 diversificationCYCLOIDEACYC/TB1floral symmetry |
| spellingShingle | Javier Fuertes-Aguilar María A. Bello Pilar Cubas Inés Álvarez Guillermo Sanjuanbenito Evolution and Expression Patterns of CYC/TB1 Genes in Anacyclus: Phylogenetic Insights for Floral Symmetry Genes in Asteraceae Frontiers in Plant Science Anacyclus Asteraceae CYC2 diversification CYCLOIDEA CYC/TB1 floral symmetry |
| title | Evolution and Expression Patterns of CYC/TB1 Genes in Anacyclus: Phylogenetic Insights for Floral Symmetry Genes in Asteraceae |
| title_full | Evolution and Expression Patterns of CYC/TB1 Genes in Anacyclus: Phylogenetic Insights for Floral Symmetry Genes in Asteraceae |
| title_fullStr | Evolution and Expression Patterns of CYC/TB1 Genes in Anacyclus: Phylogenetic Insights for Floral Symmetry Genes in Asteraceae |
| title_full_unstemmed | Evolution and Expression Patterns of CYC/TB1 Genes in Anacyclus: Phylogenetic Insights for Floral Symmetry Genes in Asteraceae |
| title_short | Evolution and Expression Patterns of CYC/TB1 Genes in Anacyclus: Phylogenetic Insights for Floral Symmetry Genes in Asteraceae |
| title_sort | evolution and expression patterns of cyc tb1 genes in anacyclus phylogenetic insights for floral symmetry genes in asteraceae |
| topic | Anacyclus Asteraceae CYC2 diversification CYCLOIDEA CYC/TB1 floral symmetry |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00589/full |
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