Oxygen-carbon nanotubes as a chemotherapy sensitizer for paclitaxel in breast cancer treatment.
To study the in vivo and in vitro effects of adding oxygen carbon nanotubes (CNTs) to chemotherapy for breast cancer.MCF-7 and SK-BR-3 breast cancer cells were co-cultured with paclitaxel and then exposed to oxygen-CNTs under hypoxic conditions. Cell proliferation, viability, and apoptosis rate were...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4121325?pdf=render |
| _version_ | 1818047779383017472 |
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| author | Yongkun Wang Chuanying Wang Yijun Jia Xianhua Cheng Qing Lin Mingjie Zhu Yunshu Lu Longlong Ding Ziyi Weng Kejin Wu |
| author_facet | Yongkun Wang Chuanying Wang Yijun Jia Xianhua Cheng Qing Lin Mingjie Zhu Yunshu Lu Longlong Ding Ziyi Weng Kejin Wu |
| author_sort | Yongkun Wang |
| collection | DOAJ |
| description | To study the in vivo and in vitro effects of adding oxygen carbon nanotubes (CNTs) to chemotherapy for breast cancer.MCF-7 and SK-BR-3 breast cancer cells were co-cultured with paclitaxel and then exposed to oxygen-CNTs under hypoxic conditions. Cell proliferation, viability, and apoptosis rate were analyzed. Hypoxia-inducible factor-1 alpha (HIF-1α) expression was measured using reverse transcription-polymerase chain reaction (RT-PCR) and western blot. Nude mice were used as a human breast cancer model to explore the impact of oxygen-CNTs on the in vivo chemotherapeutic effect of paclitaxel.Oxygen-CNTs had no significant effects on the growth of breast cancer cells under normoxia and hypoxia. However, in the hypoxic environment, oxygen-CNTs significantly enhanced the inhibitory effect of paclitaxel on cell proliferation, as well as the apoptosis rate. Under hypoxia, downregulation of HIF-1α and upregulation of caspase-3, caspase-8, caspase-9, LC3 and Beclin-1 were observed when paclitaxel was combined with oxygen-CNT. Furthermore, addition of oxygen-CNTs to chemotherapy was found to significantly reduce tumor weight in the tumor-bearing mice model.Oxygen-CNTs can significantly increase the chemotherapeutic effect of paclitaxel on breast cancer cells. Oxygen-CNTs may be a potential chemosensitizer in breast cancer therapy. |
| first_indexed | 2024-12-10T10:11:13Z |
| format | Article |
| id | doaj.art-293ea5c127dd415eb77206396f5aada5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T10:11:13Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-293ea5c127dd415eb77206396f5aada52022-12-22T01:53:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10420910.1371/journal.pone.0104209Oxygen-carbon nanotubes as a chemotherapy sensitizer for paclitaxel in breast cancer treatment.Yongkun WangChuanying WangYijun JiaXianhua ChengQing LinMingjie ZhuYunshu LuLonglong DingZiyi WengKejin WuTo study the in vivo and in vitro effects of adding oxygen carbon nanotubes (CNTs) to chemotherapy for breast cancer.MCF-7 and SK-BR-3 breast cancer cells were co-cultured with paclitaxel and then exposed to oxygen-CNTs under hypoxic conditions. Cell proliferation, viability, and apoptosis rate were analyzed. Hypoxia-inducible factor-1 alpha (HIF-1α) expression was measured using reverse transcription-polymerase chain reaction (RT-PCR) and western blot. Nude mice were used as a human breast cancer model to explore the impact of oxygen-CNTs on the in vivo chemotherapeutic effect of paclitaxel.Oxygen-CNTs had no significant effects on the growth of breast cancer cells under normoxia and hypoxia. However, in the hypoxic environment, oxygen-CNTs significantly enhanced the inhibitory effect of paclitaxel on cell proliferation, as well as the apoptosis rate. Under hypoxia, downregulation of HIF-1α and upregulation of caspase-3, caspase-8, caspase-9, LC3 and Beclin-1 were observed when paclitaxel was combined with oxygen-CNT. Furthermore, addition of oxygen-CNTs to chemotherapy was found to significantly reduce tumor weight in the tumor-bearing mice model.Oxygen-CNTs can significantly increase the chemotherapeutic effect of paclitaxel on breast cancer cells. Oxygen-CNTs may be a potential chemosensitizer in breast cancer therapy.http://europepmc.org/articles/PMC4121325?pdf=render |
| spellingShingle | Yongkun Wang Chuanying Wang Yijun Jia Xianhua Cheng Qing Lin Mingjie Zhu Yunshu Lu Longlong Ding Ziyi Weng Kejin Wu Oxygen-carbon nanotubes as a chemotherapy sensitizer for paclitaxel in breast cancer treatment. PLoS ONE |
| title | Oxygen-carbon nanotubes as a chemotherapy sensitizer for paclitaxel in breast cancer treatment. |
| title_full | Oxygen-carbon nanotubes as a chemotherapy sensitizer for paclitaxel in breast cancer treatment. |
| title_fullStr | Oxygen-carbon nanotubes as a chemotherapy sensitizer for paclitaxel in breast cancer treatment. |
| title_full_unstemmed | Oxygen-carbon nanotubes as a chemotherapy sensitizer for paclitaxel in breast cancer treatment. |
| title_short | Oxygen-carbon nanotubes as a chemotherapy sensitizer for paclitaxel in breast cancer treatment. |
| title_sort | oxygen carbon nanotubes as a chemotherapy sensitizer for paclitaxel in breast cancer treatment |
| url | http://europepmc.org/articles/PMC4121325?pdf=render |
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