Cytochrome P450 2E1 participation in the pathogenesis of experimental metabolic syndrome in guinea pigs
In this work the experimental metabolic syndrome on the basis of protamine sulfate modeling in guinea pigs was reproduced and pathological processes in the liver of experimental animals were studied. We determined the level of free radicals and markers of liver damage in the blood of experimental an...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2016-04-01
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| Series: | The Ukrainian Biochemical Journal |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2016/04/Rushchak_2_16.pdf |
| _version_ | 1797427762928025600 |
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| author | V. V. Rushchak M. O. Chashchyn |
| author_facet | V. V. Rushchak M. O. Chashchyn |
| author_sort | V. V. Rushchak |
| collection | DOAJ |
| description | In this work the experimental metabolic syndrome on the basis of protamine sulfate modeling in guinea pigs was reproduced and pathological processes in the liver of experimental animals were studied. We determined the level of free radicals and markers of liver damage in the blood of experimental animals. We investigated the liver glycogen content and K+,Na+-ATPase activity in the liver of experimental animals as well as measured the cytochrome P450 2E1 (CYP2E1) expression – one of the main factors of oxidative stress. Evidence of development of hepatotoxic processes, increasing of the CYP2E1 level as well as of the free radical level in the animals with metabolic syndrome were found. Using of CYP2E1 inhibitors had shown that the free radical level in the blood of experimental animals depended on the level of the enzyme expression and activity. The obtained results suggest that the changes in the CYP2E1 expression play an important role in the development of hepatotoxic processes upon experimental metabolic syndrome. It was assumed that pharmacological correction of the enzyme expression may be an important mechanism for the influence on the metabolic syndrome clinical course. |
| first_indexed | 2024-03-09T08:49:45Z |
| format | Article |
| id | doaj.art-293edf0eca044e6bb611beaef73eeca5 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T08:49:45Z |
| publishDate | 2016-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-293edf0eca044e6bb611beaef73eeca52023-12-02T14:35:55ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032016-04-018829810610.15407/ubj88.02.098Cytochrome P450 2E1 participation in the pathogenesis of experimental metabolic syndrome in guinea pigsV. V. Rushchak0M. O. Chashchyn1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, KyivInstitute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, KyivIn this work the experimental metabolic syndrome on the basis of protamine sulfate modeling in guinea pigs was reproduced and pathological processes in the liver of experimental animals were studied. We determined the level of free radicals and markers of liver damage in the blood of experimental animals. We investigated the liver glycogen content and K+,Na+-ATPase activity in the liver of experimental animals as well as measured the cytochrome P450 2E1 (CYP2E1) expression – one of the main factors of oxidative stress. Evidence of development of hepatotoxic processes, increasing of the CYP2E1 level as well as of the free radical level in the animals with metabolic syndrome were found. Using of CYP2E1 inhibitors had shown that the free radical level in the blood of experimental animals depended on the level of the enzyme expression and activity. The obtained results suggest that the changes in the CYP2E1 expression play an important role in the development of hepatotoxic processes upon experimental metabolic syndrome. It was assumed that pharmacological correction of the enzyme expression may be an important mechanism for the influence on the metabolic syndrome clinical course.http://ukrbiochemjournal.org/wp-content/uploads/2016/04/Rushchak_2_16.pdf |
| spellingShingle | V. V. Rushchak M. O. Chashchyn Cytochrome P450 2E1 participation in the pathogenesis of experimental metabolic syndrome in guinea pigs The Ukrainian Biochemical Journal |
| title | Cytochrome P450 2E1 participation in the pathogenesis of experimental metabolic syndrome in guinea pigs |
| title_full | Cytochrome P450 2E1 participation in the pathogenesis of experimental metabolic syndrome in guinea pigs |
| title_fullStr | Cytochrome P450 2E1 participation in the pathogenesis of experimental metabolic syndrome in guinea pigs |
| title_full_unstemmed | Cytochrome P450 2E1 participation in the pathogenesis of experimental metabolic syndrome in guinea pigs |
| title_short | Cytochrome P450 2E1 participation in the pathogenesis of experimental metabolic syndrome in guinea pigs |
| title_sort | cytochrome p450 2e1 participation in the pathogenesis of experimental metabolic syndrome in guinea pigs |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2016/04/Rushchak_2_16.pdf |
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