Antimicrobial Efficacy of Tubulicid Red as a Cavity Disinfectant in Comparison with Chlor-X Gel against S. mutans in Primary Teeth: Randomised Split-mouth Clinical Study
Introduction: Microorganisms act as precursors to dental caries. Following cavity preparation, traces of resident bacteria in the smear layer may contribute to the recurrence of caries. Thus, eliminating the underlying causes improves the likelihood of treatment success. Disinfectants are adjuva...
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| Format: | Article |
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JCDR Research and Publications Private Limited
2024-01-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://www.jcdr.net/articles/PDF/18885/64512_CE[Ra1]_F[SK]_QC&REF(AN_IS)_PF1(VD_KM_OM)_PFA(OM)_PN(KM).pdf |
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| author | swapnika vegi Veena Arali Harika Rapala Sritha Kolluri Sasidhar Vajrala |
| author_facet | swapnika vegi Veena Arali Harika Rapala Sritha Kolluri Sasidhar Vajrala |
| author_sort | swapnika vegi |
| collection | DOAJ |
| description | Introduction: Microorganisms act as precursors to dental
caries. Following cavity preparation, traces of resident bacteria
in the smear layer may contribute to the recurrence of caries.
Thus, eliminating the underlying causes improves the likelihood
of treatment success. Disinfectants are adjuvant tools that
should focus on removing microorganisms when combined with
procedures that yield positive outcomes.
Aim: To compare and evaluate the antimicrobial efficacy of
Tubulicid Red (TR) Label as a cavity disinfectant with Chlor X
gel (2% chlorhexidine) in primary teeth.
Materials and Methods: The present randomised double-blind
clinical trial was conducted at the Department of Paediatric and
Preventive Dentistry, GSL Dental College and Hospital, YSRUHS,
Rajahmundry, Andhra Pradesh, India from September 2021 to
April 2022. The study assessed the antimicrobial efficacy of TR
label against S. mutans in 60 primary carious teeth involving
1/3rd of the dentin. The teeth were divided into two groups,
each consisting of 30 teeth. Group-I received TR Label, while
Group-II received Chlor X. After excavating the infected dentine,
the affected dentine was left in the cavity, and a dentinal sample
was collected from the base of the cavity. The cavity was then
disinfected with either TR or Chlor X gel, followed by rinsing
with saline. A second sample was collected from the base of
the same cavity. The collected samples were transferred to
Eppendorf tubes and subjected to microbiological analysis to
calculate the number of Colony-forming Units (CFU) of S. mutans
on Mutans Sanguis (MS) agar medium. The obtained data were
subjected to statistical analysis using the Wilcoxon’s’s signedrank test and Mann-Whitney test.
Results: In the intragroup comparison (Wilcoxon’s signedrank test), both groups showed significant reductions in S.
mutans count (p<0.001). The intergroup comparison showed no
statistically significant difference (p>0.05) between the Tubulicid
and Chlor X groups at baseline and post-treatment.
Conclusion: The TR could be used as an alternative to Chlor X
gel as a cavity disinfectant in primary teeth before restoration,
potentially reducing the occurrence of secondary caries. |
| first_indexed | 2024-03-08T17:15:34Z |
| format | Article |
| id | doaj.art-2941b855f7d74b1bab6b8a120b221110 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-03-08T17:15:34Z |
| publishDate | 2024-01-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-2941b855f7d74b1bab6b8a120b2211102024-01-03T11:58:40ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2024-01-011801151810.7860/JCDR/2024/64512.18885Antimicrobial Efficacy of Tubulicid Red as a Cavity Disinfectant in Comparison with Chlor-X Gel against S. mutans in Primary Teeth: Randomised Split-mouth Clinical Studyswapnika vegi0Veena Arali1Harika Rapala2Sritha Kolluri3Sasidhar Vajrala4Postgraduate Student, Department of Pediatric and Preventive Dentistry, GSL Dental College and Hospital, YSRUHS, Rajahmundry, Andhra Pradesh, India.Professor and Head, Department of Pediatric and Preventive Dentistry, GSL Dental College and Hospital, YSRUHS, Rajahmundry, Andhra Pradesh, India.Reader, Department of Pediatric and Preventive Dentistry, GSL Dental College and Hospital, YSRUHS, Rajahmundry, Andhra Pradesh, India.Postgraduate Student, Department of Pediatric and Preventive Dentistry, GSL Dental College and Hospital, YSRUHS, Rajahmundry, Andhra Pradesh, India.Postgraduate Student, Department of Pediatric and Preventive Dentistry, GSL Dental College and Hospital, YSRUHS, Rajahmundry, Andhra Pradesh, India.Introduction: Microorganisms act as precursors to dental caries. Following cavity preparation, traces of resident bacteria in the smear layer may contribute to the recurrence of caries. Thus, eliminating the underlying causes improves the likelihood of treatment success. Disinfectants are adjuvant tools that should focus on removing microorganisms when combined with procedures that yield positive outcomes. Aim: To compare and evaluate the antimicrobial efficacy of Tubulicid Red (TR) Label as a cavity disinfectant with Chlor X gel (2% chlorhexidine) in primary teeth. Materials and Methods: The present randomised double-blind clinical trial was conducted at the Department of Paediatric and Preventive Dentistry, GSL Dental College and Hospital, YSRUHS, Rajahmundry, Andhra Pradesh, India from September 2021 to April 2022. The study assessed the antimicrobial efficacy of TR label against S. mutans in 60 primary carious teeth involving 1/3rd of the dentin. The teeth were divided into two groups, each consisting of 30 teeth. Group-I received TR Label, while Group-II received Chlor X. After excavating the infected dentine, the affected dentine was left in the cavity, and a dentinal sample was collected from the base of the cavity. The cavity was then disinfected with either TR or Chlor X gel, followed by rinsing with saline. A second sample was collected from the base of the same cavity. The collected samples were transferred to Eppendorf tubes and subjected to microbiological analysis to calculate the number of Colony-forming Units (CFU) of S. mutans on Mutans Sanguis (MS) agar medium. The obtained data were subjected to statistical analysis using the Wilcoxon’s’s signedrank test and Mann-Whitney test. Results: In the intragroup comparison (Wilcoxon’s signedrank test), both groups showed significant reductions in S. mutans count (p<0.001). The intergroup comparison showed no statistically significant difference (p>0.05) between the Tubulicid and Chlor X groups at baseline and post-treatment. Conclusion: The TR could be used as an alternative to Chlor X gel as a cavity disinfectant in primary teeth before restoration, potentially reducing the occurrence of secondary caries.https://www.jcdr.net/articles/PDF/18885/64512_CE[Ra1]_F[SK]_QC&REF(AN_IS)_PF1(VD_KM_OM)_PFA(OM)_PN(KM).pdfmicroorganismsmutans sanguis agar mediumsecondary cariesstreptococcus mutans |
| spellingShingle | swapnika vegi Veena Arali Harika Rapala Sritha Kolluri Sasidhar Vajrala Antimicrobial Efficacy of Tubulicid Red as a Cavity Disinfectant in Comparison with Chlor-X Gel against S. mutans in Primary Teeth: Randomised Split-mouth Clinical Study Journal of Clinical and Diagnostic Research microorganisms mutans sanguis agar medium secondary caries streptococcus mutans |
| title | Antimicrobial Efficacy of Tubulicid Red as a Cavity Disinfectant in Comparison with Chlor-X Gel against S. mutans in Primary Teeth: Randomised Split-mouth Clinical Study |
| title_full | Antimicrobial Efficacy of Tubulicid Red as a Cavity Disinfectant in Comparison with Chlor-X Gel against S. mutans in Primary Teeth: Randomised Split-mouth Clinical Study |
| title_fullStr | Antimicrobial Efficacy of Tubulicid Red as a Cavity Disinfectant in Comparison with Chlor-X Gel against S. mutans in Primary Teeth: Randomised Split-mouth Clinical Study |
| title_full_unstemmed | Antimicrobial Efficacy of Tubulicid Red as a Cavity Disinfectant in Comparison with Chlor-X Gel against S. mutans in Primary Teeth: Randomised Split-mouth Clinical Study |
| title_short | Antimicrobial Efficacy of Tubulicid Red as a Cavity Disinfectant in Comparison with Chlor-X Gel against S. mutans in Primary Teeth: Randomised Split-mouth Clinical Study |
| title_sort | antimicrobial efficacy of tubulicid red as a cavity disinfectant in comparison with chlor x gel against s mutans in primary teeth randomised split mouth clinical study |
| topic | microorganisms mutans sanguis agar medium secondary caries streptococcus mutans |
| url | https://www.jcdr.net/articles/PDF/18885/64512_CE[Ra1]_F[SK]_QC&REF(AN_IS)_PF1(VD_KM_OM)_PFA(OM)_PN(KM).pdf |
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