Reservoir of Antibiotic Residues and Resistant Coagulase Negative Staphylococci in a Healthy Population in the Greater Accra Region, Ghana
Antimicrobial resistance threatens infectious disease management outcomes, especially in developing countries. In this study, the occurrence of resistant coagulase-negative staphylococci (rCoNS) and antibiotic residues in urine samples of 401 healthy individuals from Korle-Gonno (KG) and Dodowa (DDW...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/1/119 |
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| author | Samuel Oppong Bekoe Sophie Hane-Weijman Sofie Louise Trads Emmanuel Orman Japheth Opintan Martin Hansen Niels Frimodt-Møller Bjarne Styrishave |
| author_facet | Samuel Oppong Bekoe Sophie Hane-Weijman Sofie Louise Trads Emmanuel Orman Japheth Opintan Martin Hansen Niels Frimodt-Møller Bjarne Styrishave |
| author_sort | Samuel Oppong Bekoe |
| collection | DOAJ |
| description | Antimicrobial resistance threatens infectious disease management outcomes, especially in developing countries. In this study, the occurrence of resistant coagulase-negative staphylococci (rCoNS) and antibiotic residues in urine samples of 401 healthy individuals from Korle-Gonno (KG) and Dodowa (DDW) in Ghana was investigated. MALDI-ToF/MS with gram-staining techniques detected and identified the CoNS. SPE-LC-MS/MS detected and quantified nine commonly used antibiotics in the samples. The results showed 63 CoNS isolates detected in 47 (12%) samples, with <i>S. haemolyticus</i> (78%) and <i>S. epidermidis</i> (8%) being predominant. Most of the isolates (95%) were resistant to at least one antibiotic, with the highest resistance observed against sulphamethoxazole (87%). Resistance profiles in samples from DDW and KG were largely comparable, but with some differences. For instance, DDW isolates were more resistant to gentamicin (<i>p</i> = 0.0244), trimethoprim (<i>p</i> = 0.0045), and cefoxitin (<i>p</i> = 0.0078), whereas KG isolates were more resistant to erythromycin (<i>p</i> = 0.0356). Although the volunteers had not knowingly consumed antibiotics two weeks before sampling, antibiotic residues, ranging between 1.44–17000 ng mL<sup>−1</sup> were identified in 22% of urine samples. Samples with antibiotic residues were likely to also contain rCoNS (89%). The most frequent antibiotics detected were tetracycline (63%) and ciprofloxacin (54%). Healthy individuals could thus be reservoirs of antibiotic residues and rCoNS at the community level. |
| first_indexed | 2024-03-10T03:01:31Z |
| format | Article |
| id | doaj.art-294b08ca5b5d424caf3941b7d8b8cdd4 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T03:01:31Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-294b08ca5b5d424caf3941b7d8b8cdd42023-11-23T12:45:44ZengMDPI AGAntibiotics2079-63822022-01-0111111910.3390/antibiotics11010119Reservoir of Antibiotic Residues and Resistant Coagulase Negative Staphylococci in a Healthy Population in the Greater Accra Region, GhanaSamuel Oppong Bekoe0Sophie Hane-Weijman1Sofie Louise Trads2Emmanuel Orman3Japheth Opintan4Martin Hansen5Niels Frimodt-Møller6Bjarne Styrishave7Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaToxicology and Drug Metabolism Group, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, DenmarkToxicology and Drug Metabolism Group, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, DenmarkDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Health and Allied Sciences, Ho, GhanaDepartment of Medical Microbiology, University of Ghana Medical School, Accra P.O. Box KB 4236, GhanaToxicology and Drug Metabolism Group, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, DenmarkDepartment of Clinical Microbiology, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, DenmarkToxicology and Drug Metabolism Group, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, DenmarkAntimicrobial resistance threatens infectious disease management outcomes, especially in developing countries. In this study, the occurrence of resistant coagulase-negative staphylococci (rCoNS) and antibiotic residues in urine samples of 401 healthy individuals from Korle-Gonno (KG) and Dodowa (DDW) in Ghana was investigated. MALDI-ToF/MS with gram-staining techniques detected and identified the CoNS. SPE-LC-MS/MS detected and quantified nine commonly used antibiotics in the samples. The results showed 63 CoNS isolates detected in 47 (12%) samples, with <i>S. haemolyticus</i> (78%) and <i>S. epidermidis</i> (8%) being predominant. Most of the isolates (95%) were resistant to at least one antibiotic, with the highest resistance observed against sulphamethoxazole (87%). Resistance profiles in samples from DDW and KG were largely comparable, but with some differences. For instance, DDW isolates were more resistant to gentamicin (<i>p</i> = 0.0244), trimethoprim (<i>p</i> = 0.0045), and cefoxitin (<i>p</i> = 0.0078), whereas KG isolates were more resistant to erythromycin (<i>p</i> = 0.0356). Although the volunteers had not knowingly consumed antibiotics two weeks before sampling, antibiotic residues, ranging between 1.44–17000 ng mL<sup>−1</sup> were identified in 22% of urine samples. Samples with antibiotic residues were likely to also contain rCoNS (89%). The most frequent antibiotics detected were tetracycline (63%) and ciprofloxacin (54%). Healthy individuals could thus be reservoirs of antibiotic residues and rCoNS at the community level.https://www.mdpi.com/2079-6382/11/1/119antimicrobial resistanceresistant CoNShealthy individualsLC-MS/MSantimicrobial susceptibility test |
| spellingShingle | Samuel Oppong Bekoe Sophie Hane-Weijman Sofie Louise Trads Emmanuel Orman Japheth Opintan Martin Hansen Niels Frimodt-Møller Bjarne Styrishave Reservoir of Antibiotic Residues and Resistant Coagulase Negative Staphylococci in a Healthy Population in the Greater Accra Region, Ghana Antibiotics antimicrobial resistance resistant CoNS healthy individuals LC-MS/MS antimicrobial susceptibility test |
| title | Reservoir of Antibiotic Residues and Resistant Coagulase Negative Staphylococci in a Healthy Population in the Greater Accra Region, Ghana |
| title_full | Reservoir of Antibiotic Residues and Resistant Coagulase Negative Staphylococci in a Healthy Population in the Greater Accra Region, Ghana |
| title_fullStr | Reservoir of Antibiotic Residues and Resistant Coagulase Negative Staphylococci in a Healthy Population in the Greater Accra Region, Ghana |
| title_full_unstemmed | Reservoir of Antibiotic Residues and Resistant Coagulase Negative Staphylococci in a Healthy Population in the Greater Accra Region, Ghana |
| title_short | Reservoir of Antibiotic Residues and Resistant Coagulase Negative Staphylococci in a Healthy Population in the Greater Accra Region, Ghana |
| title_sort | reservoir of antibiotic residues and resistant coagulase negative staphylococci in a healthy population in the greater accra region ghana |
| topic | antimicrobial resistance resistant CoNS healthy individuals LC-MS/MS antimicrobial susceptibility test |
| url | https://www.mdpi.com/2079-6382/11/1/119 |
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